AB19346

Anti-HCN2 antibody

Name: Anti-HCN2 antibody (ab19346) | Abcam
Brand: Abcam Limited
SKU: AB19346

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(8 Publications)

Rabbit Polyclonal HCN2 antibody. Suitable for WB, ICC/IF and reacts with Rat, Mouse samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human HCN2 aa 850 to C-terminus.

View Alternative Names

BCNG2, HCN2, Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2, Brain cyclic nucleotide-gated channel 2, BCNG-2

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-HCN2 antibody (AB19346)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HCN2 antibody (AB19346)

Immunofluorescent analysis of Neuro-2a (Mouse neuroblastoma cell line) cells on 70% confluent log phase labeling HCN2. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labelled with ab19346 at 2 μg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) secondary antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution for 45 minutes at room temperature (Panel a : green). Nuclei (Panel b : blue) were stained with DAPI. F-actin (Panel c : red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (1/300 dilution). Panel d is a merged image showing localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.

Unknown

Western blot - Anti-HCN2 antibody (AB19346)

ab19346 has been successfully used in Western blot procedures. By Western blot, this antibody detects bands at ~100 kDa and ~115 kDa representing the non-glycosylated and glycosylated form of HCN2 in rat brain extract.

All lanes:

Western blot - Anti-HCN2 antibody (ab19346)

Predicted band size: 97 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human HCN2 aa 850 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9UL51

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HCN2 also known as hyperpolarization-activated cyclic nucleotide-gated channel 2 is a protein that forms part of the HCN channel family. These channels are responsible for generating the I_h current in cardiac and neuronal tissues. The HCN2 protein has a molecular mass of approximately 97 kDa. It is prominently expressed in the heart and brain particularly in the sinoatrial node of the heart and various neurons in the brain's central nervous system.

Biological function summary

The HCN2 channel influences the rhythmic activity of cardiac pacemaker cells and certain types of neurons. It belongs to a larger complex of ion channels that contribute to the cardiac pacemaking process and synchronization of neuronal firing. The HCN channels facilitate the flow of potassium and sodium ions which is critical for modulating electrical excitability and rhythmic oscillations in these cells.

Pathways

HCN2 plays a significant role in both cardiac and neurological pathways. In the cardiac context it is part of the heart rate regulation pathway cooperating with other proteins such as HCN4 for managing the heart's rhythmic cycle. Neurologically HCN2 influences the pathway responsible for synaptic transmission and neuronal excitability interfacing with proteins like TRIP8b which modulate the activity of HCN channels in the neurons.

HCN2 shows connections to conditions such as cardiac arrhythmias and pain disorders. Disruptions in HCN2 function may lead to irregular heartbeats or contribute to chronic pain disorders due to its role in modulating electrical excitability. In pain-related pathways it interacts with other ion channels like Nav1.7 which are critical in the pathway of pain signaling and transmission across neurons.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Hyperpolarization-activated ion channel that is permeable to sodium and potassium ions. Displays lower selectivity for K(+) over Na(+) ions (PubMed : 10228147, PubMed : 22006928). Contributes to the native pacemaker currents in heart (If) and in neurons (Ih) (PubMed : 10228147, PubMed : 10524219). Can also transport ammonium in the distal nephron (By similarity). Involved in the initiation of neuropathic pain in sensory neurons (By similarity).

See full target information HCN2

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

iScience 27:109744 PubMed38711442

2024

AKAP8 promotes ovarian cancer progression and antagonizes PARP inhibitor sensitivity through regulating hnRNPUL1 transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Youchaou Mobet,Haocheng Wang,Qinglv Wei,Xiaoyi Liu,Dan Yang,Hongyan Zhao,Yu Yang,Rosalie Anne Ngono Ngane,Jacob Souopgui,Jing Xu,Tao Liu,Ping Yi

Frontiers in physiology 11:587040 PubMed33240105

2020

Regulation of HCN2 Current by PI3K/Akt Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongju Lu,Hong Zhan Wang,Chris R Gordon,Lisa M Ballou,Richard Z Lin,Ira S Cohen

Molecular medicine reports 20:3406-3414 PubMed31432175

2019

Overexpression of the medium‑conductance calcium‑activated potassium channel (SK4) and the HCN2 channel to generate a biological pacemaker.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyi Zhao,Mei Yang,Fengyuan Wang,Ankang Yang,Qingyan Zhao,Xi Wang,Yanhong Tang,Teng Wang,Congxin Huang

Brain research 1717:204-213 PubMed30940440

2019

HCN channel antagonist ZD7288 ameliorates neuropathic pain and associated depression.

Applications

Unspecified application

Species

Unspecified reactive species

Lujia Yang,Xiaoyan Liu,Kai Yao,Yan Sun,Fan Jiang,Haitao Yan,Peng Mao,Shuangyi Fan,Xiaoli Wei,Ying Liu,Weixiu Yuan,Shuzhuo Zhang

Molecular medicine reports 19:2687-2697 PubMed30720107

2019

Implantation of engineered conduction tissue in the rat heart.

Applications

Unspecified application

Species

Unspecified reactive species

Wenbo Zhang,Xiaotong Li,Shanquan Sun,Xi Zhang

Molecular endocrinology (Baltimore, Md.) 26:153-64 PubMed22135067

2011

The expression and role of hyperpolarization-activated and cyclic nucleotide-gated channels in endocrine anterior pituitary cells.

Applications

Species

Rat

Karla Kretschmannova,Marek Kucka,Arturo E Gonzalez-Iglesias,Stanko S Stojilkovic

Experimental neurology 219:249-57 PubMed19500574

2009

Excitotoxic-mediated transcriptional decreases in HCN2 channel function increase network excitability in CA1.

Applications

Species

Unspecified reactive species

Brendan E L Adams,Christopher A Reid,Damian Myers,Caroline Ng,Kim Powell,A Marie Phillips,Thomas Zheng,Terence J O'Brien,David A Williams

The Journal of comparative neurology 501:891-903 PubMed17311321

2007

Differential distribution of hyperpolarization-activated and cyclic nucleotide-gated channels in cone bipolar cells of the rat retina.

Applications

Unspecified application

Species

Unspecified reactive species

Bozena Fyk-Kolodziej,Roberta G Pourcho

View all publications

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Product promise

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Anti-HCN2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-HCN2 antibody (AB19346)

Western blot - Anti-HCN2 antibody (AB19346)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Product promise