AB35586

Anti-HCV subtype 1b NS5B antibody

Name: Anti-HCV subtype 1b NS5B antibody (ab35586) | Abcam
Brand: Abcam Limited
SKU: AB35586
Price: 490 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(18 Publications)

Rabbit Polyclonal POLG antibody. Suitable for ELISA, WB and reacts with Hepatitis C virus samples. Cited in 18 publications.

View Alternative Names

Hepacivirin, NS3P, POLG_HCVBK, Serine protease NS3

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Hepatitis C virus

Applications

ELISA, WB

applications

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HCV subtype 1b NS5B also known as nonstructural protein 5B is an RNA-dependent RNA polymerase (RdRp) important for Hepatitis C virus replication. This enzyme is an important component of the HCV replication complex facilitating viral RNA synthesis. It has a molecular mass of approximately 66 kDa. NS5B is expressed in infected liver cells where it remains membrane-associated due to its C-terminal tail. Antibodies against HCV NS5B such as anti-HCV are often utilized in research for detection and analysis including HCV Western blot applications.

Biological function summary

This enzyme plays a critical role in the synthesis of viral RNA by using viral RNA as a template. NS5B is not part of a classical protein complex but interacts with other viral proteins in the replication complex including NS3 and NS5A to carry out RNA replication and produce progeny virus. Anti-HCV antibodies can aid in studying these interactions. Enzyme inhibitors targeting NS5B are a major focus in HCV therapeutic development efforts.

Pathways

NS5B participates in the life cycle of HCV specifically the replication phase. The replication of HCV involves multiple stages and NS5B's role is integral in the RNA synthesis step. It interacts with the NS5A protein which modulates RNA replication. The interplay between NS5B and NS5A represents a significant point in the HCV replication pathway influencing viral loads and laboratory studies often use anti-HCV and anti-HCV negative controls in mechanistic research.

HCV subtype 1b NS5B is central in chronic Hepatitis C infection and liver diseases such as cirrhosis and hepatocellular carcinoma. Interference with NS5B's RNA polymerase activity is a proven strategy in antiviral therapy making it a target for drugs like polymerase inhibitors. Its connection to NS5A which also supports viral replication corroborates its relevance in HCV pathology and targeted therapies often include combination treatments that address both proteins to improve patient outcomes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information HCV subtype 1b NS5B

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Publications (18)

Recent publications for all applications. Explore the full list and refine your search

PloS one 19:e0303265 PubMed38739590

2024

Immortalized hepatocyte-like cells: A competent hepatocyte model for studying clinical HCV isolate infection.

Applications

Unspecified application

Species

Unspecified reactive species

Yongyut Pewkliang,Piyanoot Thongsri,Phichaya Suthivanich,Nipa Thongbaiphet,Jiraporn Keatkla,Ekawat Pasomsub,Usanarat Anurathapan,Suparerk Borwornpinyo,Adisak Wongkajornsilp,Suradej Hongeng,Khanit Sa-Ngiamsuntorn

International journal of molecular sciences 22: PubMed33925399

2021

Pre-Senescence Induction in Hepatoma Cells Favors Hepatitis C Virus Replication and Can Be Used in Exploring Antiviral Potential of Histone Deacetylase Inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Alsu Z Malikova,Anastasia S Shcherbakova,Konstantin A Konduktorov,Anastasia S Zemskaya,Alexandra A Dalina,Vladimir I Popenko,Olga G Leonova,Alexei V Morozov,Nikolay N Kurochkin,Olga A Smirnova,Sergey N Kochetkov,Maxim V Kozlov

Biophysical journal 116:1328-1339 PubMed30879645

2019

A Nondimensional Model Reveals Alterations in Nuclear Mechanics upon Hepatitis C Virus Replication.

Applications

Unspecified application

Species

Unspecified reactive species

Sreenath Balakrishnan,Suma S Mathad,Geetika Sharma,Shilpa R Raju,Uma B Reddy,Saumitra Das,G K Ananthasuresh

Scientific reports 7:11250 PubMed28900156

2017

Dual Roles of Two Isoforms of Autophagy-related Gene ATG10 in HCV-Subgenomic replicon Mediated Autophagy Flux and Innate Immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Qiong Zhao,Zhan-Ying Hu,Jing-Pu Zhang,Jian-Dong Jiang,Yuan-Yuan Ma,Jian-Rui Li,Zong-Gen Peng,Jin-Hua Chen

Journal of virology 90:6022-6035 PubMed27099311

2016

NOD1 Participates in the Innate Immune Response Triggered by Hepatitis C Virus Polymerase.

Applications

Unspecified application

Species

Unspecified reactive species

Serena Vegna,Damien Gregoire,Marie Moreau,Patrice Lassus,David Durantel,Eric Assenat,Urszula Hibner,Yannick Simonin

Antimicrobial agents and chemotherapy 60:3540-50 PubMed27021315

2016

Hepatitis C Virus RNA-Dependent RNA Polymerase Interacts with the Akt/PKB Kinase and Induces Its Subcellular Relocalization.

Applications

Unspecified application

Species

Unspecified reactive species

María Llanos Valero,Rosario Sabariegos,Francisco J Cimas,Celia Perales,Esteban Domingo,Ricardo Sánchez-Prieto,Antonio Mas

Journal of virology 89:11356-71 PubMed26339049

2015

HuR Displaces Polypyrimidine Tract Binding Protein To Facilitate La Binding to the 3' Untranslated Region and Enhances Hepatitis C Virus Replication.

Applications

Species

Human

Shivaprasad Shwetha,Anuj Kumar,Ranajoy Mullick,Deeptha Vasudevan,Nilanjan Mukherjee,Saumitra Das

International journal of molecular medicine 35:791-7 PubMed25572289

2015

A zebrafish model for subgenomic hepatitis C virus replication.

Applications

Unspecified application

Species

Unspecified reactive species

Cun-Bao Ding,Ye Zhao,Jing-Pu Zhang,Zong-Gen Peng,Dan-Qing Song,Jian-Dong Jiang

Antimicrobial agents and chemotherapy 59:85-95 PubMed25313218

2014

The bioactive lipid 4-hydroxyphenyl retinamide inhibits flavivirus replication.

Applications

Unspecified application

Species

Unspecified reactive species

Margot Carocci,Stephen M Hinshaw,Mary A Rodgers,Valerie A Villareal,Dominique J Burri,Rajendra Pilankatta,Natalya P Maharaj,Michaela U Gack,Eric J Stavale,Kelly L Warfield,Priscilla L Yang

PloS one 8:e82527 PubMed24358200

2013

Isolation and characterization of highly replicable hepatitis C virus genotype 1a strain HCV-RMT.

Applications

Species

Unspecified reactive species

Masaaki Arai,Yuko Tokunaga,Asako Takagi,Yoshimi Tobita,Yuichi Hirata,Yuji Ishida,Chise Tateno,Michinori Kohara

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HCV subtype 1b NS5B antibody

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Alternative Products

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Assay Kits

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (18)

Product promise