AB316286

Anti-HCoV-229E Nucleocapsid protein antibody [abd210] - BSA and Azide free

Recombinant
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Human Recombinant Monoclonal NCAP antibody. Carrier free. Suitable for ICC/IF, I-ELISA and reacts with Transfected cell line - Human coronavirus 229E, Recombinant fragment - Human coronavirus 229E samples.

View Alternative Names

7, N, Nucleoprotein, Nucleocapsid protein, NC, Protein N

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-HCoV-229E Nucleocapsid protein antibody [abd210] - BSA and Azide free (AB316286)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HCoV-229E Nucleocapsid protein antibody [abd210] - BSA and Azide free (AB316286)

This data was developed using ab316285, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) transfected with a HCoV-229E Nucleocapsid protein expression vector containing a myc tag. cells labelling HCoV-229E Nucleocapsid protein with ab316285 at 1/200 (4.985 ug/ml) dilution, followed by Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody (Alexa Fluor® 488) antibody at 1/1000 (2ug/ml) dilution (Green).

Confocal image showing cytoplasmic staining in 293T cells (shown in green) transfected with HCoV-229E Nucleocapsid protein expression vector containing a myc-His-tag®. The counterstain was observed in red. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab223895 Anti-Myc tag Mouse monoclonal antibody (Alexa Fluor® 647) was used to counterstain at 1/100 (5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody (Alexa Fluor® 488) at 1/1000 (2ug/ml) dilution.

Indirect ELISA - Anti-HCoV-229E Nucleocapsid protein antibody [abd210] - BSA and Azide free (AB316286)

I-ELISA

Supplier Data

Indirect ELISA - Anti-HCoV-229E Nucleocapsid protein antibody [abd210] - BSA and Azide free (AB316286)

This data was developed using ab316285, the same antibody clone in a different buffer formulation.

Indirect ELISA analysis of ab316285 at 2000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Human IgG (H+L) at 1 : 1000 dilution.

Antigen : HCoV-229E Nucleocapsid protein,HCoV-NL63 Nucleocapsid protein,SARS-CoV Nucleocapsid protein,HCoV-OC43 Nucleocapsid protein,HCoV-HKU1 Nucleocapsid protein,MERS-CoV Nucleocapsid protein.

Antigen concentration : 5000 ng/ml

Unconjugated

Anti-HCoV-229E Nucleocapsid protein antibody [abd210]

Key facts

Host species

Human

Clonality

Monoclonal

Clone number

abd210

Isotype

IgG1

Carrier free

Yes

Reacts with

Human coronavirus 229E

Applications

I-ELISA, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody does not cross-react with recombinant Human coronavirus NL63 nucleocapsid, MERS-CoV nucleocapsid, SARS-CoV nucleocapsid, Human coronavirus OC43 nucleocapsid and Human coronavirus HKU1-Cov nucleocapsid.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human coronavirus 229E": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IELISA-species-checked": "predicted", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Recombinant fragment - Human coronavirus 229E": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p></p>" }, "Transfected cell line - Human coronavirus 229E": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" } } }

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Product details

ab316286 is the carrirer-free version of ab316285.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The HCoV-229E Nucleocapsid protein also known as the N protein plays a mechanical role within the human coronavirus 229E (HCoV-229E). This protein has a mass of around 45 kDa and is expressed in the cytoplasm of infected host cells. Its primary function lies in binding to the viral RNA genome forming a ribonucleoprotein complex that protects the RNA and contributes to the stability of the viral genome. The HCoV-229E nucleocapsid protein is important for the correct assembly and packaging of the viral RNA into new virions.

Biological function summary

The N protein directly impacts viral replication and transcription. It forms part of a larger ribonucleoprotein complex that organizes the viral genome into a functional and protected structure. This complex interacts with other viral proteins to facilitate the replication process. The N protein also boosts the efficiency of the viral RdRp (RNA-dependent RNA polymerase) in transcribing the RNA genome ensuring that viral replication proceeds efficiently and effectively within host cells.

Pathways

The N protein integrates into the coronavirus replication-transcription complex. It coordinates with the viral RdRp and other non-structural proteins of the virus to ensure a streamlined replication process. This protein interacts with cellular pathways that include stress response and immune pathways potentially modulating host immune responses to benefit viral survival and replication. These interactions with host cell pathways may influence the efficiency of viral replication and the pathogenicity of the virus.

HCoV-229E Nucleocapsid protein connects to common cold manifestations and in some cases exacerbates chronic respiratory disorders. The N protein’s interactions with other viral proteins like the Spike protein might enhance viral entry and infectivity which could contribute to varying severity of infection. Understanding the role of the N protein in these contexts is important for developing therapeutic strategies and improving disease management for infections caused by HCoV-229E.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication.

See full target information N

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HCoV-229E Nucleocapsid protein antibody [abd210] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-HCoV-229E Nucleocapsid protein antibody [abd210] - BSA and Azide free (AB316286)

Indirect ELISA - Anti-HCoV-229E Nucleocapsid protein antibody [abd210] - BSA and Azide free (AB316286)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Complementary Products

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise