Anti-HDAC1 antibody - Nuclear Loading Control

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-HDAC1 antibody - Nuclear Loading Control (AB53091)

ICC/IF image of ab53091 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53091, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody - Nuclear Loading Control (AB53091)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labeling HDAC1 with ab53091. The image on the right is blocked with the synthesized peptide.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody - Nuclear Loading Control (AB53091)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ab53091 at 1/50 dilution, in the presence (right) or absence (left) of blocking peptide.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HDAC1 antibody - Nuclear Loading Control (AB53091)

Immunocytochemistry/ Immunofluorescence analysis of NIH/3T3 cells labeling HDAC1 with ab53091. The image on the right is blocked with the synthesized peptide.

• ICC/IF

AbReview30349****

Immunocytochemistry/ Immunofluorescence - Anti-HDAC1 antibody - Nuclear Loading Control (AB53091)

Immunocytochemistry/ Immunofluorescence analysis of mouse RAW 264.7 cells labeling HDAC1 with ab53091 at 1/150 dilution. The cells were fixed with paraformaldehyde and permeabilized with 0.1% Triton-X100 in 2% BSA for 15min. Blocking of the cells was done with 2% BSA for 1 hour at 4°C, followed by staining with ab53091 at 1/150 in 2% BSA in PBS for 16 hours at 4°C. An Alexa Fluor^® 488 conjugated chicken anti-rabbit IgG (H+L) secondary antibody was used at 1/1000 dilution.

This image is courtesy of an abreview submitted by Dr Mahesh Shivananjappa.

• WB

Supplier Data

Western blot - Anti-HDAC1 antibody - Nuclear Loading Control (AB53091)

All lanes:

Western blot - Anti-HDAC1 antibody - Nuclear Loading Control (ab53091)

Lane 1:

NIH/3T3 cell lysates

Lane 2:

NIH/3T3 cell lysates blocked with synthesized peptide

Predicted band size: 55 kDa

false

• WB

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Western blot - Anti-HDAC1 antibody - Nuclear Loading Control (AB53091)

All lanes:

Western blot - Anti-HDAC1 antibody - Nuclear Loading Control (ab53091) at 1/300 dilution

Lane 1:

NIH/3T3 cell extracts

Lane 2:

NIH/3T3 cell extracts with blocking peptide

Predicted band size: 55 kDa

Observed band size: 60 kDa

false