Rabbit Polyclonal HDAC1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 21 publications. Immunogen corresponding to Synthetic Peptide within Human HDAC1.
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Publications
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- Nucleic acids research 50:10343-103592022The transcriptional coactivator Eya1 exerts transcriptional repressive activity by interacting with REST corepressors and REST-binding sequences to maintain nephron progenitor identity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun Li,Chunming Cheng,Jinshu Xu,Ting Zhang,Bengu Tokat,Georgia Dolios,Aarthi Ramakrishnan,Li Shen,Rong Wang,Pin-Xian XuPubMed 36130284
- International journal of molecular medicine 49:2021HDAC1 promotes the migration of human myeloma cells via regulation of the lncRNA/Slug axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLisha Zheng,Ang Zhang,Jishan Liu,Min Liu,Yikun ZhangPubMed 34738621
- Dose-response : a publication of International Hormesis Society 19:155932582110012512021Moscatilin Suppresses the Breast Cancer Both In Vitro and In Vivo by Inhibiting HDAC3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenjie Su,Lianfu Zeng,Weida ChenPubMed 33795998
- Brazilian journal of medical and biological resear 54:e101072020Rapid and long-lasting antidepressant-like effects of ketamine and their relationship with the expression of brain enzymes, BDNF, and astrocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesG S B Viana et. al.PubMed 33331415
- Cell death & disease 11:10432020LncRNA kcnq1ot1 promotes lipid accumulation and accelerates atherosclerosis via functioning as a ceRNA through the miR-452-3p/HDAC3/ABCA1 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiao-Hua Yu et. al.PubMed 33293505
- Experimental and therapeutic medicine 20:352020Epigenetic modulation of the MAPK pathway prevents isoflurane-induced neuronal apoptosis and cognitive decline in aged rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLei Huang et. al.PubMed 32952626
- Gene 754:1448512020The histone deacetylase HDAC1 activates HIF1α/VEGFA signal pathway in colorectal cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCheng Chen et. al.PubMed 32525044
- Cancer management and research 12:497-5092020Procyanidin Compound (PC) Suppresses Lipopolysaccharide-Induced Cervical Cancer Cell Proliferation Through Blocking the TLR4/NF-κB Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaiyan Yang et. al.PubMed 32158256
- Aging 11:9778-97932019Ubiquitin specific peptidase 5 promotes ovarian cancer cell proliferation through deubiquitinating HDAC2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanhua Du et. al.PubMed 31727867
- Anticancer research 39:5473-54812019Disruption of Epigenetic Silencing in Human Colon Cancer Cells Lines Utilizing a Novel Supercritical CO Extract of Neem Leaf ().Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhifang Qiu,Kristina Andrijauskaite,Jay Morris,Michael J WargovichPubMed 31570441
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human HDAC1. The exact immunogen used to generate this antibody is proprietary information. Database link Q13547
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Expected | Tested | Tested |
| Mouse | Tested | Expected | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/300.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
Histone deacetylase that catalyzes the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) (PubMed:16762839, PubMed:17704056, PubMed:28497810). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events (PubMed:16762839, PubMed:17704056). Histone deacetylases act via the formation of large multiprotein complexes (PubMed:16762839, PubMed:17704056). Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin (PubMed:16428440, PubMed:28977666). As part of the SIN3B complex is recruited downstream of the constitutively active genes transcriptional start sites through interaction with histones and mitigates histone acetylation and RNA polymerase II progression within transcribed regions contributing to the regulation of transcription (PubMed:21041482). Also functions as a deacetylase for non-histone targets, such as NR1D2, RELA, SP1, SP3, STAT3 and TSHZ3 (PubMed:12837748, PubMed:16285960, PubMed:16478997, PubMed:17996965, PubMed:19343227). Deacetylates SP proteins, SP1 and SP3, and regulates their function (PubMed:12837748, PubMed:16478997). Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons (PubMed:19081374). Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation (PubMed:19081374). Deacetylates TSHZ3 and regulates its transcriptional repressor activity (PubMed:19343227). Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B (PubMed:17000776). Deacetylates NR1D2 and abrogates the effect of KAT5-mediated relieving of NR1D2 transcription repression activity (PubMed:17996965). Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development (By similarity). Involved in CIART-mediated transcriptional repression of the circadian transcriptional activator: CLOCK-BMAL1 heterodimer (By similarity). Required for the transcriptional repression of circadian target genes, such as PER1, mediated by the large PER complex or CRY1 through histone deacetylation (By similarity). In addition to protein deacetylase activity, also has protein-lysine deacylase activity: acts as a protein decrotonylase and delactylase by mediating decrotonylation ((2E)-butenoyl) and delactylation (lactoyl) of histones, respectively (PubMed:28497810, PubMed:35044827).
Alternative names
RPD3L1, HDAC1, Histone deacetylase 1, HD1, Protein deacetylase HDAC1, Protein deacylase HDAC1
Recommended products
Rabbit Polyclonal HDAC1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 21 publications. Immunogen corresponding to Synthetic Peptide within Human HDAC1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human HDAC1. The exact immunogen used to generate this antibody is proprietary information. Database link Q13547
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab53091 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
HDAC1 also known as Histone Deacetylase 1 is a member of the histone deacetylase family with a molecular weight of approximately 55 kDa. Mechanically HDAC1 removes acetyl groups from lysine residues on histone proteins an action known as histone deacetylation. This process causes chromatin structure to become more compact which leads to transcriptional repression. HDAC1 is broadly expressed in various tissues particularly in the brain heart and kidneys and is vital for cellular development and differentiation.
- Biological function summary
The enzymatic activity of histone deacetylase effectively controls gene expression. HDAC1 participates as a part of the multiprotein complexes including SIN3 and NuRD which play vital roles in the regulation of transcription. By altering the acetylation state of histones HDAC1 influences chromatin remodeling thereby affecting the accessibility of transcription factors to DNA and controlling genes necessary for cell cycle progression and proliferation.
- Pathways
The function of HDAC1 fits into the regulation of the cell cycle and apoptosis pathways. In the cell cycle pathway HDAC1 interacts with other histone deacetylases (HDACs) and plays a role in controlling the progression of the cell division. The interplay between HDAC1 and proteins such as p53 further showcases its regulatory activity in apoptosis ensuring cell survival or programmed cell death when necessary.
- Associated diseases and disorders
HDAC1 shows significant relevance to cancer and neurodegenerative diseases. In cancer the overexpression or abnormal regulation of HDAC1 can lead to uncontrolled cell proliferation often linked to the silencing of tumor suppressor genes. Within neurodegenerative conditions HDAC1-related disturbances in gene expression may result in impaired neuronal function and survival. The involvement of HDAC1 with proteins such as p53 and other HDACs illustrates its impact on complex disease mechanisms making it a critical target for therapeutic interventions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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7 product images
Western blot - Anti-HDAC1 antibody - Nuclear Loading Control (ab53091)
HDAC1 Western blot staining of NIH/3T3 cell extracts using rabbit Anti-HDAC1 antibody
All lanes: Western blot - Anti-HDAC1 antibody - Nuclear Loading Control (ab53091) at 1/300 dilution
Lane 1: NIH/3T3 cell extracts
Lane 2: NIH/3T3 cell extracts with blocking peptide
Predicted band size: 55 kDa
Observed band size: 60 kDa
Immunocytochemistry/ Immunofluorescence - Anti-HDAC1 antibody - Nuclear Loading Control (ab53091)
HDAC1 Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-HDAC1 antibody
ICC/IF image of ab53091 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53091, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody (ab53091)
Immunohistochemical analysis of paraffin-embedded
human lung carcinoma tissue using ab53091 at 1/50 dilution, in the presence (right) or absence (left) of blocking peptide.
This image is courtesy of a customer review submitted by Dr Mahesh Shivananjappa.Immunocytochemistry/ Immunofluorescence - Anti-HDAC1 antibody - Nuclear Loading Control (ab53091)
HDAC1 Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-HDAC1 antibody
Immunocytochemistry/ Immunofluorescence analysis of mouse RAW 264.7 cells labeling HDAC1 with ab53091 at 1/150 dilution. The cells were fixed with paraformaldehyde and permeabilized with 0.1% Triton-X100 in 2% BSA for 15min. Blocking of the cells was done with 2% BSA for 1 hour at 4°C, followed by staining with ab53091 at 1/150 in 2% BSA in PBS for 16 hours at 4°C. An Alexa Fluor® 488 conjugated chicken anti-rabbit IgG (H+L) secondary antibody was used at 1/1000 dilution.
Western blot - Anti-HDAC1 antibody - Nuclear Loading Control (ab53091)
HDAC1 Western blot staining using rabbit Anti-HDAC1 antibody
All lanes: Western blot - Anti-HDAC1 antibody - Nuclear Loading Control (ab53091)
Lane 1: NIH/3T3 cell lysates
Lane 2: NIH/3T3 cell lysates blocked with synthesized peptide
Predicted band size: 55 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody - Nuclear Loading Control (ab53091)
HDAC1 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-HDAC1 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labeling HDAC1 with ab53091. The image on the right is blocked with the synthesized peptide.
Immunocytochemistry/ Immunofluorescence - Anti-HDAC1 antibody (ab53091)
Immunocytochemistry/ Immunofluorescence analysis of NIH/3T3 cells labeling HDAC1 with ab53091. The image on the right is blocked with the synthesized peptide.
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