AB84817

Anti-HCN2 antibody [S71]

Name: Anti-HCN2 antibody [S71] (ab84817) | Abcam
Brand: Abcam Limited
SKU: AB84817
Price: 510 USD
Availability: InStock
Rating: 5 (4 reviews)

(4 Reviews)

(12 Publications)

Mouse Monoclonal HCN2 antibody. Suitable for WB, IHC-Fr and reacts with Mouse, Rat samples. Cited in 12 publications. Immunogen corresponding to Recombinant Protein within Rat Hcn2 aa 750 to C-terminus.

View Alternative Names

BCNG2, HCN2, Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2, Brain cyclic nucleotide-gated channel 2, BCNG-2

4 Images

Immunohistochemistry (Frozen sections) - Anti-HCN2 antibody [S71] (AB84817)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-HCN2 antibody [S71] (AB84817)

Immunohistochemistry (Frozen sections) analysis of mouse brain tissue labelling HCN2 with ab84817 at 1/1000 dilution (1 hour at room temperature). Tissue fixed with 10% Formalin Solution for 12-24 hours at room temperature. Secondary Antibody : HRP/DAB Detection System : Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at room temperature. Counterstained with Mayer hematoxylin (purple/blue) nuclear stain at 250-500 μL for 5 minutes at room temperature.

Supplier Data

Western blot - Anti-HCN2 antibody [S71] (AB84817)

All lanes:

Western blot - Anti-HCN2 antibody [S71] (ab84817) at 1/1000 dilution

All lanes:

Rat brain membrane lysates at 15 µg

Secondary

All lanes:

HRP-Sheep Anti-Mouse IgG antibody

Predicted band size: 97 kDa

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Supplier Data

Western blot - Anti-HCN2 antibody [S71] (AB84817)

Chemiluminescent for HRP (Moss) for 5 min in RT.

All lanes:

Western blot - Anti-HCN2 antibody [S71] (ab84817) at 1/1000 dilution

All lanes:

Mouse brain tissue lysate at 15 µg

Secondary

All lanes:

Goat anti-mouse IgG, HRP at 1/4000 dilution

Predicted band size: 97 kDa

Observed band size: 95 kDa

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CiteAb

Western blot - Anti-HCN2 antibody [S71] (AB84817)

HCN2 western blot using anti-HCN2 antibody [S71] ab84817. Publication image and figure legend from Feng, Q. F., Hou, Y. H., et al., 2013, Evid Based Complement Alternat Med, PubMed 24194780.

ab84817 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab84817 please see the product overview.

Relative expression level of the HCN2 protein in bladder determined by Western blot. * versus normal control group p < 0.05. △ versus model group p < 0.05.

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

S71

Isotype

IgG1

Carrier free

Reacts with

Mouse, Rat

Applications

WB, IHC-Fr

applications

Immunogen

Recombinant Protein within Rat Hcn2 aa 750 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9JKA9

Specificity

Does not cross react with HCN1.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/1000", "IHCFr-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Product details

The clone number S71 has previously been called S71-37 and N71/37. These clone numbers all refer to the same antibody clone.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HCN2 also known as hyperpolarization-activated cyclic nucleotide-gated channel 2 is a protein that forms part of the HCN channel family. These channels are responsible for generating the I_h current in cardiac and neuronal tissues. The HCN2 protein has a molecular mass of approximately 97 kDa. It is prominently expressed in the heart and brain particularly in the sinoatrial node of the heart and various neurons in the brain's central nervous system.

Biological function summary

The HCN2 channel influences the rhythmic activity of cardiac pacemaker cells and certain types of neurons. It belongs to a larger complex of ion channels that contribute to the cardiac pacemaking process and synchronization of neuronal firing. The HCN channels facilitate the flow of potassium and sodium ions which is critical for modulating electrical excitability and rhythmic oscillations in these cells.

Pathways

HCN2 plays a significant role in both cardiac and neurological pathways. In the cardiac context it is part of the heart rate regulation pathway cooperating with other proteins such as HCN4 for managing the heart's rhythmic cycle. Neurologically HCN2 influences the pathway responsible for synaptic transmission and neuronal excitability interfacing with proteins like TRIP8b which modulate the activity of HCN channels in the neurons.

HCN2 shows connections to conditions such as cardiac arrhythmias and pain disorders. Disruptions in HCN2 function may lead to irregular heartbeats or contribute to chronic pain disorders due to its role in modulating electrical excitability. In pain-related pathways it interacts with other ion channels like Nav1.7 which are critical in the pathway of pain signaling and transmission across neurons.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Hyperpolarization-activated ion channel that is permeable to sodium and potassium ions. Displays lower selectivity for K(+) over Na(+) ions (PubMed : 10228147, PubMed : 22006928). Contributes to the native pacemaker currents in heart (If) and in neurons (Ih) (PubMed : 10228147, PubMed : 10524219). Can also transport ammonium in the distal nephron (By similarity). Involved in the initiation of neuropathic pain in sensory neurons (By similarity).

See full target information HCN2

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

The Journal of neuroscience : the official journal of the Society for Neuroscience 44: PubMed39313321

2024

Selective Vulnerability of GABAergic Inhibitory Interneurons to Bilirubin Neurotoxicity in the Neonatal Brain.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Na Gong,Han-Wei Liu,Ke Lai,Zhen Zhang,Lin-Fei Mao,Zhen-Qi Liu,Ming-Xian Li,Xin-Lu Yin,Min Liang,Hai-Bo Shi,Lu-Yang Wang,Shan-Kai Yin

Neurochemistry international 162:105437 PubMed36336089

2022

Activation of HCN channels caused by elevated cAMP levels in periaqueductal gray promotes bone cancer pain.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaolu Lei,Yan Yan,Junwei Zeng,Rong Wang,Song Li,Zhi Xiao,Xiaohong Liu

Neural regeneration research 18:594-602 PubMed36018183

2022

Activation of metabotropic glutamate receptor 1 regulates hippocampal CA1 region excitability in rats with status epilepticus by suppressing the HCN1 channel.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Dan Luo,Tao Xiang,Si-Jun Li,Mei-Gang Ma,Mei-Ling Chen,Yuan Wu

Frontiers in pharmacology 12:647021 PubMed33995059

2021

The HCN Channel Blocker ZD7288 Induces Emesis in the Least Shrew ().

Applications

Unspecified application

Species

Unspecified reactive species

W Zhong,N A Darmani

Neuroscience research : PubMed32454040

2020

Shank3 contributes to neuropathic pain by facilitating the SNI-dependent increase of HCN2 and the expression of PSD95.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaofei Zhang,Xiaohui Li,Huan Wang,Xianqiao Xie,Yang Li,Xueqin Xu,Shanchun Su,Haiwen Zhao,Junhong Li,Changbin Ke,Juying Liu

Molecular medicine reports 20:3406-3414 PubMed31432175

2019

Overexpression of the medium‑conductance calcium‑activated potassium channel (SK4) and the HCN2 channel to generate a biological pacemaker.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyi Zhao,Mei Yang,Fengyuan Wang,Ankang Yang,Qingyan Zhao,Xi Wang,Yanhong Tang,Teng Wang,Congxin Huang

Frontiers in physiology 9:753 PubMed29971015

2018

Hyperpolarization-Activated Cyclic Nucleotide-Gated Non-selective (HCN) Ion Channels Regulate Human and Murine Urinary Bladder Contractility.

Applications

Unspecified application

Species

Unspecified reactive species

Felix Mader,Steffen Müller,Ludwig Krause,Armin Springer,Karoline Kernig,Chris Protzel,Katrin Porath,Simone Rackow,Tristan Wittstock,Marcus Frank,Oliver W Hakenberg,Rüdiger Köhling,Timo Kirschstein

The journal of pain : official journal of the Amer 19:626-634 PubMed29366880

2018

Increased HCN Channel Activity in the Gasserian Ganglion Contributes to Trigeminal Neuropathic Pain.

Applications

Unspecified application

Species

Unspecified reactive species

Weihua Ding,Zerong You,Shiqian Shen,Jinsheng Yang,Grewo Lim,Jason T Doheny,Shengmei Zhu,Yi Zhang,Lucy Chen,Jianren Mao

Communicative & integrative biology 10:e1309488 PubMed28702127

2017

Coordinating heart morphogenesis: A novel role for hyperpolarization-activated cyclic nucleotide-gated (HCN) channels during cardiogenesis in .

Applications

Unspecified application

Species

Unspecified reactive species

Emily Pitcairn,Hannah Harris,Justine Epiney,Vaibhav P Pai,Joan M Lemire,Bin Ye,Nian-Qing Shi,Michael Levin,Kelly A McLaughlin

Oncology letters 13:1601-1608 PubMed28454297

2017

Association between hyperpolarization-activated channel in interstitial cells of Cajal and gastrointestinal dysmotility induced by malignant ascites.

Applications

IHC-Fr

Species

Mouse

Tieyun Guo,Jiade Li,Jing Li,Dan Kong,Chunli Bi,Zheng He,Dai Tang,Xiaoming Jin,Lianhong Jin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HCN2 antibody [S71]

Immunohistochemistry (Frozen sections) - Anti-HCN2 antibody [S71] (AB84817)

Western blot - Anti-HCN2 antibody [S71] (AB84817)

Western blot - Anti-HCN2 antibody [S71] (AB84817)

Western blot - Anti-HCN2 antibody [S71] (AB84817)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

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Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

Product promise