AB251107

Anti-HCN3 antibody [EPR13494(2)] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal HCN3 antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse, Rat samples.

View Alternative Names

KIAA1535, HCN3, Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3

4 Images

Supplier Data

Immunoprecipitation - Anti-HCN3 antibody [EPR13494(2)] - BSA and Azide free (AB251107)

This data was developed using ab192025, the same antibody clone in a different buffer formulation.

Western blot analysis of HCN3 in Human fetal brain lysate immunoprecipitated using ab192025 at 1/30 dilution.

Secondary antibody : Goat anti-Rabbit IgG (H+L), Peroxidase conjugated at 1/1000 dilution.

Blocking/dilution buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-HCN3 antibody [EPR13494(2)] (<a href='/en-us/products/primary-antibodies/hcn3-antibody-epr134942-ab192025'>ab192025</a>)

Predicted band size: 86 kDa

false

Supplier Data

Western blot - Anti-HCN3 antibody [EPR13494(2)] - BSA and Azide free (AB251107)

This data was developed using ab192025, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-HCN3 antibody [EPR13494(2)] (<a href='/en-us/products/primary-antibodies/hcn3-antibody-epr134942-ab192025'>ab192025</a>) at 1/10000 dilution

Lane 1:

Human cerebellum lysate at 20 µg

Lane 2:

Human fetal brain tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 86 kDa

Observed band size: 90 kDa

false

Supplier Data

Western blot - Anti-HCN3 antibody [EPR13494(2)] - BSA and Azide free (AB251107)

This data was developed using ab192025, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-HCN3 antibody [EPR13494(2)] (<a href='/en-us/products/primary-antibodies/hcn3-antibody-epr134942-ab192025'>ab192025</a>) at 1/1000 dilution

Lane 1:

Human forebrain lysate at 10 µg

Lane 2:

Human hypothalamus lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 86 kDa

Observed band size: 90 kDa

false

Lab

Western blot - Anti-HCN3 antibody [EPR13494(2)] - BSA and Azide free (AB251107)

This data was developed using ab192025, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-HCN3 antibody [EPR13494(2)] (<a href='/en-us/products/primary-antibodies/hcn3-antibody-epr134942-ab192025'>ab192025</a>) at 0.511 µg/mL

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Rat Brain Tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 0.05 µg/mL

Predicted band size: 86 kDa

Observed band size: 100 kDa

false

Unconjugated

Anti-HCN3 antibody [EPR13494(2)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13494(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab251107 is the carrier-free version of ab192025.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Hyperpolarization-activated Cyclic Nucleotide-gated channel 3 (HCN3) also known as potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3 is a protein that forms ion channels. These channels facilitate the flow of sodium and potassium ions across the cell membrane which is essential for maintaining the electrical activity in cells. HCN3 has a molecular mass of approximately 97 kDa and is expressed in various tissues including the brain and heart. Its role in creating the 'funny current' makes it important for electrical rhythms in these tissues.

Biological function summary

HCN3 channels contribute to pacemaker activities in neurons and cardiac cells regulating heartbeat and neural firing. HCN3 does not operate in isolation; it can be part of larger channel complexes influencing the excitability of cells. These channels act by responding to membrane hyperpolarization opening to allow ion flow that modulates cell activity and therefore impacts processes like synaptic transmission and cardio-excitatory responses.

Pathways

HCN3 channels integrate into signal transduction pathways involving cyclic nucleotides specifically the cAMP pathway. They interact with other hyperpolarization-activated cyclic nucleotide-gated channels like HCN1 and HCN2 which help refine electrical signaling. HCN3's involvement in the regulation of neural excitability links it to pathways governing neurotransmitter release and heart rate modulation.

HCN3 potentially relates to epilepsy and cardiac arrhythmias. Aberrant HCN3 channel function can disrupt electrical signaling contributing to pathogenesis in these conditions. Interactions with other ion channels such as HCN2 may amplify disturbances in neural firing and cardiac pacing. Understanding HCN3’s functional mechanics offers insight into its role in disease manifestation and could guide therapeutic targeting.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Hyperpolarization-activated ion channel that are permeable to sodium and potassium ions, with an about 3 : 1 preference for potassium ions (PubMed : 16043489). Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). In particular, plays a pivotal role in maintaining excitability and promoting rhythmic burst firing within hypothalamic nuclei. Exerts a significant influence on the configuration of the cardiac action potential waveform. Does not appear to play a prominent role in the processing of acute, neuropathic, or inflammatory pain (By similarity).

See full target information HCN3

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HCN3 antibody [EPR13494(2)] - BSA and Azide free

Immunoprecipitation - Anti-HCN3 antibody [EPR13494(2)] - BSA and Azide free (AB251107)

Western blot - Anti-HCN3 antibody [EPR13494(2)] - BSA and Azide free (AB251107)

Western blot - Anti-HCN3 antibody [EPR13494(2)] - BSA and Azide free (AB251107)

Western blot - Anti-HCN3 antibody [EPR13494(2)] - BSA and Azide free (AB251107)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise