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AB315883

Anti-HDAC11/HD11 antibody [EPR26890-83]

  • 20ul selling size
  • Recombinant
  • Advanced Validation
  • RabMAb
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Rabbit Recombinant Monoclonal HDAC11/HD11 antibody. Suitable for WB, ChIP-seq, IP and reacts with Mouse, Rat samples.

View Alternative Names

Histone deacetylase 11, HD11, Hdac11

6 Images
Immunoprecipitation - Anti-HDAC11/HD11 antibody [EPR26890-83] (AB315883)
  • IP

Supplier Data

Immunoprecipitation - Anti-HDAC11/HD11 antibody [EPR26890-83] (AB315883)

HDAC11/HD11 was immunoprecipitated from 0.35 mg MEF (mouse embryo fibroblast) whole cell lysate with ab315883 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab315883 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : MEF (mouse embryo fibroblast) whole cell lysate
Lane 2 : ab315883 IP in MEF (mouse embryo fibroblast) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab315883 in MEF whole cell lysate

All lanes:

Immunoprecipitation - Anti-HDAC11/HD11 antibody [EPR26890-83] (ab315883) at 1/30 dilution

All lanes:

MEF (mouse embryo fibroblast) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 180s

Immunoprecipitation - Anti-HDAC11/HD11 antibody [EPR26890-83] (AB315883)
  • IP

Supplier Data

Immunoprecipitation - Anti-HDAC11/HD11 antibody [EPR26890-83] (AB315883)

HDAC11/HD11 was immunoprecipitated from 0.35 mg Mouse brain tissue lysate with ab315883 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab315883 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Mouse brain tissue lysate
Lane 2 : ab315883 IP in Mouse brain tissue lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab315883 in mouse brain tissue lysate

All lanes:

Immunoprecipitation - Anti-HDAC11/HD11 antibody [EPR26890-83] (ab315883) at 1/30 dilution

All lanes:

Mouse brain tissue lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 180s

ChIP-sequencing - Anti-HDAC11/HD11 antibody [EPR26890-83] (AB315883)
  • ChIP-seq

Supplier Data

ChIP-sequencing - Anti-HDAC11/HD11 antibody [EPR26890-83] (AB315883)

ChIP-sequencing - Anti-HDAC11/HD11 antibody [EPR26890-83] (ab315883)

Chromatin was prepared from MEF (mouse embryo fibroblast) cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10^7 cells and 8 µg of ab315883 [EPR26890-83]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.

ChIP-sequencing - Anti-HDAC11/HD11 antibody [EPR26890-83] (AB315883)
  • ChIP-seq

Supplier Data

ChIP-sequencing - Anti-HDAC11/HD11 antibody [EPR26890-83] (AB315883)

ChIP-sequencing - Anti-HDAC11/HD11 antibody [EPR26890-83] (ab315883)

Chromatin was prepared from MEF (mouse embryo fibroblast) cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10^7 cells and 8 µg of ab315883 [EPR26890-83]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.

ChIP-sequencing - Anti-HDAC11/HD11 antibody [EPR26890-83] (AB315883)
  • ChIP-seq

Supplier Data

ChIP-sequencing - Anti-HDAC11/HD11 antibody [EPR26890-83] (AB315883)

ChIP-sequencing - Anti-HDAC11/HD11 antibody [EPR26890-83] (ab315883)

Chromatin was prepared from MEF (mouse embryo fibroblast) cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10^7 cells and 8 µg of ab315883 [EPR26890-83]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.

Western blot - Anti-HDAC11/HD11 antibody [EPR26890-83] (AB315883)
  • WB

Supplier Data

Western blot - Anti-HDAC11/HD11 antibody [EPR26890-83] (AB315883)

Low expression : liver, spleen (PMID : 11948178)

The identity of the lower MW band below 39kDa is unknow.

The identity of the higher MW band at approximately 200kDa in lane 1 and lane 4 is unknown.

Lysate was freshly made and used for Western blotting immediately to minimize protein degradation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-HDAC11/HD11 antibody [EPR26890-83] (ab315883) at 1/1000 dilution

Lane 1:

Rat brain tissue lysate at 20 µg

Lane 2:

Rat liver tissue lysate at 20 µg

Lane 3:

Rat spleen tissue lysate at 20 µg

Lane 4:

Mouse brain tissue lysate at 20 µg

Lane 5:

Mouse liver tissue lysate at 20 µg

Lane 6:

Mouse spleen tissue lysate at 20 µg

Lane 7:

MEF (mouse embryo fibroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 39 kDa,36 kDa

false

Exposure time: 180s

  • Carrier free

    Anti-HDAC11/HD11 antibody [EPR26890-83] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26890-83

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat

Applications

WB, ChIP-seq, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Proteins & Peptides

AB101113

Recombinant Human HDAC11/HD11 protein

Functional Studies - Recombinant Human HDAC11/HD11 protein (AB101113)

  • 0
  • 0
Primary Antibodies

AB76541

Anti-CDX2 antibody [EPR2764Y]

RabMAb|Recombinant|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [EPR2764Y] (AB76541)

  • 4
  • 17
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ChIPseq" : {"fullname" : "ChIP-sequencing", "shortname":"ChIP-seq"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ChIPseq-species-checked": "notRecommended", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p>HDAC11 is a protein featuring high lability, with a half-life of approximately 4 hours. Consequently, it is easy to observe multiple bands of this protein in western blot analysis (PMID: 31746627).</p>", "ChIPseq-species-checked": "testedAndGuaranteed", "ChIPseq-species-dilution-info": "8 µg for 10^7 Cells", "ChIPseq-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p>HDAC11 is a protein featuring high lability, with a half-life of approximately 4 hours. Consequently, it is easy to observe multiple bands of this protein in western blot analysis (PMID: 31746627).</p>", "ChIPseq-species-checked": "guaranteed", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HDAC11 also known as HD11 is a unique member of the histone deacetylase family. HDAC11 is an enzyme involved in removing acetyl groups from lysine residues on histone tails affecting chromatin structure and gene expression. It has a molecular mass of approximately 39 kDa. You can find HDAC11 expressed in various tissues but it shows higher expression levels in brain heart skeletal muscles and kidneys indicating its significance in these organs.
Biological function summary

HDAC11 participates in the regulation of gene expression by modulating histone acetylation levels. It often functions as a part of large protein complexes that include other histone deacetylases and transcriptional co-repressors. By altering acetylation status HDAC11 can influence epigenetic regulation and thereby affect numerous cellular processes such as cell cycle progression differentiation and apoptosis. Its activity contributes to maintaining cellular homeostasis.

Pathways

HDAC11 plays a role in key signaling pathways such as the DNA damage response and inflammatory pathways. In the DNA damage response pathway HDAC11 interacts with proteins like HDAC1 and HDAC2 which are essential for DNA repair processes. Additionally in inflammatory pathways HDAC11 regulates cytokine expression and immune cell function highlighting its role in immune response modulation.

Researchers have linked HDAC11 to cancer and autoimmune diseases. Its abnormal expression or activity has associations with various cancer types including brain and kidney cancers where it potentially influences tumor progression through interaction with proteins like p53. In autoimmune diseases HDAC11 affects immune cell regulation and cytokine production interacting with immune-related proteins which may contribute to disease development and progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity).
See full target information Hdac11
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com