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AB309964

Anti-HCoV-OC43 Nucleoprotein antibody [abd384]

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Human Recombinant Monoclonal NCAP antibody. Suitable for WB, ICC/IF, I-ELISA and reacts with Human coronavirus OC43, Transfected cell lysate - Human coronavirus OC43, Recombinant fragment - Human coronavirus OC43, Transfected cell line - Human coronavirus OC43 samples.

View Alternative Names

7a, N, Nucleoprotein, Nucleocapsid protein, NC, Protein N

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-HCoV-OC43 Nucleoprotein antibody [abd384] (AB309964)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HCoV-OC43 Nucleoprotein antibody [abd384] (AB309964)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293T (human epithelial cell line from embryonic kidney) transfected with a Nucleoprotein expression vector containing a myc-tag. cells labelling HCoV-OC43 Nucleoprotein with ab309964 at 1/100 (10.87 ug/ml) dilution, followed by Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody (Alexa Fluor® 488) antibody at 1/100 dilution (Green). Confocal image showing cytoplasmic staining in 293T cells transfected with HCoV-OC43 Nucleoprotein expression vector containing a myc tag. No staining on HEK-293T transfected with empty vector containing a myc-tag. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab223895 Anti-Myc tag Mouse monoclonal antibody (Alexa Fluor® 647) was used to counterstain tubulin at 1/100 dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody (Alexa Fluor® 488) at 1/100 dilution.

Indirect ELISA - Anti-HCoV-OC43 Nucleoprotein antibody [abd384] (AB309964)
  • I-ELISA

Supplier Data

Indirect ELISA - Anti-HCoV-OC43 Nucleoprotein antibody [abd384] (AB309964)

Indirect ELISA analysis of ab309964 at 2000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Human IgG (H+L) at 1000 dilution. Antigen : HCoV-OC43 nucleocapsid His-tag protein. Antigen concentration : 5000 ng/ml.

Western blot - Anti-HCoV-OC43 Nucleoprotein antibody [abd384] (AB309964)
  • WB

Supplier Data

Western blot - Anti-HCoV-OC43 Nucleoprotein antibody [abd384] (AB309964)

Blocking and diluting buffer and concentration : 5% NFDM/TBST In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) was used at 1 : 5000 dilution.

All lanes:

Western blot - Anti-HCoV-OC43 Nucleoprotein antibody [abd384] (ab309964) at 1/1000 dilution

All lanes:

His-tagged HCoV-OC43 Nucleoprotein recombinant protein at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Human IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-human-igg-h-l-hrp-ab6858'>ab6858</a>) at 1/5000 dilution

Observed band size: 65 kDa

false

Exposure time: 3min

Western blot - Anti-HCoV-OC43 Nucleoprotein antibody [abd384] (AB309964)
  • WB

Supplier Data

Western blot - Anti-HCoV-OC43 Nucleoprotein antibody [abd384] (AB309964)

Blocking and diluting buffer and concentration : 5% NFDM/TBST In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) was used at 1 : 200000 dilution. In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) was used at 1 : 5000 dilution.

All lanes:

Western blot - Anti-HCoV-OC43 Nucleoprotein antibody [abd384] (ab309964) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cells) transfected with an empty vector containing a His-tag, whole cell lysate at 4 µg

Lane 2:

293T (human embryonic kidney epithelial cells) transfected with HCoV-OC43 Nucleoprotein expression vector containing a His-tag, whole cell lysate at 4 µg

Secondary

All lanes:

Western blot - Goat Anti-Human IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-human-igg-h-l-hrp-ab6858'>ab6858</a>) at 1/5000 dilution

Observed band size: 65 kDa

false

Exposure time: 1s

Key facts

Host species

Human

Clonality

Monoclonal

Clone number

abd384

Isotype

IgG1

Carrier free

No

Reacts with

Human coronavirus OC43

Applications

ICC/IF, WB, I-ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody does not cross-react with recombinant HCoV-NL63 nucleocapsid, MERS-CoV nucleocapsid, SARS-CoV nucleocapsid, HKU1 nucleocapsid and HCoV-229E nucleocapsid.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human coronavirus OC43": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Recombinant fragment - Human coronavirus OC43": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "2000 ng/mL", "IELISA-species-notes": "<p></p>" }, "Transfected cell line - Human coronavirus OC43": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Transfected cell lysate - Human coronavirus OC43": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HCoV-OC43 Nucleoprotein also known as OC43 N protein is a major structural component of the HCoV-OC43 coronavirus. This nucleoprotein has a mass of approximately 50 kDa. It is expressed within the infected host cells mainly in the cytoplasm where it plays an integral role in viral replication and assembly. The HCoV-OC43 Nucleoprotein ensures encapsidation of the viral RNA genome forming the ribonucleocapsid which is critical for protecting the viral RNA and aiding in its functionality within the cell.
Biological function summary

The HCoV-OC43 Nucleoprotein participates in forming complexes with viral RNA and other viral proteins. These interactions facilitate the replication cycle of HCoV-OC43 by regulating RNA transcription and synthesis. Additionally it is known to induce host responses influencing cellular processes and immune evasion mechanisms. This interaction with the host's cellular machinery enables the virus to establish infection and maintain its replication efficiency.

Pathways

HCoV-OC43 Nucleoprotein participates in essential viral life cycle pathways specifically in the replication and transcription complex formation. This protein associates with other viral entities like the RNA-dependent RNA polymerase and matrix protein M. Together they ensure the synthesis of subgenomic mRNA and full-length genomic RNAn important for the successful production of new virions. Understanding these pathways helps clarify how coronaviruses like HCoV-OC43 propagate and sustain infection within host cells.

HCoV-OC43 Nucleoprotein associates with respiratory infections including common colds and occasionally severe lower respiratory tract diseases. It plays a role in modulating host immune responses that contribute to disease severity. It often connects with host protein interactions such as those involving the immune modulator protein interferon which can either bolster or subvert immune defenses. Studies of HCoV-OC43 and its Nucleoprotein provide insight into coronavirus pathogenicity and potential therapeutic targets for viral respiratory illnesses.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Attenuates the stress granules formation by reducing host G3BP1 access to host mRNAs under stress conditions (PubMed : 36534661).
See full target information N
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Product promise

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