Anti-HDAC2 antibody - C-terminal

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HDAC2 antibody - C-terminal (AB227149)

Immunofluorescence analysis of 4% paraformaldehyde fixed at RT for 15 mins HeLa cells labelling HDAC2 protein at nucleus using ab227149 at a 1/500 dilution (Green). The nuclear counterstain is DAPI (Blue).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HDAC2 antibody - C-terminal (AB227149)

Immunofluorescence analysis of 4% paraformaldehyde fixed at RT for 15 mins HeLa cells labelling HDAC2 protein at nucleus using ab227149 at a 1/500 dilution(Green). The nuclear counterstain is DAPI (Blue).

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC2 antibody - C-terminal (AB227149)

Paraffin embedded Cal27 (human) xenograft tissue stained for HDAC2 using ab227149 at 1/100 dilution in immunohistochemical analysis.

• IP

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Immunoprecipitation - Anti-HDAC2 antibody - C-terminal (AB227149)

HDAC2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate. Western blot was performed from the immunoprecipitate using ab227149 at 1/1000 dilution. Anti-rabbit IgG was used as a secondary reagent.

Lane 1 : 40 μg HeLa whole cell lysate.

Lane 2 : Control with 2 μg of preimmune rabbit IgG.

Lane 3 : Immunoprecipitation of HDAC2 protein by 2 μg of ab227149.

7.5% SDS-PAGE gel.

All lanes:

Immunoprecipitation - Anti-HDAC2 antibody - C-terminal (ab227149)

Predicted band size: 55 kDa

false

• ChIP

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ChIP - Anti-HDAC2 antibody - C-terminal (AB227149)

ab227149 immunoprecipitates HDAC2 protein-DNA complex in ChIP experiments.

ChIP Sample : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate/extract.
A : 5 μg preimmune rabbit IgG
B : 5 μg of ab227149
The precipitated DNA was detected by PCR with primer set targeting to GAPDH promoter.

• WB

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Western blot - Anti-HDAC2 antibody - C-terminal (AB227149)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-HDAC2 antibody - C-terminal (ab227149) at 1/1000 dilution

Lane 1:

293T Whole cell extract at 30 µg

Lane 2:

A431 Whole cell extract at 30 µg

Lane 3:

HeLa whole cell lysate at 30 µg

Lane 4:

HepG2 Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 55 kDa

false

• WB

Supplier Data

Western blot - Anti-HDAC2 antibody - C-terminal (AB227149)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-HDAC2 antibody - C-terminal (ab227149) at 1/1000 dilution

All lanes:

Rat2 (rat fibroblast cell line) whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 55 kDa

false

• WB

Supplier Data

Western blot - Anti-HDAC2 antibody - C-terminal (AB227149)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-HDAC2 antibody - C-terminal (ab227149) at 1/1000 dilution

All lanes:

C2C12 (mouse myoblast cell line) whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 55 kDa

false