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AB223085

Anti-hCNT3 antibody

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(4 Publications)

Rabbit Polyclonal hCNT3 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SLC28A3 aa 1-150.

View Alternative Names

CNT3, SLC28A3, Solute carrier family 28 member 3, Concentrative Na(+)-nucleoside cotransporter 3, CNT 3, hCNT3

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hCNT3 antibody (AB223085)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hCNT3 antibody (AB223085)

Paraffin embedded human colon cancer tissue stained for hCNT3 with ab223085 (1/100 dilution) in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-hCNT3 antibody (AB223085)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-hCNT3 antibody (AB223085)

HepG2 (human liver hepatocellular carcinoma cell line) cells stained for hCNT3 (green) using ab223085 (1/100 dilution) in ICC/IF. Secondary : Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hCNT3 antibody (AB223085)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hCNT3 antibody (AB223085)

Paraffin embedded human testis tissue stained for hCNT3 with ab223085 (1/100 dilution) in immunohistochemical analysis.

Western blot - Anti-hCNT3 antibody (AB223085)
  • WB

Supplier Data

Western blot - Anti-hCNT3 antibody (AB223085)

All lanes:

Western blot - Anti-hCNT3 antibody (ab223085) at 1/1000 dilution

All lanes:

mouse lung tissue lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 77 kDa

Observed band size: 77 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human SLC28A3 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

Q9HAS3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Human concentrative nucleoside transporter 3 (hCNT3) also known as SLC28A3 is responsible for the active transport of nucleosides across the cellular membrane. It is a member of the concentrative nucleoside transporter family and functions as a symporter using sodium or proton gradients to transport substrates. The protein has a mass of approximately 76 kDa. Its expression occurs widely with higher levels seen in the small intestine liver kidney and pancreas where high nucleotide metabolism activity is required.
Biological function summary

Human concentrative nucleoside transporter 3 plays an important role in cellular uptake of nucleosides and nucleoside analog drugs. These substrates are necessary for nucleotide synthesis and DNA replication. hCNT3 is not known to be a part of a larger protein complex but collaborates closely with intracellular enzymes that process nucleotides. Its ability to transport therapeutic nucleoside analogs makes it significant in pharmacology particularly in the treatment of cancer and viral infections.

Pathways

Human concentrative nucleoside transporter 3 is an important component in nucleoside salvage pathways. This is important for nucleotide recycling within cells ensuring adequate supply of DNA and RNA precursors. hCNT3 also interacts functionally with other transporter proteins like hCNT1 and hCNT2 which handle different substrate specificities. This cooperation ensures a wide range of nucleosides are able to cross cell membranes demonstrating the integration of hCNT3 in broader nucleotide metabolic processes.

Human concentrative nucleoside transporter 3 has links to certain cancer types and genetic metabolic disorders. Its ability to uptake nucleoside analog drugs means it affects chemotherapeutic efficiency and resistance in cancers like leukemia. Also mutations in the gene encoding hCNT3 may lead to altered drug metabolism impacting treatment outcomes. The transporter's interaction with other transport proteins such as hENT1 can compound these effects since they may influence drug uptake and retention at cellular levels.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Sodium-dependent, pyrimidine- and purine-selective (PubMed : 11032837, PubMed : 15861042, PubMed : 16446384, PubMed : 17140564, PubMed : 21998139). Involved in the homeostasis of endogenous nucleosides (PubMed : 11032837, PubMed : 15861042). Exhibits the transport characteristics of the nucleoside transport system cib or N3 subtype (N3/cib) (with marked transport of both thymidine and inosine) (PubMed : 11032837). Employs a 2 : 1 sodium/nucleoside ratio (PubMed : 11032837). Transports uridine (PubMed : 21795683). Also able to transport gemcitabine, 3'-azido-3'-deoxythymidine (AZT), ribavirin and 3-deazauridine (PubMed : 11032837, PubMed : 17140564).
See full target information SLC28A3
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:975 PubMed40595410

2025

circGIGYF1 inhibits stemness and metastasis in colorectal cancer by promoting WWP2-HOXD13 interaction to regulate β-catenin signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Zhao,Jiacheng Li,Zunxian Wang,Hairui Zhou,Zhuoxin Cheng,Shuxia Ma

Biochemical and biophysical research communications 558:120-125 PubMed33910126

2021

Characterization of deoxyribonucleoside transport mediated by concentrative nucleoside transporters.

Applications

Unspecified application

Species

Unspecified reactive species

Taiki Yamamura,Katsuya Narumi,Tsukika Ohata,Hiroshi Satoh,Takao Mori,Ayako Furugen,Masaki Kobayashi,Ken Iseki

Journal of nuclear cardiology : official publicati : PubMed31741324

2019

Cell proliferation detected using [F]FLT PET/CT as an early marker of abdominal aortic aneurysm.

Applications

Unspecified application

Species

Unspecified reactive species

Richa Gandhi,Christopher Cawthorne,Lucinda J L Craggs,John D Wright,Juozas Domarkas,Ping He,Joanna Koch-Paszkowski,Michael Shires,Andrew F Scarsbrook,Stephen J Archibald,Charalampos Tsoumpas,Marc A Bailey

PloS one 14:e0223892 PubMed31634358

2019

Mutual role of ecto-5'-nucleotidase/CD73 and concentrative nucleoside transporter 3 in the intestinal uptake of dAMP.

Applications

Unspecified application

Species

Unspecified reactive species

Katsuya Narumi,Tsukika Ohata,Yuichi Horiuchi,Hiroshi Satoh,Ayako Furugen,Masaki Kobayashi,Ken Iseki
View all publications
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