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Mouse Recombinant Monoclonal HCN1 antibody. Suitable for Flow Cyt, WB, IHC-P, IHC-Fr and reacts with Human, Rat, Mouse samples. Cited in 18 publications. Immunogen corresponding to Recombinant Fragment Protein within Rat Hcn1 aa 750 to C-terminus.


Images

Flow Cytometry - Anti-HCN1 antibody [S70] (AB84816), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-HCN1 antibody [S70] (AB84816), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody [S70] (AB84816), expandable thumbnail
  • Western blot - Anti-HCN1 antibody [S70] (AB84816), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Fragment Protein within Rat Hcn1 aa 750 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9JKB0

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWBIHC-PIHC-Fr
Human
Tested
Expected
Tested
Tested
Mouse
Expected
Expected
Expected
Tested
Rat
Expected
Tested
Expected
Expected

Tested
Tested

Species
Human
Dilution info
1.00000-2.00000 µg for 106 Cells
Notes

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Rat
Dilution info
1.00000-10.00000 µg/mL
Notes

-

Expected
Expected

Species
Human, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse, Human
Dilution info
-
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Hyperpolarization-activated ion channel that are permeable to sodium and potassium ions (PubMed:15351778, PubMed:28086084). Displays lower selectivity for K(+) over Na(+) ions (PubMed:28086084). Contributes to the native pacemaker currents in heart (If) and in the generation of the I(h) current which controls neuron excitability (PubMed:29936235, PubMed:30351409). Participates in cerebellar mechanisms of motor learning (By similarity). May mediate responses to sour stimuli (By similarity).

Alternative names

BCNG1, HCN1, Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1, Brain cyclic nucleotide-gated channel 1, BCNG-1

Recommended products

Mouse Recombinant Monoclonal HCN1 antibody. Suitable for Flow Cyt, WB, IHC-P, IHC-Fr and reacts with Human, Rat, Mouse samples. Cited in 18 publications. Immunogen corresponding to Recombinant Fragment Protein within Rat Hcn1 aa 750 to C-terminus.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Fragment Protein within Rat Hcn1 aa 750 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9JKB0
Clone number
S70
Purification technique
Affinity purification Protein G
Specificity

No cross reactivity against HCN2

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

The clone number has been updated from S70-28 to N70/28, both clone numbers name the same antibody clone.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The HCN1 protein also known as hyperpolarization-activated cyclic nucleotide-gated channel 1 or HCN1 receptor is an important component in cellular electrical activity. It has a molecular mass of approximately 91 kDa. HCN1 channels are highly expressed in the heart and nervous system particularly in regions like the cortex and hippocampus. Their principal role is serving as pacemaker channels that regulate rhythmic activity in both neurons and cardiac cells.

Biological function summary

HCN1 channels contribute to the generation and modulation of electrical impulses. They belong to a family of ion channels activated by membrane hyperpolarization and modulated by cyclic nucleotides. These channels are part of a larger molecular complex that includes other HCN family members such as HCN2 and HCN4 which together generate coordinated ionic currents essential for maintaining the rhythmic activity.

Pathways

HCN1 channels significantly affect the pacing of cardiac and neuronal circuits. Within the cardiac tissue they partake in regulating heart rate through the If current or pacemaker current together with related proteins like HCN4. In the central nervous system their role extends to neuronal excitability and synaptic transmission interacting with signaling pathways that involve other ion channels and second messengers contributing to complex neuronal network activities.

Associated diseases and disorders

Alterations in HCN1 function or expression levels can lead to various conditions. In the heart dysregulation of HCN1 is associated with arrhythmias potentially involving interactions with proteins like the HCN4 channel. In the nervous system abnormal HCN1 activity has been linked to epilepsy with potential involvement of other ion channel dysfunctions. Understanding these associations provides insight into treatment strategies targeting these specific ion channel pathways.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Flow Cytometry - Anti-HCN1 antibody [S70] (ab84816), expandable thumbnail

    Flow Cytometry - Anti-HCN1 antibody [S70] (ab84816)

    Overlay histogram showing SH-SY5Y cells stained with ab84816 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab84816, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween for 20 min used under the same conditions.

  • Immunohistochemistry (Frozen sections) - Anti-HCN1 antibody [S70] (ab84816), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-HCN1 antibody [S70] (ab84816)

    Immunohistochemistry analysis of 10% formalin-fixed mouse frozen brain section labelling HCN1 with ab84816 at 1/1000 dilution for 1 hour at room temperature, followed by biotinylated Goat Anti-Mouse antibody (brown) for 30 minutes at room temperature. Counterstain: Hematoxylin (purple/blue) nuclear stain at 250-500 μl for 5 minutes at room temperature. HRP/DAB detection.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody [S70] (ab84816), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN1 antibody [S70] (ab84816)

    Immunohistochemical analysis of paraffin-embedded (Bouin's Fixative - picric acid, acetic acid and formaldehyde) human hippocampus tissue labelling HCN1 with ab84816 at 1/1000 dilution, for 1 hour at RT followed by secondary antibody Goat Anti-Mouse (FITC) at 1/50 dilution, for 1 hour at RT. Membrane and cytosolic staining on human hippocampus tissue is observed.

  • Western blot - Anti-HCN1 antibody [S70] (ab84816), expandable thumbnail

    Western blot - Anti-HCN1 antibody [S70] (ab84816)

    All lanes: Western blot - Anti-HCN1 antibody [S70] (ab84816) at 1/1000 dilution

    All lanes: Rat brain membrane lysates at 15 µg

    Secondary

    All lanes: HRP-Sheep Anti-Mouse IgG antibody

    Predicted band size: 99 kDa

    Observed band size: 99 kDa

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Product protocols

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