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Mouse Recombinant Monoclonal HCN4 antibody. Suitable for IHC-P and reacts with Mouse, Rat samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN4 antibody [N114/10] (AB302675), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN4 antibody [N114/10] (AB302675), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN4 antibody [N114/10] (AB302675), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN4 antibody [N114/10] (AB302675), expandable thumbnail

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PIHC-FrICC/IF
Human
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Not recommended
Tested
Not recommended
Not recommended
Rat
Not recommended
Tested
Not recommended
Not recommended

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Rat, Mouse, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Target data

Function

Hyperpolarization-activated ion channel that are permeable to Na(+) and K(+) ions with very slow activation and inactivation. Exhibits higher selectivity for K(+) over Na(+) ions (By similarity). Contributes to the native pacemaker currents in heart (If) that regulate the rhythm of heart beat (PubMed:14657344, PubMed:21220308). Contributes to the native pacemaker currents in neurons (Ih) (By similarity). May mediate responses to sour stimuli (PubMed:11675786).

Alternative names

Bcng3, Hcn4, Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4, Brain cyclic nucleotide-gated channel 3, BCNG-3

Recommended products

Mouse Recombinant Monoclonal HCN4 antibody. Suitable for IHC-P and reacts with Mouse, Rat samples.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
N114-10
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

HCN4 also known as hyperpolarization-activated cyclic nucleotide-gated channel 4 is a protein that functions mechanically as an ion channel. It aids in carrying the hyperpolarization-activated non-selective cation current also called I_f or funny current. HCN4 has a molecular mass of about 136 kDa. The channel is expressed prominently in heart tissues particularly in the sinoatrial node which plays an important role in pacemaker activity to regulate heart rate. HCN4 also appears in some regions of the central nervous system and contributes to neural activity.

Biological function summary

Hyperpolarization-activated ion currents mediated by HCN4 contribute to the pacing of cardiac cells. They allow controlled influx of Na^+ and K^+ ions therefore facilitating rhythmic cardiac activity. Although it does not form part of a larger complex HCN4 interacts with auxiliary subunits and regulator proteins that can modulate its activity. It can be influenced by internal cyclic AMP levels leading to changes in its kinetics and voltage dependency.

Pathways

The involvement of HCN4 mostly figures into cardiac conduction and neuronal signaling pathways. Within the cardiac conduction system it plays a pivotal role in maintaining coordinated heart rhythms. HCN4's function relates to proteins like HCN1 HCN2 and HCN3 which together form a family responsible for various rhythmic activities in the heart and brain. It also assists in stabilizing neuronal firing patterns influenced by neurotransmitter-mediated effects but has less direct interaction with these related proteins.

Associated diseases and disorders

Mutations or dysfunctions in HCN4 can lead to cardiac arrhythmias like bradycardia. Abnormalities in the function of this protein can also contribute to sick sinus syndrome a condition characterized by problems with the sinoatrial node that affects heart rhythm. In these scenarios HCN4's interaction with other channel proteins and regulatory molecules becomes important as interventions might target these associated pathways for treatment.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN4 antibody [N114/10] (ab302675), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN4 antibody [N114/10] (ab302675)

    Immunohistochemical analysis of paraffin-embedded Mouse thalamus tissue labeling HCN4 with ab302675 at 1/500 (2.084 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on mouse thalamus.The section was incubated with ab302675 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913) for 8 mins during the LeicaDS9800 kit staining procedure.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN4 antibody [N114/10] (ab302675), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN4 antibody [N114/10] (ab302675)

    Immunohistochemical analysis of paraffin-embedded Mouse brain tissue labeling HCN4 with ab302675 at 1/500 (2.084 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on mouse brain.The section was incubated with ab302675 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913) for 8 mins during the LeicaDS9800 kit staining procedure.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN4 antibody [N114/10] (ab302675), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN4 antibody [N114/10] (ab302675)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling HCN4 with ab302675 at 1/500 (2.084 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control: No staining on rat liver.The section was incubated with ab302675 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913) for 8 mins during the LeicaDS9800 kit staining procedure.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN4 antibody [N114/10] (ab302675), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HCN4 antibody [N114/10] (ab302675)

    Immunohistochemical analysis of paraffin-embedded Rat thalamus tissue labeling HCN4 with ab302675 at 1/500 (2.084 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on rat thalamus.The section was incubated with ab302675 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913) for 8 mins during the LeicaDS9800 kit staining procedure.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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