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Knockout Tested Rabbit Recombinant Multiclonal HDAC1 antibody. Suitable for Flow Cyt (Intra), IHC-P, IP, ICC/IF, WB and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-HDAC1 + HDAC2 + HDAC3 + HDAC8 antibody [RM1172] (AB319059), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-HDAC1 + HDAC2 + HDAC3 + HDAC8 antibody [RM1172] (AB319059), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-HDAC1 + HDAC2 + HDAC3 + HDAC8 antibody [RM1172] (AB319059), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-HDAC1 + HDAC2 + HDAC3 + HDAC8 antibody [RM1172] (AB319059), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-HDAC1 + HDAC2 + HDAC3 + HDAC8 antibody [RM1172] (AB319059), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Multiclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt (Intra)IHC-PIPICC/IFWB
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Tested
Tested
Not recommended
Tested
Tested
Rat
Tested
Tested
Not recommended
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/500

Notes

-

Species

Mouse

Dilution info

1/500

Notes

-

Species

Rat

Dilution info

1/500

Notes

-

Tested
Tested

Species

Human

Dilution info

1/5000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

1/5000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/5000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

1/30

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Histone deacetylase that catalyzes the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) (PubMed:16762839, PubMed:17704056, PubMed:28497810). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events (PubMed:16762839, PubMed:17704056). Histone deacetylases act via the formation of large multiprotein complexes (PubMed:16762839, PubMed:17704056). Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin (PubMed:16428440, PubMed:28977666). As part of the SIN3B complex is recruited downstream of the constitutively active genes transcriptional start sites through interaction with histones and mitigates histone acetylation and RNA polymerase II progression within transcribed regions contributing to the regulation of transcription (PubMed:21041482). Also functions as a deacetylase for non-histone targets, such as NR1D2, RELA, SP1, SP3, STAT3 and TSHZ3 (PubMed:12837748, PubMed:16285960, PubMed:16478997, PubMed:17996965, PubMed:19343227). Deacetylates SP proteins, SP1 and SP3, and regulates their function (PubMed:12837748, PubMed:16478997). Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons (PubMed:19081374). Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation (PubMed:19081374). Deacetylates TSHZ3 and regulates its transcriptional repressor activity (PubMed:19343227). Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B (PubMed:17000776). Deacetylates NR1D2 and abrogates the effect of KAT5-mediated relieving of NR1D2 transcription repression activity (PubMed:17996965). Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development (By similarity). Involved in CIART-mediated transcriptional repression of the circadian transcriptional activator: CLOCK-BMAL1 heterodimer (By similarity). Required for the transcriptional repression of circadian target genes, such as PER1, mediated by the large PER complex or CRY1 through histone deacetylation (By similarity). In addition to protein deacetylase activity, also has protein-lysine deacylase activity: acts as a protein decrotonylase and delactylase by mediating decrotonylation ((2E)-butenoyl) and delactylation (lactoyl) of histones, respectively (PubMed:28497810, PubMed:35044827).

Targets

HDAC8, HDAC3, HDAC2

Alternative names

Recommended products

Knockout Tested Rabbit Recombinant Multiclonal HDAC1 antibody. Suitable for Flow Cyt (Intra), IHC-P, IP, ICC/IF, WB and reacts with Human, Mouse, Rat samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Multiclonal

Immunogens
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

RM1172

Purification technique

Affinity purification Protein A

Specificity

Unsuitable for mouse and rat IP application.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This product is a recombinant multiclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Histone deacetylases 1 2 3 and 8 (HDAC1 HDAC2 HDAC3 HDAC8) are critical enzymes involved in chromatin remodeling and gene expression regulation. They operate by removing acetyl groups from lysine residues on histones leading to chromatin condensation and transcriptional repression. HDAC1 has a molecular weight of approximately 61 kDa HDAC2 is around 55 kDa HDAC3 approximately 48 kDa and HDAC8 about 41 kDa. These proteins are expressed in various tissues with high levels found in the brain liver and heart indicating their importance in multiple physiological processes.

Biological function summary

HDAC1 HDAC2 HDAC3 and HDAC8 play roles in transcriptional repression and cell cycle progression. They often exist as part of larger multiprotein complexes like the Sin3 NCoR and CoREST complexes which enhance their deacetylase activity. These enzymes participate in the regulation of genes involved in cell differentiation proliferation and apoptosis. Their activity is important for maintaining the balance of gene expression required for normal cellular function and development.

Pathways

HDAC1 HDAC2 HDAC3 and HDAC8 integrate into the regulation of critical signaling cascades such as the Notch and Wnt pathways. In the Notch pathway they interact with transcriptional repressors to adjust gene expression patterns during cell differentiation. Within these pathways HDAC proteins interact with other key proteins like JAG1 in the Notch pathway and β-catenin in the Wnt pathway modulating the transcriptional activity based on cellular contexts and stimuli.

Associated diseases and disorders

Dysfunction in HDAC1 HDAC2 HDAC3 and HDAC8 links to conditions such as cancer and neurodegenerative diseases. In cancer these HDACs can promote oncogenic processes by silencing tumor suppressor genes making them targets for cancer therapy. In neurodegenerative diseases like Alzheimer's alterations in their activity may disrupt normal neuronal function and gene expression patterns. The involvement with proteins such as p53 in cancer and tau proteins in neurodegenerative disorders highlights the critical role these HDACs play in disease mechanisms.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

13 product images

  • Western blot - Anti-HDAC1 + HDAC2 + HDAC3 + HDAC8 antibody [RM1172] (ab319059), expandable thumbnail

    Western blot - Anti-HDAC1 + HDAC2 + HDAC3 + HDAC8 antibody [RM1172] (ab319059)

    In Western blot, ab319059 was shown to bind specifically to HDAC1, HDAC2, HDAC3, HDAC8. Target of interest was observed at 55kDa in wild-type HAP1 cell lysates (lane 1) with no signal observed at this size in HDAC1 knockout cell line (lane 2).Target of interest was observed at 55kDa in wild-type 293T cell lysates (lane 3) with no signal observed at this size in HDAC2 knockout cell line (lane 4), (lane 4, knockout cell line Human HDAC2 knockout HEK-293T cell line ab266590 / knockout cell lysate Human HDAC2 knockout HEK-293T cell lysate ab256939).Target of interest was observed at 42kDa in wild-type HAP1 cell lysates (lane 5) with no signal observed at this size in HDAC8 knockout cell line (lane 6).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-HDAC1 + HDAC2 + HDAC3 + HDAC8 antibody [RM1172] (ab319059) at 1/1000 dilution

    Lane 1: Wild-type HAP1(human chronic myelogenous leukemia near-haploid cell ) whole cell lysate at 20 µg

    Lane 2: HDAC1 knockout HAP1whole cell lysate at 20 µg

    Lane 3: Wild-type 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Lane 4: HDAC2 knockout 293T whole cell lysate at 20 µg

    Lane 5: Wild-type HAP1 whole cell lysate at 20 µg

    Lane 6: HDAC8 knockout HAP1 whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Performed under reducing conditions.

    Observed band size: 42 kDa, 49 kDa, 55 kDa, 36 kDa

    Exposure time: 1s

  • Flow Cytometry (Intracellular) - Anti-HDAC1 + HDAC2 + HDAC3 + HDAC8 antibody [RM1172] (ab319059), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-HDAC1 + HDAC2 + HDAC3 + HDAC8 antibody [RM1172] (ab319059)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized C6 (rat glial tumor glial cell) cells labelling HDAC1 + HDAC2 + HDAC3 + HDAC8 with ab319059 at 1/500 dilution (0.1ug) / Magenta compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

  • Flow Cytometry (Intracellular) - Anti-HDAC1 + HDAC2 + HDAC3 + HDAC8 antibody [RM1172] (ab319059), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-HDAC1 + HDAC2 + HDAC3 + HDAC8 antibody [RM1172] (ab319059)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling HDAC1 + HDAC2 + HDAC3 + HDAC8 with ab319059 at 1/500 dilution (0.1ug) / Magenta compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

  • Flow Cytometry (Intracellular) - Anti-HDAC1 + HDAC2 + HDAC3 + HDAC8 antibody [RM1172] (ab319059), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-HDAC1 + HDAC2 + HDAC3 + HDAC8 antibody [RM1172] (ab319059)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling HDAC1 + HDAC2 + HDAC3 + HDAC8 with ab319059 at 1/500 dilution (0.1ug) / Magenta compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

  • Immunocytochemistry/ Immunofluorescence - Anti-HDAC1 + HDAC2 + HDAC3 + HDAC8 antibody [RM1172] (ab319059), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-HDAC1 + HDAC2 + HDAC3 + HDAC8 antibody [RM1172] (ab319059)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized C6 (rat glial tumor glial cell) cells labelling HDAC1 + HDAC2 + HDAC3 + HDAC8 with ab319059 at 1/1000 (0.502 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

    Confocal image showing nuclear staining in C6 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-HDAC1 + HDAC2 + HDAC3 + HDAC8 antibody [RM1172] (ab319059), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-HDAC1 + HDAC2 + HDAC3 + HDAC8 antibody [RM1172] (ab319059)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling HDAC1 + HDAC2 + HDAC3 + HDAC8 with ab319059 at 1/1000 (0.502 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

    Confocal image showing nuclear staining in RAW 264.7 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-HDAC1 + HDAC2 + HDAC3 + HDAC8 antibody [RM1172] (ab319059), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-HDAC1 + HDAC2 + HDAC3 + HDAC8 antibody [RM1172] (ab319059)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling HDAC1 + HDAC2 + HDAC3 + HDAC8 with ab319059 at 1/1000 (0.502 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

    Confocal image showing nuclear staining in Hela cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Western blot - Anti-HDAC1 + HDAC2 + HDAC3 + HDAC8 antibody [RM1172] (ab319059), expandable thumbnail

    Western blot - Anti-HDAC1 + HDAC2 + HDAC3 + HDAC8 antibody [RM1172] (ab319059)

    Exposure time: Lanes 1-4: 10 seconds Lanes 5-9: 48 seconds

    All lanes: Western blot - Anti-HDAC1 + HDAC2 + HDAC3 + HDAC8 antibody [RM1172] (ab319059) at 1/1000 dilution

    Lane 1: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate at 20 µg

    Lane 3: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 4: K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

    Lane 5: HT-22 (mouse hippocampal neuronal cell) whole cell lysate at 20 µg

    Lane 6: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

    Lane 7: C6 (rat glial tumor glial cell) whole cell lysate at 20 µg

    Lane 8: RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

    Lane 9: L6 (rat skeletal muscle myoblast) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 42 kDa, 49 kDa, 55 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 + HDAC2 + HDAC3 + HDAC8 antibody [RM1172] (ab319059), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 + HDAC2 + HDAC3 + HDAC8 antibody [RM1172] (ab319059)

    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling HDAC1 + HDAC2 + HDAC3 + HDAC8 with ab319059 at 1/5000 (0.1 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse colon. The section was incubated with ab319059 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Western blot - Anti-HDAC1 + HDAC2 + HDAC3 + HDAC8 antibody [RM1172] (ab319059), expandable thumbnail

    Western blot - Anti-HDAC1 + HDAC2 + HDAC3 + HDAC8 antibody [RM1172] (ab319059)

    The identity of the lower MW band at approximately 35 kDa is unknown.

    Lanes 1-2 are applied with Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 and lanes 3-6 are applied with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000.

    All lanes: Western blot - Anti-HDAC1 + HDAC2 + HDAC3 + HDAC8 antibody [RM1172] (ab319059) at 1/1000 dilution

    Lane 1: Human liver tissue lysate at 20 µg

    Lane 2: Human spleen tissue lysate at 20 µg

    Lane 3: Mouse brain tissue lysate at 20 µg

    Lane 4: Mouse spleen tissue lysate at 20 µg

    Lane 5: Rat heart tissue lysate at 20 µg

    Lane 6: Rat spleen tissue lysate at 20 µg

    Secondary

    Lanes 1 - 2: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Lanes 3 - 6: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 42 kDa, 49 kDa, 55 kDa

    Exposure time: 92s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 + HDAC2 + HDAC3 + HDAC8 antibody [RM1172] (ab319059), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 + HDAC2 + HDAC3 + HDAC8 antibody [RM1172] (ab319059)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling HDAC1 + HDAC2 + HDAC3 + HDAC8 with ab319059 at 1/5000 (0.1 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human colon. The section was incubated with ab319059 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 + HDAC2 + HDAC3 + HDAC8 antibody [RM1172] (ab319059), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 + HDAC2 + HDAC3 + HDAC8 antibody [RM1172] (ab319059)

    Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling HDAC1 + HDAC2 + HDAC3 + HDAC8 with ab319059 at 1/5000 (0.1 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on rat colon. The section was incubated with ab319059 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunoprecipitation - Anti-HDAC1 + HDAC2 + HDAC3 + HDAC8 antibody [RM1172] (ab319059), expandable thumbnail

    Immunoprecipitation - Anti-HDAC1 + HDAC2 + HDAC3 + HDAC8 antibody [RM1172] (ab319059)

    HDAC1 + HDAC2 + HDAC3 + HDAC8 was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with ab319059 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab319059 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

    Lane 2: ab319059 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab319059 in HeLa whole cell lysate

    All lanes: Immunoprecipitation - Anti-HDAC1 + HDAC2 + HDAC3 + HDAC8 antibody [RM1172] (ab319059) at 1/30 dilution

    All lanes: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 15s

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Product protocols

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