Anti-HDAC10 antibody [EPR3576]

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-HDAC10 antibody [EPR3576] (AB108934)

Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling HDAC10 with Purified ab108934 at 1/200 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488 ,ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-HDAC10 antibody [EPR3576] (AB108934)

Immunocytochemistry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling HDAC10 with Purified ab108934 at 1 : 200 dilution (8.75 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488,ab150077) was used as the secondary antibody at 1 : 1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• WB

Unknown

Western blot - Anti-HDAC10 antibody [EPR3576] (AB108934)

The unboiled lysate is recommended in order to prevent the aggregation of proteins.

All lanes:

Western blot - Anti-HDAC10 antibody [EPR3576] (ab108934) at 1/5000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate boiled at 20 µg

Lane 2:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate unboiled at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 71 kDa

false

• IP

Lab

Immunoprecipitation - Anti-HDAC10 antibody [EPR3576] (AB108934)

Purified ab108934 at 1/100 dilution (2μg) immunoprecipitating HDAC10 in HeLa whole cell lysate.
Lane 1 (input) : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10μg
Lane 2 (+) : ab108934 + HeLa whole cell lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab108934 in HeLa whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 75 kDa

All lanes:

Immunoprecipitation - Anti-HDAC10 antibody [EPR3576] (ab108934)

Predicted band size: 71 kDa

false

• WB

Unknown

Western blot - Anti-HDAC10 antibody [EPR3576] (AB108934)

All lanes:

Western blot - Anti-HDAC10 antibody [EPR3576] (ab108934) at 1/1000 dilution

Lane 1:

293T (Human embryonic kidney epithelial cell) transfected with the empty vector containing the myc-His-tag® at 20 µg

Lane 2:

293T (Human embryonic kidney epithelial cell) transfected with human HDAC10 expression vector containing the myc-His-tag® at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 71 kDa

false

• WB

Unknown

Western blot - Anti-HDAC10 antibody [EPR3576] (AB108934)

All lanes:

Western blot - Anti-HDAC10 antibody [EPR3576] (ab108934) at 1/1000 dilution

All lanes:

N-terminal GST-tagged human HDAC10 recombinant protein fragment (aa 1 to 482)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 71 kDa

false