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AB137704

Anti-HDAC3 antibody

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(14 Publications)

Rabbit Polyclonal HDAC3 antibody. Suitable for IHC-P, IP, ChIP, WB, ICC/IF and reacts with Human, Mouse, Rat, Drosophila melanogaster samples. Cited in 14 publications. Immunogen corresponding to Synthetic Peptide within Human HDAC3 aa 350 to C-terminus.

View Alternative Names

Histone deacetylase 3, HD3, Protein deacetylase HDAC3, Protein deacylase HDAC3, RPD3-2, SMAP45, HDAC3

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-HDAC3 antibody (AB137704)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HDAC3 antibody (AB137704)

SK-N-SH cells stained for HDAC3 (green) using ab137704 at 1/400 dilution in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC3 antibody (AB137704)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC3 antibody (AB137704)

Immunohistochemistry analysis of paraffin-embedded SW480 xenograft labelling FGFR3 with ab137704 at 1/500.

Immunoprecipitation - Anti-HDAC3 antibody (AB137704)
  • IP

Supplier Data

Immunoprecipitation - Anti-HDAC3 antibody (AB137704)

HDAC3 was immunoprecipitated from 1000μg of HEK-293T whole cell lysate with ab137704 at 1/1000 dilution. 10% SDS-PAGE. EasyBlot anti-rabbit IgG was used as secondary antibody.

Lane 1 : 50 μg HEK-293T whole cell lysate/extract.
Lane 2 : Control with 2 μg of preimmune rabbit IgG.
Lane 3 : Immunoprecipitation of HDAC3 protein by 2 μg of HDAC3 antibody.

All lanes:

Immunoprecipitation - Anti-HDAC3 antibody (ab137704)

Predicted band size: 48 kDa

false

ChIP - Anti-HDAC3 antibody (AB137704)
  • ChIP

Supplier Data

ChIP - Anti-HDAC3 antibody (AB137704)

HDAC3 antibody immunoprecipitates HDAC3 protein-DNA in ChIP experiments. ChIP Sample : HEK-293T whole cell lysate/extract

A. 5 μg preimmune rabbit IgG

B. 5 μg of HDAC3 antibody. The precipitated DNA was detected by PCR with primer set targeting to p21 promoter.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Drosophila melanogaster, Mouse, Rat, Human

Applications

ICC/IF, IP, ChIP, WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human HDAC3 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O15379

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HDAC3 or Histone Deacetylase 3 belongs to the class I histone deacetylases and plays an important mechanical role in epigenetic regulation by removing acetyl groups from histone proteins which causes chromatin condensation and gene expression modulation. Often referred to by its alternate name 3g6 HDAC3 has a molecular weight of approximately 49 kDa. The protein expresses broadly in various tissues including the brain liver and heart highlighting its widespread regulatory function across different body systems.
Biological function summary

HDAC3 acts as part of the nuclear receptor corepressor complex engaging with other subunits to modify chromatin structure and gene transcription. This protein influences various cellular processes such as cell cycle regulation and apoptosis. By altering chromatin architecture HDAC3 modulates the expression of numerous genes supporting cellular homeostasis and response to environmental signals.

Pathways

The HDAC3 protein integrates into key signaling networks including the Notch and NF-kB pathways. Within the Notch signaling pathway HDAC3 interacts with proteins like CSL modulating transcriptional responses critical for cell differentiation and developmental processes. In the NF-kB pathway it influences inflammation and immune responses by regulating the transcription of inflammatory genes showcasing its role in mediating complex signaling cascades.

HDAC3 is pivotal in the development and progression of various diseases such as cancer and neurodegenerative disorders. The dysregulation of HDAC3 activity can lead to abnormal cell proliferation and impaired cell death contributing to tumor growth. In neurodegenerative conditions HDAC3 interacts with proteins like tau potentially influencing tau-related pathologies. HDAC3 inhibitors are under investigation for therapeutic approaches to these challenging medical conditions highlighting its relevance in disease management.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Histone deacetylase that catalyzes the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), and some other non-histone substrates (PubMed : 21030595, PubMed : 21444723, PubMed : 23911289, PubMed : 25301942, PubMed : 28167758, PubMed : 28497810, PubMed : 32404892, PubMed : 22230954). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events (PubMed : 23911289). Histone deacetylases act via the formation of large multiprotein complexes, such as N-Cor repressor complex, which activate the histone deacetylase activity (PubMed : 23911289, PubMed : 22230954). Participates in the BCL6 transcriptional repressor activity by deacetylating the H3 'Lys-27' (H3K27) on enhancer elements, antagonizing EP300 acetyltransferase activity and repressing proximal gene expression (PubMed : 23911289). Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation (By similarity). Contributes, together with XBP1 isoform 1, to the activation of NFE2L2-mediated HMOX1 transcription factor gene expression in a PI(3)K/mTORC2/Akt-dependent signaling pathway leading to endothelial cell (EC) survival under disturbed flow/oxidative stress (PubMed : 25190803). Regulates both the transcriptional activation and repression phases of the circadian clock in a deacetylase activity-independent manner (By similarity). During the activation phase, promotes the accumulation of ubiquitinated BMAL1 at the E-boxes and during the repression phase, blocks FBXL3-mediated CRY1/2 ubiquitination and promotes the interaction of CRY1 and BMAL1 (By similarity). The NCOR1-HDAC3 complex regulates the circadian expression of the core clock gene BMAL1 and the genes involved in lipid metabolism in the liver (By similarity). Also functions as a deacetylase for non-histone targets, such as KAT5, MEF2D, MAPK14, RARA and STAT3 (PubMed : 15653507, PubMed : 21030595, PubMed : 21444723, PubMed : 25301942, PubMed : 28167758). Serves as a corepressor of RARA, mediating its deacetylation and repression, leading to inhibition of RARE DNA element binding (PubMed : 28167758). In association with RARA, plays a role in the repression of microRNA-10a and thereby in the inflammatory response (PubMed : 28167758). In addition to protein deacetylase activity, also acts as a protein-lysine deacylase by recognizing other acyl groups : catalyzes removal of (2E)-butenoyl (crotonyl), lactoyl (lactyl), 2-hydroxyisobutanoyl (2-hydroxyisobutyryl) and isonicotinyl acyl groups from lysine residues, leading to protein decrotonylation, delactylation, de-2-hydroxyisobutyrylation and deisonicotinylation, respectively (PubMed : 28497810, PubMed : 29192674, PubMed : 34608293, PubMed : 34545082, PubMed : 35044827). Catalyzes decrotonylation of MAPRE1/EB1 (PubMed : 34608293). Mediates delactylation NBN/NBS1, thereby inhibiting DNA double-strand breaks (DSBs) via homologous recombination (HR) (PubMed : 38961290).
See full target information HDAC3
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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Nature genetics 57:2468-2481 PubMed41044247

2025

Targeting histone H2B acetylated enhanceosomes via p300/CBP degradation in prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Luo,Zhixiang Chen,Yuanyuan Qiao,Jean Ching-Yi Tien,Eleanor Young,Rahul Mannan,Somnath Mahapatra,Rupam Bhattacharyya,Lanbo Xiao,Tongchen He,Sanjana Eyunni,Yuping Zhang,Yang Zheng,Fengyun Su,Xuhong Cao,Rui Wang,Yunhui Cheng,Rithvik Seri,James George,Miriam Shahine,Stephanie J Miner,Matthew G Rees,Melissa M Ronan,Jennifer A Roth,Ulka Vaishampayan,Mi Wang,Shaomeng Wang,Abhijit Parolia,Arul M Chinnaiyan

CNS neuroscience & therapeutics 31:e70471 PubMed40485011

2025

Targeting HDAC3 Suppresses Ferroptosis and Demyelination in White Matter Injury by Restoring PDK4-Mediated Iron Homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Xu,Lisha Ye,Wenfeng Li,Fangming Liu,Tianjiao Wei,Wenhui Gu,Lihua Xu,Mingde Fang,Qianqian Luo,Chuanjie Wu,Guohua Wang

Molecular medicine (Cambridge, Mass.) 30:229 PubMed39580381

2024

Mbnl1-mediated alternative splicing of circMlxipl regulates Rbbp6-involved ChREBP turnover to inhibit lipotoxicity-induced β-cell damage.

Applications

Unspecified application

Species

Unspecified reactive species

Yingying Gong,Meilin Wei,Xiaopei Cao,Changliu Xu,Jiewen Jin,Ling Pei,Yanbing Li,Haipeng Xiao,Liting Wu

Nature communications 15:8044 PubMed39271654

2024

Unbiased screening identifies regulators of cell-cell adhesion and treatment options in pemphigus.

Applications

Unspecified application

Species

Unspecified reactive species

Henriette Franz,Maitreyi Rathod,Aude Zimmermann,Chiara Stüdle,Vivien Beyersdorfer,Karen Leal-Fischer,Pauline Hanns,Tomás Cunha,Dario Didona,Michael Hertl,Marion Scheibe,Falk Butter,Enno Schmidt,Volker Spindler

International journal of immunogenetics 50:281-290 PubMed37700429

2023

HDAC3-mediated lncRNA ZFAS1 inhibited IL-13-induced secretion of proinflammatory cytokines in nasal epithelial cells by regulating the miR-7-5p/SIRT1 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jiabin Zhan,Rui Li,Yi Ye,Jing Zheng,Gang Wang,Jinli Wu,Xin Wei,Min Zeng

Arteriosclerosis, thrombosis, and vascular biology 43:1900-1920 PubMed37589142

2023

Causal Role for Neutrophil Elastase in Thoracic Aortic Dissection in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Mei Yang,Xinmiao Zhou,Stuart W A Pearce,Zhisheng Yang,Qishan Chen,Kaiyuan Niu,Chenxin Liu,Jun Luo,Dan Li,Yue Shao,Cheng Zhang,Dan Chen,Qingchen Wu,Pedro R Cutillas,Lin Zhao,Qingzhong Xiao,Li Zhang

BMC ophthalmology 23:302 PubMed37415101

2023

Apigenin inhibits angiogenesis in retinal microvascular endothelial cells through regulating of the miR-140-5p/HDAC3-mediated PTEN/PI3K/AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chaojun Fu,Jun Peng,Yanjun Ling,Hongqing Zhao,Yongwang Zhao,Xiuli Zhang,Min Ai,Qinghua Peng,Yuhui Qin

iScience 26:107158 PubMed37404376

2023

HDAC3 promotes macrophage pyroptosis via regulating histone deacetylation in acute lung injury.

Applications

Unspecified application

Species

Unspecified reactive species

Ning Li,Bohao Liu,Ruyuan He,Guorui Li,Rui Xiong,Tinglv Fu,Donghang Li,Chenzhen Xu,Bo Wang,Qing Geng

Molecular carcinogenesis 62:754-770 PubMed36920044

2023

Circular RNA ACACA negatively regulated p53-modulated mevalonate pathway to promote colorectal tumorigenesis via regulating miR-193a/b-3p/HDAC3 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Fengjiao He,Qiong Liu,Huan Liu,Qian Pei,Hong Zhu

Cancers 14: PubMed36230720

2022

CREPT Disarms the Inhibitory Activity of HDAC1 on Oncogene Expression to Promote Tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yajun Cao,Bobin Ning,Ye Tian,Tingwei Lan,Yunxiang Chu,Fangli Ren,Yinyin Wang,Qingyu Meng,Jun Li,Baoqing Jia,Zhijie Chang
View all publications
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Product promise

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