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Rabbit Recombinant Monoclonal HDAC11/HD11 antibody. Suitable for WB, ChIP-seq, IP and reacts with Mouse, Rat samples.

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Images

Western blot - Anti-HDAC11/HD11 antibody [EPR26890-83] (AB315883), expandable thumbnail
  • ChIP-sequencing - Anti-HDAC11/HD11 antibody [EPR26890-83] (AB315883), expandable thumbnail
  • ChIP-sequencing - Anti-HDAC11/HD11 antibody [EPR26890-83] (AB315883), expandable thumbnail
  • ChIP-sequencing - Anti-HDAC11/HD11 antibody [EPR26890-83] (AB315883), expandable thumbnail
  • Immunoprecipitation - Anti-HDAC11/HD11 antibody [EPR26890-83] (AB315883), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBChIP-seqIPIHC-PFlow Cyt (Intra)ICC/IF
Human
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Tested
Tested
Not recommended
Not recommended
Not recommended
Rat
Tested
Expected
Expected
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

HDAC11 is a protein featuring high lability, with a half-life of approximately 4 hours. Consequently, it is easy to observe multiple bands of this protein in western blot analysis (PMID: 31746627).

Species
Rat
Dilution info
1/1000
Notes

HDAC11 is a protein featuring high lability, with a half-life of approximately 4 hours. Consequently, it is easy to observe multiple bands of this protein in western blot analysis (PMID: 31746627).

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
8 µg for 107 Cells
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/30
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity).

Alternative names

Histone deacetylase 11, HD11, Hdac11

Recommended products

Rabbit Recombinant Monoclonal HDAC11/HD11 antibody. Suitable for WB, ChIP-seq, IP and reacts with Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR26890-83
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

HDAC11 also known as HD11 is a unique member of the histone deacetylase family. HDAC11 is an enzyme involved in removing acetyl groups from lysine residues on histone tails affecting chromatin structure and gene expression. It has a molecular mass of approximately 39 kDa. You can find HDAC11 expressed in various tissues but it shows higher expression levels in brain heart skeletal muscles and kidneys indicating its significance in these organs.

Biological function summary

HDAC11 participates in the regulation of gene expression by modulating histone acetylation levels. It often functions as a part of large protein complexes that include other histone deacetylases and transcriptional co-repressors. By altering acetylation status HDAC11 can influence epigenetic regulation and thereby affect numerous cellular processes such as cell cycle progression differentiation and apoptosis. Its activity contributes to maintaining cellular homeostasis.

Pathways

HDAC11 plays a role in key signaling pathways such as the DNA damage response and inflammatory pathways. In the DNA damage response pathway HDAC11 interacts with proteins like HDAC1 and HDAC2 which are essential for DNA repair processes. Additionally in inflammatory pathways HDAC11 regulates cytokine expression and immune cell function highlighting its role in immune response modulation.

Associated diseases and disorders

Researchers have linked HDAC11 to cancer and autoimmune diseases. Its abnormal expression or activity has associations with various cancer types including brain and kidney cancers where it potentially influences tumor progression through interaction with proteins like p53. In autoimmune diseases HDAC11 affects immune cell regulation and cytokine production interacting with immune-related proteins which may contribute to disease development and progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-HDAC11/HD11 antibody [EPR26890-83] (ab315883), expandable thumbnail

    Western blot - Anti-HDAC11/HD11 antibody [EPR26890-83] (ab315883)

    Low expression: liver, spleen (PMID: 11948178)

    The identity of the lower MW band below 39kDa is unknow.

    The identity of the higher MW band at approximately 200kDa in lane 1 and lane 4 is unknown.

    Lysate was freshly made and used for Western blotting immediately to minimize protein degradation.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-HDAC11/HD11 antibody [EPR26890-83] (ab315883) at 1/1000 dilution

    Lane 1: Rat brain tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 2: Rat liver tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 3: Rat spleen tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 4: Mouse brain tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 5: Mouse liver tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 6: Mouse spleen tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 7: MEF (mouse embryo fibroblast) whole cell lysate at 20 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 39 kDa, 36 kDa

    Exposure time: 180s

  • ChIP-sequencing - Anti-HDAC11/HD11 antibody [EPR26890-83] (ab315883), expandable thumbnail

    ChIP-sequencing - Anti-HDAC11/HD11 antibody [EPR26890-83] (ab315883)

    ChIP-sequencing - Anti-HDAC11/HD11 antibody [EPR26890-83] (ab315883)

    Chromatin was prepared from MEF (mouse embryo fibroblast) cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10^7 cells and 8 µg of ab315883 [EPR26890-83]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.

  • ChIP-sequencing - Anti-HDAC11/HD11 antibody [EPR26890-83] (ab315883), expandable thumbnail

    ChIP-sequencing - Anti-HDAC11/HD11 antibody [EPR26890-83] (ab315883)

    ChIP-sequencing - Anti-HDAC11/HD11 antibody [EPR26890-83] (ab315883)

    Chromatin was prepared from MEF (mouse embryo fibroblast) cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10^7 cells and 8 µg of ab315883 [EPR26890-83]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.

  • ChIP-sequencing - Anti-HDAC11/HD11 antibody [EPR26890-83] (ab315883), expandable thumbnail

    ChIP-sequencing - Anti-HDAC11/HD11 antibody [EPR26890-83] (ab315883)

    ChIP-sequencing - Anti-HDAC11/HD11 antibody [EPR26890-83] (ab315883)

    Chromatin was prepared from MEF (mouse embryo fibroblast) cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10^7 cells and 8 µg of ab315883 [EPR26890-83]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.

  • Immunoprecipitation - Anti-HDAC11/HD11 antibody [EPR26890-83] (ab315883), expandable thumbnail

    Immunoprecipitation - Anti-HDAC11/HD11 antibody [EPR26890-83] (ab315883)

    HDAC11/HD11 was immunoprecipitated from 0.35 mg MEF (mouse embryo fibroblast) whole cell lysate with ab315883 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab315883 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: MEF (mouse embryo fibroblast) whole cell lysate

    Lane 2: ab315883 IP in MEF (mouse embryo fibroblast) whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab315883 in MEF whole cell lysate

    All lanes: Immunoprecipitation - Anti-HDAC11/HD11 antibody [EPR26890-83] (ab315883) at 1/30 dilution

    All lanes: MEF (mouse embryo fibroblast) whole cell lysate with 5% NFDM/TBST

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 180s

  • Immunoprecipitation - Anti-HDAC11/HD11 antibody [EPR26890-83] (ab315883), expandable thumbnail

    Immunoprecipitation - Anti-HDAC11/HD11 antibody [EPR26890-83] (ab315883)

    HDAC11/HD11 was immunoprecipitated from 0.35 mg Mouse brain tissue lysate with ab315883 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab315883 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse brain tissue lysate

    Lane 2: ab315883 IP in Mouse brain tissue lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab315883 in mouse brain tissue lysate

    All lanes: Immunoprecipitation - Anti-HDAC11/HD11 antibody [EPR26890-83] (ab315883) at 1/30 dilution

    All lanes: Mouse brain tissue lysate with 5% NFDM/TBST

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 180s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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