Rat Recombinant Monoclonal HCN2 antibody. Suitable for IHC-P and reacts with Mouse, Rat, Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN2 antibody [4A6] (AB252836), expandable thumbnail](https://content.abcam.com/products/images/hcn2-antibody-4a6-ab252836--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img22714.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN2 antibody [4A6] (AB252836)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN2 antibody [4A6] (AB252836), expandable thumbnail](https://content.abcam.com/products/images/hcn2-antibody-4a6-ab252836--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img110113.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN2 antibody [4A6] (AB252836)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN2 antibody [4A6] (AB252836), expandable thumbnail](https://content.abcam.com/products/images/hcn2-antibody-4a6-ab252836--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img14570.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN2 antibody [4A6] (AB252836)")
Key facts
- Isotype
IgG1
- Host species
Rat
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Not recommended | Tested |
| Mouse | Not recommended | Tested |
| Rat | Not recommended | Tested |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1.235 µg/mL | Notes Use at 1:200 dilution for human, 1:500 dilution for mouse and rat. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1.235 µg/mL | Notes Use at 1:200 dilution for human, 1:500 dilution for mouse and rat. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1.235 µg/mL | Notes Use at 1:200 dilution for human, 1:500 dilution for mouse and rat. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
1 products for Alternative Version
Target data
Function
Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Can also transport ammonium in the distal nephron. Produces a large instantaneous current. Modulated by intracellular chloride ions and pH; acidic pH shifts the activation to more negative voltages (By similarity).
Alternative names
Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2, Brain cyclic nucleotide-gated channel 2, BCNG-2, HCN2, BCNG2
Recommended products
Rat Recombinant Monoclonal HCN2 antibody. Suitable for IHC-P and reacts with Mouse, Rat, Human samples.
Alternative names
Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2, Brain cyclic nucleotide-gated channel 2, BCNG-2, HCN2, BCNG2
Key facts
- Isotype
IgG1
- Host species
Rat
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
4A6
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Supplementary info
- Activity summary
HCN2 also known as hyperpolarization-activated cyclic nucleotide-gated channel 2 is a protein that forms part of the HCN channel family. These channels are responsible for generating the I_h current in cardiac and neuronal tissues. The HCN2 protein has a molecular mass of approximately 97 kDa. It is prominently expressed in the heart and brain particularly in the sinoatrial node of the heart and various neurons in the brain's central nervous system.
- Biological function summary
The HCN2 channel influences the rhythmic activity of cardiac pacemaker cells and certain types of neurons. It belongs to a larger complex of ion channels that contribute to the cardiac pacemaking process and synchronization of neuronal firing. The HCN channels facilitate the flow of potassium and sodium ions which is critical for modulating electrical excitability and rhythmic oscillations in these cells.
- Pathways
HCN2 plays a significant role in both cardiac and neurological pathways. In the cardiac context it is part of the heart rate regulation pathway cooperating with other proteins such as HCN4 for managing the heart's rhythmic cycle. Neurologically HCN2 influences the pathway responsible for synaptic transmission and neuronal excitability interfacing with proteins like TRIP8b which modulate the activity of HCN channels in the neurons.
- Associated diseases and disorders
HCN2 shows connections to conditions such as cardiac arrhythmias and pain disorders. Disruptions in HCN2 function may lead to irregular heartbeats or contribute to chronic pain disorders due to its role in modulating electrical excitability. In pain-related pathways it interacts with other ion channels like Nav1.7 which are critical in the pathway of pain signaling and transmission across neurons.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN2 antibody [4A6] (ab252836), expandable thumbnail](https://content.abcam.com/products/images/hcn2-antibody-4a6-ab252836--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img22714.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN2 antibody [4A6] (ab252836)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN2 antibody [4A6] (ab252836)
Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling HCN2 with ab252836 at 1.235μg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Positive staining on rat cerebrum tissue is observed. The section was incubated with ab252836 for 30mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN2 antibody [4A6] (ab252836), expandable thumbnail](https://content.abcam.com/products/images/hcn2-antibody-4a6-ab252836--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img110113.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN2 antibody [4A6] (ab252836)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN2 antibody [4A6] (ab252836)
Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling HCN2 with ab252836 at 1.235μg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Positive staining on mouse cerebrum tissue is observed. The section was incubated with ab252836 for 30mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN2 antibody [4A6] (ab252836), expandable thumbnail](https://content.abcam.com/products/images/hcn2-antibody-4a6-ab252836--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img14570.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN2 antibody [4A6] (ab252836)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN2 antibody [4A6] (ab252836)
Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling HCN2 with ab252836 at 1.235μg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Positive staining on human cerebrum tissue is observed. The section was incubated with ab252836 for 30mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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