Anti-HCoV-OC43 Nucleoprotein antibody [abd384] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HCoV-OC43 Nucleoprotein antibody [abd384] - BSA and Azide free (AB309965)

This data was developed using ab309964 the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293T (human epithelial cell line from embryonic kidney) transfected with a Nucleoprotein expression vector containing a myc-tag. cells labelling HCoV-OC43 Nucleoprotein with ab309964 at 1/100 (10.87 ug/ml) dilution, followed by Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody (Alexa Fluor® 488) antibody at 1/100 dilution (Green). Confocal image showing cytoplasmic staining in 293T cells transfected with HCoV-OC43 Nucleoprotein expression vector containing a myc tag. No staining on HEK-293T transfected with empty vector containing a myc-tag. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab223895 Anti-Myc tag Mouse monoclonal antibody (Alexa Fluor® 647) was used to counterstain tubulin at 1/100 dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody (Alexa Fluor® 488) at 1/100 dilution.

• I-ELISA

Supplier Data

Indirect ELISA - Anti-HCoV-OC43 Nucleoprotein antibody [abd384] - BSA and Azide free (AB309965)

This data was developed using ab309964, the same antibody clone in a different buffer formulation. Indirect ELISA analysis of ab309964 at 2000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Human IgG (H+L) at 1000 dilution. Antigen : HCoV-OC43 nucleocapsid His-tag protein. Antigen concentration : 5000 ng/ml

• WB

Supplier Data

Western blot - Anti-HCoV-OC43 Nucleoprotein antibody [abd384] - BSA and Azide free (AB309965)

This data was developed using ab309964, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) was used at 1 : 5000 dilution.

All lanes:

Western blot - Anti-HCoV-OC43 Nucleoprotein antibody [abd384] (<a href='/en-us/products/primary-antibodies/hcov-oc43-nucleoprotein-antibody-abd384-ab309964'>ab309964</a>) at 1/1000 dilution

All lanes:

His-tagged HCoV-OC43 Nucleoprotein recombinant protein at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Human IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-human-igg-h-l-hrp-ab6858'>ab6858</a>) at 1/5000 dilution

Observed band size: 65 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-HCoV-OC43 Nucleoprotein antibody [abd384] - BSA and Azide free (AB309965)

This data was developed using ab309964, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) was used at 1 : 200000 dilution. In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) was used at 1 : 5000 dilution.

All lanes:

Western blot - Anti-HCoV-OC43 Nucleoprotein antibody [abd384] (<a href='/en-us/products/primary-antibodies/hcov-oc43-nucleoprotein-antibody-abd384-ab309964'>ab309964</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cells) transfected with an empty vector containing a His-tag, whole cell lysate at 4 µg

Lane 2:

293T (human embryonic kidney epithelial cells) transfected with HCoV-OC43 Nucleoprotein expression vector containing a His-tag, whole cell lysate at 4 µg

Secondary

All lanes:

Western blot - Goat Anti-Human IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-human-igg-h-l-hrp-ab6858'>ab6858</a>) at 1/5000 dilution

Observed band size: 65 kDa

false

Exposure time: 1s