Rabbit Polyclonal HDAC1 antibody. Suitable for ICC/IF, IP, WB and reacts with Human, Chinese hamster, Mouse, Rat samples. Cited in 239 publications. Immunogen corresponding to Synthetic Peptide within Human HDAC1 aa 450 to C-terminus conjugated to Keyhole Limpet Haemocyanin.
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- Cancer research communications 3:1716-17302023Demethylation of EHMT1/GLP Protein Reprograms Its Transcriptional Activity and Promotes Prostate Cancer Progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnna Besschetnova,Wanting Han,Mingyu Liu,Yanfei Gao,Muqing Li,Zifeng Wang,Maryam Labaf,Susan Patalano,Kavita Venkataramani,Rachel E Muriph,Jill A Macoska,Kellee R Siegfried,Jason Evans,Steven P Balk,Shuai Gao,Dong Han,Changmeng CaiPubMed 37663929
- Nature communications 14:29832023Hyperphosphorylated PTEN exerts oncogenic properties.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJanine H van Ree,Karthik B Jeganathan,Raul O Fierro Velasco,Cheng Zhang,Ismail Can,Masakazu Hamada,Hu Li,Darren J Baker,Jan M van DeursenPubMed 37225693
- Nature communications 14:15212023Acute liver steatosis translationally controls the epigenetic regulator MIER1 to promote liver regeneration in a study with male mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanhao Chen,Lanlan Chen,Xiaoshan Wu,Yongxu Zhao,Yuchen Wang,Dacheng Jiang,Xiaojian Liu,Tingting Zhou,Shuang Li,Yuda Wei,Yan Liu,Cheng Hu,Ben Zhou,Jun Qin,Hao Ying,Qiurong DingPubMed 36934083
- Nature communications 13:41182022Hypoxia induces HIF1α-dependent epigenetic vulnerability in triple negative breast cancer to confer immune effector dysfunction and resistance to anti-PD-1 immunotherapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShijun Ma,Yue Zhao,Wee Chyan Lee,Li-Teng Ong,Puay Leng Lee,Zemin Jiang,Gokce Oguz,Zhitong Niu,Min Liu,Jian Yuan Goh,Wenyu Wang,Matias A Bustos,Sidse Ehmsen,Adaikalavan Ramasamy,Dave S B Hoon,Henrik J Ditzel,Ern Yu Tan,Qingfeng Chen,Qiang YuPubMed 35840558
- The Journal of neuroscience : the official journal of the Society for Neuroscience 42:4594-46042022CREB Inactivation by HDAC1/PP1γ Contributes to Dopaminergic Neurodegeneration in Parkinson's Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoyi Xu,Xin He,Zeyan Zhang,Yanyi Chen,Junyu Li,Shanshan Ma,Qiaoying Huang,Mingtao LiPubMed 35501151
- iScience 25:1041962022Gene repression through epigenetic modulation by PPARA enhances hepatocellular proliferation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaisuke Aibara,Shogo Takahashi,Tomoki Yagai,Donghwan Kim,Chad N Brocker,Moshe Levi,Kimihiko Matsusue,Frank J GonzalezPubMed 35479397
- Nature communications 13:15502022Unveiling RCOR1 as a rheostat at transcriptionally permissive chromatin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCarlos Rivera,Hun-Goo Lee,Anna Lappala,Danni Wang,Verónica Noches,Montserrat Olivares-Costa,Marcela Sjöberg-Herrera,Jeannie T Lee,María Estela AndrésPubMed 35322029
- Cell genomics 2:2022CASCADE: high-throughput characterization of regulatory complex binding altered by non-coding variants.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDavid Bray,Heather Hook,Rose Zhao,Jessica L Keenan,Ashley Penvose,Yemi Osayame,Nima Mohaghegh,Xiaoting Chen,Sreeja Parameswaran,Leah C Kottyan,Matthew T Weirauch,Trevor SiggersPubMed 35252945
- Molecular cell 82:833-851.e112022HOTTIP-dependent R-loop formation regulates CTCF boundary activity and TAD integrity in leukemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuacheng Luo,Ganqian Zhu,Melanie A Eshelman,Tsz Kan Fung,Qian Lai,Fei Wang,Bernd B Zeisig,Julia Lesperance,Xiaoyan Ma,Shi Chen,Nicholas Cesari,Christopher Cogle,Baoan Chen,Bing Xu,Feng-Chun Yang,Chi Wai Eric So,Yi Qiu,Mingjiang Xu,Suming HuangPubMed 35180428
- Nature communications 13:8082022GLI transcriptional repression is inert prior to Hedgehog pathway activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRachel K Lex,Weiqiang Zhou,Zhicheng Ji,Kristin N Falkenstein,Kaleigh E Schuler,Kathryn E Windsor,Joseph D Kim,Hongkai Ji,Steven A VokesPubMed 35145123
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human HDAC1 aa 450 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q13547
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Reactivity data
Select an application| ICC/IF | IP | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Expected | Tested |
| Rat | Expected | Expected | Tested |
| Chinese hamster | Expected | Expected | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Chinese hamster, Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Use 5 -10 μL antibody per reaction |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Chinese hamster, Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Chinese hamster | Dilution info 1/2000 - 1/20000 | Notes - |
Species Mouse | Dilution info 1/2000 - 1/20000 | Notes - |
Species Rat | Dilution info 1/2000 - 1/20000 | Notes - |
Species Human | Dilution info 1/2000 - 1/20000 | Notes - |
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Target data
Function
Histone deacetylase that catalyzes the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) (PubMed:16762839, PubMed:17704056, PubMed:28497810). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events (PubMed:16762839, PubMed:17704056). Histone deacetylases act via the formation of large multiprotein complexes (PubMed:16762839, PubMed:17704056). Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin (PubMed:16428440, PubMed:28977666). As part of the SIN3B complex is recruited downstream of the constitutively active genes transcriptional start sites through interaction with histones and mitigates histone acetylation and RNA polymerase II progression within transcribed regions contributing to the regulation of transcription (PubMed:21041482). Also functions as a deacetylase for non-histone targets, such as NR1D2, RELA, SP1, SP3, STAT3 and TSHZ3 (PubMed:12837748, PubMed:16285960, PubMed:16478997, PubMed:17996965, PubMed:19343227). Deacetylates SP proteins, SP1 and SP3, and regulates their function (PubMed:12837748, PubMed:16478997). Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons (PubMed:19081374). Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation (PubMed:19081374). Deacetylates TSHZ3 and regulates its transcriptional repressor activity (PubMed:19343227). Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B (PubMed:17000776). Deacetylates NR1D2 and abrogates the effect of KAT5-mediated relieving of NR1D2 transcription repression activity (PubMed:17996965). Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development (By similarity). Involved in CIART-mediated transcriptional repression of the circadian transcriptional activator: CLOCK-BMAL1 heterodimer (By similarity). Required for the transcriptional repression of circadian target genes, such as PER1, mediated by the large PER complex or CRY1 through histone deacetylation (By similarity). In addition to protein deacetylase activity, also has protein-lysine deacylase activity: acts as a protein decrotonylase and delactylase by mediating decrotonylation ((2E)-butenoyl) and delactylation (lactoyl) of histones, respectively (PubMed:28497810, PubMed:35044827).
Alternative names
RPD3L1, HDAC1, Histone deacetylase 1, HD1, Protein deacetylase HDAC1, Protein deacylase HDAC1
Recommended products
Rabbit Polyclonal HDAC1 antibody. Suitable for ICC/IF, IP, WB and reacts with Human, Chinese hamster, Mouse, Rat samples. Cited in 239 publications. Immunogen corresponding to Synthetic Peptide within Human HDAC1 aa 450 to C-terminus conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human HDAC1 aa 450 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q13547
- Purity
- IgG fraction
- Concentration
- Purification notes
Whole antiserum is fractionated and further purified by anion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
HDAC1 also known as Histone Deacetylase 1 is a member of the histone deacetylase family with a molecular weight of approximately 55 kDa. Mechanically HDAC1 removes acetyl groups from lysine residues on histone proteins an action known as histone deacetylation. This process causes chromatin structure to become more compact which leads to transcriptional repression. HDAC1 is broadly expressed in various tissues particularly in the brain heart and kidneys and is vital for cellular development and differentiation.
- Biological function summary
The enzymatic activity of histone deacetylase effectively controls gene expression. HDAC1 participates as a part of the multiprotein complexes including SIN3 and NuRD which play vital roles in the regulation of transcription. By altering the acetylation state of histones HDAC1 influences chromatin remodeling thereby affecting the accessibility of transcription factors to DNA and controlling genes necessary for cell cycle progression and proliferation.
- Pathways
The function of HDAC1 fits into the regulation of the cell cycle and apoptosis pathways. In the cell cycle pathway HDAC1 interacts with other histone deacetylases (HDACs) and plays a role in controlling the progression of the cell division. The interplay between HDAC1 and proteins such as p53 further showcases its regulatory activity in apoptosis ensuring cell survival or programmed cell death when necessary.
- Associated diseases and disorders
HDAC1 shows significant relevance to cancer and neurodegenerative diseases. In cancer the overexpression or abnormal regulation of HDAC1 can lead to uncontrolled cell proliferation often linked to the silencing of tumor suppressor genes. Within neurodegenerative conditions HDAC1-related disturbances in gene expression may result in impaired neuronal function and survival. The involvement of HDAC1 with proteins such as p53 and other HDACs illustrates its impact on complex disease mechanisms making it a critical target for therapeutic interventions.
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Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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7 product images
This image is courtesy of an anonymous customer reviewWestern blot - Anti-HDAC1 antibody - Nuclear Loading Control (ab7028)
HDAC1 Western blot staining using rabbit Anti-HDAC1 antibody
All lanes: Western blot - Anti-HDAC1 antibody - Nuclear Loading Control (ab7028) at 1/1000 dilution
Lane 1: Whole cell lysate from human HEK293 cell line at 20 µg
Lane 2: Whole cell lysate from human HEK293 cell line treated with HDAC1 gene silencing shRNA at 20 µg
SecondaryAll lanes: HRP-conjugated goat anti-rabbit Ig at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 55 kDa
Exposure time: 10s
Immunoprecipitation - Anti-HDAC1 antibody - Nuclear Loading Control (ab7028)
HDAC1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with ab7028.
Lane 1: 5 μl ab7028 in HeLa whole cell lysate.
Lane 2: 10 μl ab7028 in HeLa whole cell lysate.
Lane 3: 10 μl control antibody in HeLa whole cell lysate.
All lanes: Immunoprecipitation - Anti-HDAC1 antibody - Nuclear Loading Control (ab7028)
Predicted band size: 55 kDa
Western blot - Anti-HDAC1 antibody - Nuclear Loading Control (ab7028)
HDAC1 Western blot staining using rabbit Anti-HDAC1 antibody
All lanes: Western blot - Anti-HDAC1 antibody - Nuclear Loading Control (ab7028) at 1/10000 dilution
Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2: HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3: K562 (human chronic myelogenous leukemia cell line from bone marrow ) cell lysate
Lane 4: CHO (Chinese hamster ovary cell line) cell lysate
Lane 5: NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
Lane 6: PC-12 (rat adrenal gland pheochromocytoma cell line) cell lysate
SecondaryAll lanes: Goat Anti-Rabbit HRP
Predicted band size: 55 kDa
Hanigan, T.W. et al Send to PLoS One. 2017 Oct 18;12(10):e0186620. doi: 10.1371/journal.pone.0186620. eCollection 2017 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Immunocytochemistry/ Immunofluorescence - Anti-HDAC1 antibody (ab7028)
MCF-7 cells were treated with indicated concentrations of panobinostat (optical sections A-D, respectively) or trichostatin A (optical sections E-H, respectively) for 12 hours, fixed, permeabilized and optical sections were obtained by laser scanning confocal microscopy. Fluorescence signal for HDAC1 is shown in green (left panels), DAPI staining is shown in blue (middle panels), and merged optical sections are shown in the right panels. Colocalization analysis of HDAC1 fluorescence signal and the DAPI stain signal was performed with JACoP (ImageJ).
HDAC1 was detected with ab7028. cells were ficed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
(After Figure 3 of Hanigan et al).
Immunocytochemistry/ Immunofluorescence - Anti-HDAC1 antibody (ab7028)
Immunofluorescence analysis of methanol fixed HeLa cells with ab7028 labelling HDAC1 at 1/200 dilution. The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate.
Immunoprecipitation - Anti-HDAC1 antibody - Nuclear Loading Control (ab7028)
HDAC1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5μg of Rabbit polyclonal to HDAC1 and 50μl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40μl SDS loading buffer and incubated for 10min at 70oC; 10μl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab7028.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (HRP) ab99697).
Band: 60ka: HDAC1.All lanes: Immunoprecipitation - Anti-HDAC1 antibody - Nuclear Loading Control (ab7028)
Predicted band size: 55 kDa
This image is courtesy of an anonymous customer reviewWestern blot - Anti-HDAC1 antibody - Nuclear Loading Control (ab7028)
HDAC1 Western blot staining of Human HuH-7 whole cell lysate using rabbit Anti-HDAC1 antibody
Blocked with 5% milk for 1 hour at 20°C
All lanes: Western blot - Anti-HDAC1 antibody - Nuclear Loading Control (ab7028) at 1/2000 dilution
All lanes: Human HuH-7 whole cell lysate at 15 µg
SecondaryAll lanes: HRP conjugated Sheet anti-rabbit IgG polyclonal at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 55 kDa
Exposure time: 20s
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