Rabbit Recombinant Monoclonal HDAC11/HD11 antibody. Carrier free. Suitable for WB, ChIP-seq, IP and reacts with Mouse, Rat samples.
Images
![Western blot - Anti-HDAC11/HD11 antibody [EPR26890-83] - BSA and Azide free (AB315884), expandable thumbnail](https://content.abcam.com/products/images/hdac11-hd11-antibody-epr26890-83-bsa-and-azide-free-ab315884--western-blot-img427818.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-HDAC11/HD11 antibody [EPR26890-83] - BSA and Azide free (AB315884)")
![ChIP-sequencing - Anti-HDAC11/HD11 antibody [EPR26890-83] - BSA and Azide free (AB315884), expandable thumbnail](https://content.abcam.com/products/images/hdac11-hd11-antibody-epr26890-83-bsa-and-azide-free-ab315884--chip-sequencing-img427871.jpg/_jcr_content/renditions/webp-475.webp "ChIP-sequencing - Anti-HDAC11/HD11 antibody [EPR26890-83] - BSA and Azide free (AB315884)")
![ChIP-sequencing - Anti-HDAC11/HD11 antibody [EPR26890-83] - BSA and Azide free (AB315884), expandable thumbnail](https://content.abcam.com/products/images/hdac11-hd11-antibody-epr26890-83-bsa-and-azide-free-ab315884--chip-sequencing-img427870.jpg/_jcr_content/renditions/webp-475.webp "ChIP-sequencing - Anti-HDAC11/HD11 antibody [EPR26890-83] - BSA and Azide free (AB315884)")
![ChIP-sequencing - Anti-HDAC11/HD11 antibody [EPR26890-83] - BSA and Azide free (AB315884), expandable thumbnail](https://content.abcam.com/products/images/hdac11-hd11-antibody-epr26890-83-bsa-and-azide-free-ab315884--chip-sequencing-img427869.jpg/_jcr_content/renditions/webp-475.webp "ChIP-sequencing - Anti-HDAC11/HD11 antibody [EPR26890-83] - BSA and Azide free (AB315884)")
![Immunoprecipitation - Anti-HDAC11/HD11 antibody [EPR26890-83] - BSA and Azide free (AB315884), expandable thumbnail](https://content.abcam.com/products/images/hdac11-hd11-antibody-epr26890-83-bsa-and-azide-free-ab315884--immunoprecipitation-img427828.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-HDAC11/HD11 antibody [EPR26890-83] - BSA and Azide free (AB315884)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ChIP-seq | IP | IHC-P | Flow Cyt (Intra) | ICC/IF | |
|---|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Tested | Tested | Not recommended | Not recommended | Not recommended |
| Rat | Tested | Expected | Expected | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes HDAC11 is a protein featuring high lability, with a half-life of approximately 4 hours. Consequently, it is easy to observe multiple bands of this protein in western blot analysis (PMID: 31746627). |
Species Rat | Dilution info - | Notes HDAC11 is a protein featuring high lability, with a half-life of approximately 4 hours. Consequently, it is easy to observe multiple bands of this protein in western blot analysis (PMID: 31746627). |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ChIP-seq
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity).
Alternative names
Histone deacetylase 11, HD11, Hdac11
Recommended products
Rabbit Recombinant Monoclonal HDAC11/HD11 antibody. Carrier free. Suitable for WB, ChIP-seq, IP and reacts with Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR26890-83
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab315884 is the carrier-free version of Anti-HDAC11/HD11 antibody [EPR26890-83] ab315883.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
HDAC11 also known as HD11 is a unique member of the histone deacetylase family. HDAC11 is an enzyme involved in removing acetyl groups from lysine residues on histone tails affecting chromatin structure and gene expression. It has a molecular mass of approximately 39 kDa. You can find HDAC11 expressed in various tissues but it shows higher expression levels in brain heart skeletal muscles and kidneys indicating its significance in these organs.
- Biological function summary
HDAC11 participates in the regulation of gene expression by modulating histone acetylation levels. It often functions as a part of large protein complexes that include other histone deacetylases and transcriptional co-repressors. By altering acetylation status HDAC11 can influence epigenetic regulation and thereby affect numerous cellular processes such as cell cycle progression differentiation and apoptosis. Its activity contributes to maintaining cellular homeostasis.
- Pathways
HDAC11 plays a role in key signaling pathways such as the DNA damage response and inflammatory pathways. In the DNA damage response pathway HDAC11 interacts with proteins like HDAC1 and HDAC2 which are essential for DNA repair processes. Additionally in inflammatory pathways HDAC11 regulates cytokine expression and immune cell function highlighting its role in immune response modulation.
- Associated diseases and disorders
Researchers have linked HDAC11 to cancer and autoimmune diseases. Its abnormal expression or activity has associations with various cancer types including brain and kidney cancers where it potentially influences tumor progression through interaction with proteins like p53. In autoimmune diseases HDAC11 affects immune cell regulation and cytokine production interacting with immune-related proteins which may contribute to disease development and progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-HDAC11/HD11 antibody [EPR26890-83] - BSA and Azide free (ab315884), expandable thumbnail](https://content.abcam.com/products/images/hdac11-hd11-antibody-epr26890-83-bsa-and-azide-free-ab315884--western-blot-img427818.jpg "Western blot - Anti-HDAC11/HD11 antibody [EPR26890-83] - BSA and Azide free (ab315884)")
Western blot - Anti-HDAC11/HD11 antibody [EPR26890-83] - BSA and Azide free (ab315884)
This data was developed using Anti-HDAC11/HD11 antibody [EPR26890-83] ab315883, the same antibody clone in a different buffer formulation.
Low expression: liver, spleen (PMID: 11948178)
The identity of the lower MW band below 39kDa is unknow.
The identity of the higher MW band at approximately 200kDa in lane 1 and lane 4 is unknown.
Lysate was freshly made and used for Western blotting immediately to minimize protein degradation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-HDAC11/HD11 antibody [EPR26890-83] (Anti-HDAC11/HD11 antibody [EPR26890-83] ab315883) at 1/1000 dilution
Lane 1: Rat brain tissue lysate at 20 µg with 5% NFDM/TBST
Lane 2: Rat liver tissue lysate at 20 µg with 5% NFDM/TBST
Lane 3: Rat spleen tissue lysate at 20 µg with 5% NFDM/TBST
Lane 4: Mouse brain tissue lysate at 20 µg with 5% NFDM/TBST
Lane 5: Mouse liver tissue lysate at 20 µg with 5% NFDM/TBST
Lane 6: Mouse spleen tissue lysate at 20 µg with 5% NFDM/TBST
Lane 7: MEF (mouse embryo fibroblast) whole cell lysate at 20 µg with 5% NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 39 kDa, 36 kDa
Exposure time: 180s
![ChIP-sequencing - Anti-HDAC11/HD11 antibody [EPR26890-83] - BSA and Azide free (ab315884), expandable thumbnail](https://content.abcam.com/products/images/hdac11-hd11-antibody-epr26890-83-bsa-and-azide-free-ab315884--chip-sequencing-img427871.jpg "ChIP-sequencing - Anti-HDAC11/HD11 antibody [EPR26890-83] - BSA and Azide free (ab315884)")
ChIP-sequencing - Anti-HDAC11/HD11 antibody [EPR26890-83] - BSA and Azide free (ab315884)
This data was developed using Anti-HDAC11/HD11 antibody [EPR26890-83] ab315883, the same antibody clone in a different buffer formulation.
ChIP-sequencing - Anti-HDAC11/HD11 antibody [EPR26890-83] (Anti-HDAC11/HD11 antibody [EPR26890-83] ab315883)
Chromatin was prepared from MEF (mouse embryo fibroblast) cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10^7 cells and 8 µg of Anti-HDAC11/HD11 antibody [EPR26890-83] ab315883 [EPR26890-83]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.
![ChIP-sequencing - Anti-HDAC11/HD11 antibody [EPR26890-83] - BSA and Azide free (ab315884), expandable thumbnail](https://content.abcam.com/products/images/hdac11-hd11-antibody-epr26890-83-bsa-and-azide-free-ab315884--chip-sequencing-img427870.jpg "ChIP-sequencing - Anti-HDAC11/HD11 antibody [EPR26890-83] - BSA and Azide free (ab315884)")
ChIP-sequencing - Anti-HDAC11/HD11 antibody [EPR26890-83] - BSA and Azide free (ab315884)
This data was developed using Anti-HDAC11/HD11 antibody [EPR26890-83] ab315883, the same antibody clone in a different buffer formulation.
ChIP-sequencing - Anti-HDAC11/HD11 antibody [EPR26890-83] (Anti-HDAC11/HD11 antibody [EPR26890-83] ab315883)
Chromatin was prepared from MEF (mouse embryo fibroblast) cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10^7 cells and 8 µg of Anti-HDAC11/HD11 antibody [EPR26890-83] ab315883 [EPR26890-83]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.
![ChIP-sequencing - Anti-HDAC11/HD11 antibody [EPR26890-83] - BSA and Azide free (ab315884), expandable thumbnail](https://content.abcam.com/products/images/hdac11-hd11-antibody-epr26890-83-bsa-and-azide-free-ab315884--chip-sequencing-img427869.jpg "ChIP-sequencing - Anti-HDAC11/HD11 antibody [EPR26890-83] - BSA and Azide free (ab315884)")
ChIP-sequencing - Anti-HDAC11/HD11 antibody [EPR26890-83] - BSA and Azide free (ab315884)
This data was developed using Anti-HDAC11/HD11 antibody [EPR26890-83] ab315883, the same antibody clone in a different buffer formulation.
ChIP-sequencing - Anti-HDAC11/HD11 antibody [EPR26890-83] (Anti-HDAC11/HD11 antibody [EPR26890-83] ab315883)
Chromatin was prepared from MEF (mouse embryo fibroblast) cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10^7 cells and 8 µg of Anti-HDAC11/HD11 antibody [EPR26890-83] ab315883 [EPR26890-83]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.
![Immunoprecipitation - Anti-HDAC11/HD11 antibody [EPR26890-83] - BSA and Azide free (ab315884), expandable thumbnail](https://content.abcam.com/products/images/hdac11-hd11-antibody-epr26890-83-bsa-and-azide-free-ab315884--immunoprecipitation-img427828.jpg "Immunoprecipitation - Anti-HDAC11/HD11 antibody [EPR26890-83] - BSA and Azide free (ab315884)")
Immunoprecipitation - Anti-HDAC11/HD11 antibody [EPR26890-83] - BSA and Azide free (ab315884)
This data was developed using Anti-HDAC11/HD11 antibody [EPR26890-83] ab315883, the same antibody clone in a different buffer formulation.
HDAC11/HD11 was immunoprecipitated from 0.35 mg MEF (mouse embryo fibroblast) whole cell lysate with Anti-HDAC11/HD11 antibody [EPR26890-83] ab315883 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-HDAC11/HD11 antibody [EPR26890-83] ab315883 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: MEF (mouse embryo fibroblast) whole cell lysate
Lane 2: Anti-HDAC11/HD11 antibody [EPR26890-83] ab315883 IP in MEF (mouse embryo fibroblast) whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-HDAC11/HD11 antibody [EPR26890-83] ab315883 in MEF whole cell lysateAll lanes: Immunoprecipitation - Anti-HDAC11/HD11 antibody [EPR26890-83] (Anti-HDAC11/HD11 antibody [EPR26890-83] ab315883) at 1/30 dilution
All lanes: MEF (mouse embryo fibroblast) whole cell lysate with 5% NFDM/TBST
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 180s
![Immunoprecipitation - Anti-HDAC11/HD11 antibody [EPR26890-83] - BSA and Azide free (ab315884), expandable thumbnail](https://content.abcam.com/products/images/hdac11-hd11-antibody-epr26890-83-bsa-and-azide-free-ab315884--immunoprecipitation-img427827.jpg "Immunoprecipitation - Anti-HDAC11/HD11 antibody [EPR26890-83] - BSA and Azide free (ab315884)")
Immunoprecipitation - Anti-HDAC11/HD11 antibody [EPR26890-83] - BSA and Azide free (ab315884)
This data was developed using Anti-HDAC11/HD11 antibody [EPR26890-83] ab315883, the same antibody clone in a different buffer formulation.
HDAC11/HD11 was immunoprecipitated from 0.35 mg Mouse brain tissue lysate with Anti-HDAC11/HD11 antibody [EPR26890-83] ab315883 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-HDAC11/HD11 antibody [EPR26890-83] ab315883 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Mouse brain tissue lysate
Lane 2: Anti-HDAC11/HD11 antibody [EPR26890-83] ab315883 IP in Mouse brain tissue lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-HDAC11/HD11 antibody [EPR26890-83] ab315883 in mouse brain tissue lysateAll lanes: Immunoprecipitation - Anti-HDAC11/HD11 antibody [EPR26890-83] (Anti-HDAC11/HD11 antibody [EPR26890-83] ab315883) at 1/30 dilution
All lanes: Mouse brain tissue lysate with 5% NFDM/TBST
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 180s
![Immunoprecipitation - Anti-HDAC11/HD11 antibody [EPR26890-83] - BSA and Azide free (ab315884), expandable thumbnail](https://content.abcam.com/products/images/hdac11-hd11-antibody-epr26890-83-bsa-and-azide-free-ab315884--immunoprecipitation-img427827.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-HDAC11/HD11 antibody [EPR26890-83] - BSA and Azide free (ab315884)")
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