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Rabbit Recombinant Monoclonal HCN3 antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-HCN3 antibody [EPR13494(2)] - BSA and Azide free (AB251107), expandable thumbnail
  • Western blot - Anti-HCN3 antibody [EPR13494(2)] - BSA and Azide free (AB251107), expandable thumbnail
  • Immunoprecipitation - Anti-HCN3 antibody [EPR13494(2)] - BSA and Azide free (AB251107), expandable thumbnail
  • Western blot - Anti-HCN3 antibody [EPR13494(2)] - BSA and Azide free (AB251107), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Human
Tested
Tested
Mouse
Expected
Tested
Rat
Expected
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Hyperpolarization-activated ion channel that are permeable to sodium and potassium ions, with an about 3:1 preference for potassium ions (PubMed:16043489). Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). In particular, plays a pivotal role in maintaining excitability and promoting rhythmic burst firing within hypothalamic nuclei. Exerts a significant influence on the configuration of the cardiac action potential waveform. Does not appear to play a prominent role in the processing of acute, neuropathic, or inflammatory pain (By similarity).

Alternative names

KIAA1535, HCN3, Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3

Recommended products

Rabbit Recombinant Monoclonal HCN3 antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR13494(2)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab251107 is the carrier-free version of Anti-HCN3 antibody [EPR13494(2)] ab192025.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The Hyperpolarization-activated Cyclic Nucleotide-gated channel 3 (HCN3) also known as potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3 is a protein that forms ion channels. These channels facilitate the flow of sodium and potassium ions across the cell membrane which is essential for maintaining the electrical activity in cells. HCN3 has a molecular mass of approximately 97 kDa and is expressed in various tissues including the brain and heart. Its role in creating the 'funny current' makes it important for electrical rhythms in these tissues.

Biological function summary

HCN3 channels contribute to pacemaker activities in neurons and cardiac cells regulating heartbeat and neural firing. HCN3 does not operate in isolation; it can be part of larger channel complexes influencing the excitability of cells. These channels act by responding to membrane hyperpolarization opening to allow ion flow that modulates cell activity and therefore impacts processes like synaptic transmission and cardio-excitatory responses.

Pathways

HCN3 channels integrate into signal transduction pathways involving cyclic nucleotides specifically the cAMP pathway. They interact with other hyperpolarization-activated cyclic nucleotide-gated channels like HCN1 and HCN2 which help refine electrical signaling. HCN3's involvement in the regulation of neural excitability links it to pathways governing neurotransmitter release and heart rate modulation.

Associated diseases and disorders

HCN3 potentially relates to epilepsy and cardiac arrhythmias. Aberrant HCN3 channel function can disrupt electrical signaling contributing to pathogenesis in these conditions. Interactions with other ion channels such as HCN2 may amplify disturbances in neural firing and cardiac pacing. Understanding HCN3’s functional mechanics offers insight into its role in disease manifestation and could guide therapeutic targeting.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-HCN3 antibody [EPR13494(2)] - BSA and Azide free (ab251107), expandable thumbnail

    Western blot - Anti-HCN3 antibody [EPR13494(2)] - BSA and Azide free (ab251107)

    This data was developed using Anti-HCN3 antibody [EPR13494(2)] ab192025, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-HCN3 antibody [EPR13494(2)] (Anti-HCN3 antibody [EPR13494(2)] ab192025) at 0.511 µg/mL

    Lane 1: Mouse brain tissue lysate at 20 µg

    Lane 2: Rat Brain Tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 0.05 µg/mL

    Predicted band size: 86 kDa

    Observed band size: 100 kDa

  • Western blot - Anti-HCN3 antibody [EPR13494(2)] - BSA and Azide free (ab251107), expandable thumbnail

    Western blot - Anti-HCN3 antibody [EPR13494(2)] - BSA and Azide free (ab251107)

    This data was developed using Anti-HCN3 antibody [EPR13494(2)] ab192025, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-HCN3 antibody [EPR13494(2)] (Anti-HCN3 antibody [EPR13494(2)] ab192025) at 1/10000 dilution

    Lane 1: Human cerebellum lysate at 20 µg

    Lane 2: Human fetal brain tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 86 kDa

    Observed band size: 90 kDa

  • Immunoprecipitation - Anti-HCN3 antibody [EPR13494(2)] - BSA and Azide free (ab251107), expandable thumbnail

    Immunoprecipitation - Anti-HCN3 antibody [EPR13494(2)] - BSA and Azide free (ab251107)

    This data was developed using Anti-HCN3 antibody [EPR13494(2)] ab192025, the same antibody clone in a different buffer formulation.Western blot analysis of HCN3 in Human fetal brain lysate immunoprecipitated using Anti-HCN3 antibody [EPR13494(2)] ab192025 at 1/30 dilution.Secondary antibody: Goat anti-Rabbit IgG (H+L), Peroxidase conjugated at 1/1000 dilution. Blocking/dilution buffer: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-HCN3 antibody [EPR13494(2)] (Anti-HCN3 antibody [EPR13494(2)] ab192025)

    Predicted band size: 86 kDa

  • Western blot - Anti-HCN3 antibody [EPR13494(2)] - BSA and Azide free (ab251107), expandable thumbnail

    Western blot - Anti-HCN3 antibody [EPR13494(2)] - BSA and Azide free (ab251107)

    This data was developed using Anti-HCN3 antibody [EPR13494(2)] ab192025, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-HCN3 antibody [EPR13494(2)] (Anti-HCN3 antibody [EPR13494(2)] ab192025) at 1/1000 dilution

    Lane 1: Human forebrain lysate at 10 µg

    Lane 2: Human hypothalamus lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 86 kDa

    Observed band size: 90 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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