Anti-HDAC2 antibody [Y461] - BSA and Azide free (ab213700)

Knockout Tested Rabbit Recombinant Monoclonal HDAC2 antibody. Carrier free. Suitable for ChIC/CUT&RUN-seq, IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Rat, Mouse samples. Cited in 15 publications.

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Images

Immunoprecipitation - Anti-HDAC2 antibody [Y461] - BSA and Azide free (AB213700), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	ChIC/CUT&RUN-seq	IHC-P	IP	WB	ICC/IF	Flow Cyt (Intra)
Human	Tested	Tested	Tested	Tested	Tested	Tested
Mouse	Expected	Tested	Expected	Tested	Expected	Expected
Rat	Expected	Tested	Expected	Tested	Expected	Expected

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat, Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Rat, Mouse, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

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5 products for Alternative Version

Target data

See full target information HDAC2

Function

Histone deacetylase that catalyzes the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) (PubMed:28497810). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events (By similarity). Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR (PubMed:12724404). Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development (By similarity). Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin (PubMed:16428440, PubMed:28977666). Component of the SIN3B complex that represses transcription and counteracts the histone acetyltransferase activity of EP300 through the recognition H3K27ac marks by PHF12 and the activity of the histone deacetylase HDAC2 (PubMed:37137925). Also deacetylates non-histone targets: deacetylates TSHZ3, thereby regulating its transcriptional repressor activity (PubMed:19343227). May be involved in the transcriptional repression of circadian target genes, such as PER1, mediated by CRY1 through histone deacetylation (By similarity). Involved in MTA1-mediated transcriptional corepression of TFF1 and CDKN1A (PubMed:21965678). In addition to protein deacetylase activity, also acts as a protein-lysine deacylase by recognizing other acyl groups: catalyzes removal of (2E)-butenoyl (crotonyl), lactoyl (lactyl) and 2-hydroxyisobutanoyl (2-hydroxyisobutyryl) acyl groups from lysine residues, leading to protein decrotonylation, delactylation and de-2-hydroxyisobutyrylation, respectively (PubMed:28497810, PubMed:29192674, PubMed:35044827).

Alternative names

Histone deacetylase 2, HD2, Protein deacylase HDAC2, HDAC2

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: Y461
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: Do Not Freeze

Notes

ab213700 is the carrier-free version of Anti-HDAC2 antibody [Y461] ab32117.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

HDAC2 or Histone Deacetylase 2 is an important enzyme that plays a role in chromatin remodeling and gene expression regulation. It removes acetyl groups from histone tails allowing chromatin to condense and silencing gene expression. HDAC2 has a molecular weight of approximately 55 kDa. It is expressed in various tissues including brain heart and skeletal muscles indicating its importance in different physiological areas. HDAC2 proteins are often studied using methods like HDAC2 ELISA and HDAC2 anticuero in research settings.

Biological function summary

HDAC2 is involved in cell cycle regulation and differentiation. It is part of the co-repressor complex and interacts with other proteins such as Sin3 and NuRD to repress transcription. These interactions are vital for maintaining normal cellular functions and ensuring proper response to external signals. This protein is also connected to HEK293T cells which are a common model in scientific studies due to their robust expression of proteins like HDAC2.

Pathways

HDAC2 participates in the regulation of transcriptional activity and cellular stress response. It is an important player in the histone modification pathway interacting with proteins such as HDAC1 and transcriptional regulators like REST. HDAC2 is also linked with the Notch signaling pathway which is essential for cell fate decisions. These pathways highlight its role in both maintaining cellular homeostasis and adapting to changes in the cellular environment.

Associated diseases and disorders

HDAC2 has a significant connection to neurodegenerative diseases and various cancers. Alzheimer's disease for instance shows altered expression levels of HDAC2 affecting synaptic plasticity and memory formation. In cancer HDAC2 interacts with oncogenic proteins such as p53 influencing tumor progression and metastasis. Monitoring HDAC2 levels and activity could provide insights into disease mechanisms and potential therapeutic targets.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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16 product images

Immunoprecipitation - Anti-HDAC2 antibody [Y461] - BSA and Azide free (ab213700)
This data was developed using ab213700, the same antibody clone in a different buffer formulation.
Purified ab213700 at 1:20 dilution (0.5μg) immunoprecipitating HDAC2 in HeLa whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 μg
Lane 2 (+): ab213700 + HeLa whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Synaptophysin antibody [YE269] - Synaptic Marker ab32127 in HeLa whole cell lysate.
VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) (1:5000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: kDa
All lanes: Immunoprecipitation - Anti-HDAC2 antibody [Y461] (Anti-HDAC2 antibody [Y461] ab32117)
Predicted band size: 55 kDa
Western blot - Anti-HDAC2 antibody [Y461] - BSA and Azide free (ab213700)
HRP Anti-HDAC2 antibody [Y461] ab195851 was shown to specifically react with HDAC2 in wild-type HAP1 cells as signal was lost in HDAC2 knockout cells. Wild-type and HDAC2 knockout samples were subjected to SDS-PAGE. HRP Anti-HDAC2 antibody [Y461] ab195851 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (HRP Anti-HDAC2 antibody [Y461] ab195851).
All lanes: Western blot - HRP Anti-HDAC2 antibody [Y461] (HRP Anti-HDAC2 antibody [Y461] ab195851) at 1/10000 dilution
Lane 1: Wild-type HAP1 whole cell lysate at 20 µg
Lane 2: HDAC2 knockout HAP1 whole cell lysate at 20 µg
Predicted band size: 55 kDa
Observed band size: 55 kDa
Exposure time: 30s
Western blot - Anti-HDAC2 antibody [Y461] - BSA and Azide free (ab213700)
All lanes: Western blot - Anti-HDAC2 antibody [Y461] (Anti-HDAC2 antibody [Y461] ab32117) at 1/10000 dilution
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2: Mouse brain lysate
Lane 3: Rat brain lysate
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 55 kDa
Flow Cytometry (Intracellular) - Anti-HDAC2 antibody [Y461] - BSA and Azide free (ab213700)
This data was developed using ab213700, the same antibody clone in a different buffer formulation.
Flow Cytometry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labelling HDAC2 with Purified ab213700 at 1:20 dilution (5 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Immunocytochemistry/ Immunofluorescence - Anti-HDAC2 antibody [Y461] - BSA and Azide free (ab213700)
This data was developed using ab213700, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling HDAC2 with Purified ab213700 at 1:50 dilution (2.1μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC2 antibody [Y461] - BSA and Azide free (ab213700)
This data was developed using Anti-HDAC2 antibody [Y461] ab32117, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat testis tissue sections labeling HDAC2 with Purified Anti-HDAC2 antibody [Y461] ab32117 at 1:1500 dilution (0.071 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC2 antibody [Y461] - BSA and Azide free (ab213700)
This data was developed using Anti-HDAC2 antibody [Y461] ab32117, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse testis tissue sections labeling HDAC2 with Purified Anti-HDAC2 antibody [Y461] ab32117 at 1:1500 dilution (0.071 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC2 antibody [Y461] - BSA and Azide free (ab213700)
This data was developed using Anti-HDAC2 antibody [Y461] ab32117, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue sections labeling HDAC2 with Purified Anti-HDAC2 antibody [Y461] ab32117 at 1:1500 dilution (0.071 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC2 antibody [Y461] - BSA and Azide free (ab213700)
This data was developed using Anti-HDAC2 antibody [Y461] ab32117, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast cancer tissue sections labeling HDAC2 with Purified Anti-HDAC2 antibody [Y461] ab32117 at 1:1500 dilution (0.071 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
Western blot - Anti-HDAC2 antibody [Y461] - BSA and Azide free (ab213700)
This data was generated using the same anti-HDAC2 antibody clone, Y461, in a different buffer formulation (cat# Anti-HDAC2 antibody [Y461] ab32117)
Lane 1: Wild type HAP1 whole cell lysate (20 μg)
Lane 2: HDAC2 knockout HAP1 whole cell lysate (20 μg)
Lane 3: A431 whole cell lysate (20 μg)
Lane 4: Hela whole cell lysate (20 μg)
Lanes 1 - 4: Merged signal (red and green). Green - Anti-HDAC2 antibody [Y461] ab32117 observed at 60 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
Anti-HDAC2 antibody [Y461] ab32117 was shown to specifically react with HDAC2 when HDAC2 knockout samples were used. Wild-type and HDAC2 knockout samples were subjected to SDS-PAGE. Anti-HDAC2 antibody [Y461] ab32117 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/2000 and 1/10000 respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-HDAC2 antibody [Y461] - BSA and Azide free (ab213700)
Predicted band size: 55 kDa
Immunocytochemistry/ Immunofluorescence - Anti-HDAC2 antibody [Y461] - BSA and Azide free (ab213700)
This ICC data was generated using the same anti-HDAC2 antibody clone, Y461, in a different buffer formulation (cat# Anti-HDAC2 antibody [Y461] ab32117).
Anti-HDAC2 antibody [Y461] ab32117 staining HDAC2 in wild-type HAP1 cells (top panel) and HDAC2 knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with Anti-HDAC2 antibody [Y461] ab32117 at 1/250 dilution and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 at 1/250 dilution (shown in pseudocolour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
ChIC/CUT&RUN sequencing - Anti-HDAC2 antibody [Y461] - BSA and Azide free (ab213700)
ChIC/CUT&RUN was performed using a pAG-MNAse at a final concentration of 700 ng/mL, 2 x 10^5 K-562 (Human chronic myelogenous leukemia lymphoblast) cells and 5 µg of Anti-HDAC2 antibody [Y461] ab32117 [Y461] . The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown.

Additional screenshots of mapped reads can be found in the Protocol booklet in the Support and downloads section.

The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

This data was developed using Anti-HDAC2 antibody [Y461] ab32117, the same antibody clone in a different buffer formulation.
Immunocytochemistry/ Immunofluorescence - Anti-HDAC2 antibody [Y461] - BSA and Azide free (ab213700)
This data was developed using Anti-HDAC2 antibody [Y461] ab32117, the same antibody clone in a different buffer formulation.

Immunocytochemistry/ Immunofluorescence analysis of Wild-type HEK293T/HDAC2 KO HEK293T (HDAC2 knockout human embryonic kidney epithelial cell) (Human HDAC2 knockout HEK-293T cell line ab266590) cells labeling HDAC2 with Anti-HDAC2 antibody [Y461] ab32117 at 1/200 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody at 1/1000 dilution (2 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (2.5 µg/ml). DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Confocal image showing nuclear staining in Parental HEK293T cell line. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC2 antibody [Y461] - BSA and Azide free (ab213700)
This data was developed using Anti-HDAC2 antibody [Y461] ab32117, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded fixed (A) Wild-type HEK293T (human embryonic kidney epithelial cell) cell pellet. (B) HDAC2 knockout HEK293T (Human HDAC2 knockout HEK-293T cell line ab266590) cell pellet staining HDAC2 with Anti-HDAC2 antibody [Y461] ab32117 at 1/10000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Counter-staining used was hematoxylin.
Positive staining on (A) Wild-type HEK293T cell pellet, no staining on HDAC2 knockout HEK293T (Human HDAC2 knockout HEK-293T cell line ab266590) cell pellet.
The section was incubated with Anti-HDAC2 antibody [Y461] ab32117 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Western blot - Anti-HDAC2 antibody [Y461] - BSA and Azide free (ab213700)
This data was developed using Anti-HDAC2 antibody [Y461] ab32117, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration: 5% NFDM/TBST
All lanes: Western blot - Anti-HDAC2 antibody [Y461] (Anti-HDAC2 antibody [Y461] ab32117) at 1/1000 dilution
Lane 1: GH3 (rat pituitary epithelial cell) whole cell lysate at 20 µg
Lane 2: L6 (rat skeletal muscle myoblast) whole cell lysate at 20 µg
Lane 3: C6 (rat glial tumor glial cell) whole cell lysate at 20 µg
Lane 4: AR42J (rat pancreatic tumor epithelial cell) whole cell lysate at 20 µg
Lane 5: 2.4G2 (rat B cell lymphoma B lymphocyte) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 55 kDa
Observed band size: 60 kDa
Exposure time: 37s
Western blot - Anti-HDAC2 antibody [Y461] - BSA and Azide free (ab213700)
This data was developed using Anti-HDAC2 antibody [Y461] ab32117, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration: 5% NFDM/TBST
All lanes: Western blot - Anti-HDAC2 antibody [Y461] (Anti-HDAC2 antibody [Y461] ab32117) at 1/1000 dilution
Lane 1: HT-22 (mouse hippocampal neuronal cell) whole cell lysate at 20 µg
Lane 2: SW10 (mouse neuronal Schwann cell) whole cell lysate at 20 µg
Lane 3: bEnd.3 (mouse brain endothelioma) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 55 kDa
Observed band size: 60 kDa
Exposure time: 26s