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AB241949

Anti-HCP1/PCFT antibody

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(1 Publication)

Rabbit Polyclonal HCP1/PCFT antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SLC46A1 aa 200 to C-terminus.

View Alternative Names

G21, HCP1, PCFT, SLC46A1, Proton-coupled folate transporter, HsPCFT, hPCFT, Heme carrier protein 1, PCFT/HCP1, Solute carrier family 46 member 1

2 Images
Immunoprecipitation - Anti-HCP1/PCFT antibody (AB241949)
  • IP

Supplier Data

Immunoprecipitation - Anti-HCP1/PCFT antibody (AB241949)

HCP1/PCFT was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.

ab241949 used for IP at 6 μg per reaction. For WB 0.1 μg/ml.

Lane 1 : ab241949 IP in HeLa whole cell lysate.
Lane 2 : Control IgG.

Chemiluminescence detection : 30 seconds.

All lanes:

Immunoprecipitation - Anti-HCP1/PCFT antibody (ab241949)

Predicted band size: 50 kDa

false

Western blot - Anti-HCP1/PCFT antibody (AB241949)
  • WB

Supplier Data

Western blot - Anti-HCP1/PCFT antibody (AB241949)

Prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-HCP1/PCFT antibody (ab241949) at 0.1 µg/mL

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Predicted band size: 50 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human SLC46A1 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q96NT5

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antibody was affinity purified using an epitope specific to HCP1/PCFT immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HCP1/PCFT also known as HCP1 HCP1 form or HCP-1 is a proton-coupled folate transporter. The protein has a mass of approximately 50 kDa and is expressed mainly in the duodenum and jejunum of the small intestine. It plays a central role in the absorption of dietary folate and folate metabolites. The presence of HCP1/PCFT is also noted in the liver and other tissues serving functions related to folate-dependent metabolic processes.
Biological function summary

HCP1/PCFT functions as a transporter that facilitates the absorption of folate across intestinal epithelial cells. It interacts with FOLR1 forming a functional complex contributing to folate uptake. This activity is essential for maintaining folate levels within cells influencing cell division and growth. The unique transport mechanism of HCP1/PCFT is pH-dependent operating optimally under acidic conditions found in the proximal intestine.

Pathways

HCP1/PCFT is involved in the folate and one-carbon metabolism pathways. It works closely with other transporters like RFC (Reduced Folate Carrier) and proteins such as DHFR (Dihydrofolate Reductase) to ensure efficient folate utilization. In the metabolic pathway these interactions are important for purine and pyrimidine synthesis which are necessary for DNA replication and repair.

Alterations in HCP1/PCFT function are linked to hereditary folate malabsorption and certain types of anemia. Impaired HCP1/PCFT activity can cause systemic folate deficiency leading to developmental issues and hematological abnormalities. The transporter is also connected to cancer as folate metabolism impacts tumor growth involving proteins like TYMS (Thymidylate Synthase) in its metabolic network.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Proton-coupled folate symporter that mediates folate absorption using an H(+) gradient as a driving force (PubMed : 17129779, PubMed : 17446347, PubMed : 17475902, PubMed : 19389703, PubMed : 19762432, PubMed : 25504888, PubMed : 29344585, PubMed : 30858177, PubMed : 31494288, PubMed : 31792273, PubMed : 32893190, PubMed : 34619546). Involved in the intestinal absorption of folates at the brush-border membrane of the proximal jejunum, and the transport from blood to cerebrospinal fluid across the choroid plexus (PubMed : 17129779, PubMed : 17446347, PubMed : 17475902, PubMed : 19389703, PubMed : 25504888, PubMed : 29344585, PubMed : 30858177, PubMed : 31494288, PubMed : 32893190). Functions at acidic pH via alternate outward- and inward-open conformation states (PubMed : 32893190, PubMed : 34040256). Protonation of residues in the outward open state primes the protein for transport (PubMed : 34040256). Binding of folate promotes breaking of salt bridge network and subsequent closure of the extracellular gate, leading to the inward-open state and release of protons and folate (PubMed : 34040256). Also able to transport antifolate drugs, such as methotrexate and pemetrexed, which are established treatments for cancer and autoimmune diseases (PubMed : 18524888, PubMed : 19762432, PubMed : 22345511, PubMed : 25608532, PubMed : 28802835, PubMed : 29326243, PubMed : 34040256, PubMed : 34619546). Involved in FOLR1-mediated endocytosis by serving as a route of export of folates from acidified endosomes (PubMed : 19074442). Also acts as a lower-affinity, pH-independent heme carrier protein and constitutes the main importer of heme in the intestine (PubMed : 17156779). Imports heme in the retina and retinal pigment epithelium, in neurons of the hippocampus, in hepatocytes and in the renal epithelial cells (PubMed : 32621820). Hence, participates in the trafficking of heme and increases intracellular iron content (PubMed : 32621820).. Isoform 2. Inactive isoform which is not able to mediate proton-coupled folate transport.
See full target information SLC46A1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature structural & molecular biology 31:747-751 PubMed38467876

2024

Structural basis of human U5 snRNP late biogenesis and recycling.

Applications

Unspecified application

Species

Unspecified reactive species

Daria Riabov Bassat,Supapat Visanpattanasin,Matthias K Vorländer,Laura Fin,Alexander W Phillips,Clemens Plaschka
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