AB76295

Anti-HDAC3 antibody [EP462Y]

20ul selling size
RabMAb
Recombinant
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(9 Publications)

Rabbit Recombinant Monoclonal HDAC3 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 9 publications.

View Alternative Names

Histone deacetylase 3, HD3, Protein deacetylase HDAC3, Protein deacylase HDAC3, RPD3-2, SMAP45, HDAC3

8 Images

Immunocytochemistry/ Immunofluorescence - Anti-HDAC3 antibody [EP462Y] (AB76295)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HDAC3 antibody [EP462Y] (AB76295)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling HDAC3 with Purified ab76295 at 1 : 50 dilution (2.1 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1 : 200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488 ab150077) was used as the secondary antibody at 1 : 1000 (2 μg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC3 antibody [EP462Y] (AB76295)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC3 antibody [EP462Y] (AB76295)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung carcinoma tissue sections labeling HDAC3 with Purified ab76295 at 1 : 100 dilution (1.03 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Flow Cytometry (Intracellular) - Anti-HDAC3 antibody [EP462Y] (AB76295)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-HDAC3 antibody [EP462Y] (AB76295)

Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling HDAC3 with Purified ab76295 at 1/20 dilution (10μg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluorr^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC3 antibody [EP462Y] (AB76295)

ab76295 at a 1/100-1/250 dilution staining human HDAC3 in breast carcinoma by Immunohistochemistry Formalin/PFA-fixed paraffin-embedded tissue.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC3 antibody [EP462Y] (AB76295)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat kidney tissue sections labeling HDAC3 with Purified ab76295 at 1 : 100 dilution (1.03 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC3 antibody [EP462Y] (AB76295)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cerebrum tissue sections labeling HDAC3 with Purified ab76295 at 1 : 100 dilution (1.03 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Lab

Western blot - Anti-HDAC3 antibody [EP462Y] (AB76295)

All lanes:

Western blot - Anti-HDAC3 antibody [EP462Y] (ab76295) at 1/2000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

Jurkat cell lysate at 20 µg

Lane 3:

HepG2 cell lysate at 20 µg

Lane 4:

NIH/3T3 cell lysate at 20 µg

Lane 5:

PC-12 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 48 kDa

Observed band size: 49 kDa

false

Unknown

Western blot - Anti-HDAC3 antibody [EP462Y] (AB76295)

All lanes:

Western blot - Anti-HDAC3 antibody [EP462Y] (ab76295) at 1/2000 dilution

All lanes:

HeLa cell lysate at 10 µg

Secondary

All lanes:

goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 48 kDa

Observed band size: 49 kDa

false

Carrier free

Anti-HDAC3 antibody [EP462Y] - BSA and Azide free
660 APC

APC Anti-HDAC3 antibody [EP462Y]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-HDAC3 antibody [EP462Y]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-HDAC3 antibody [EP462Y]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-HDAC3 antibody [EP462Y]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-HDAC3 antibody [EP462Y]
HRP

HRP Anti-HDAC3 antibody [EP462Y]
578 PE

PE Anti-HDAC3 antibody [EP462Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP462Y

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, IHC-P, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "For unpurified use at 1/100", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/20", "FlowCytIntra-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 99% Tissue culture supernatant, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HDAC3 or Histone Deacetylase 3 belongs to the class I histone deacetylases and plays an important mechanical role in epigenetic regulation by removing acetyl groups from histone proteins which causes chromatin condensation and gene expression modulation. Often referred to by its alternate name 3g6 HDAC3 has a molecular weight of approximately 49 kDa. The protein expresses broadly in various tissues including the brain liver and heart highlighting its widespread regulatory function across different body systems.

Biological function summary

HDAC3 acts as part of the nuclear receptor corepressor complex engaging with other subunits to modify chromatin structure and gene transcription. This protein influences various cellular processes such as cell cycle regulation and apoptosis. By altering chromatin architecture HDAC3 modulates the expression of numerous genes supporting cellular homeostasis and response to environmental signals.

Pathways

The HDAC3 protein integrates into key signaling networks including the Notch and NF-kB pathways. Within the Notch signaling pathway HDAC3 interacts with proteins like CSL modulating transcriptional responses critical for cell differentiation and developmental processes. In the NF-kB pathway it influences inflammation and immune responses by regulating the transcription of inflammatory genes showcasing its role in mediating complex signaling cascades.

HDAC3 is pivotal in the development and progression of various diseases such as cancer and neurodegenerative disorders. The dysregulation of HDAC3 activity can lead to abnormal cell proliferation and impaired cell death contributing to tumor growth. In neurodegenerative conditions HDAC3 interacts with proteins like tau potentially influencing tau-related pathologies. HDAC3 inhibitors are under investigation for therapeutic approaches to these challenging medical conditions highlighting its relevance in disease management.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Histone deacetylase that catalyzes the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), and some other non-histone substrates (PubMed : 21030595, PubMed : 21444723, PubMed : 23911289, PubMed : 25301942, PubMed : 28167758, PubMed : 28497810, PubMed : 32404892, PubMed : 22230954). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events (PubMed : 23911289). Histone deacetylases act via the formation of large multiprotein complexes, such as N-Cor repressor complex, which activate the histone deacetylase activity (PubMed : 23911289, PubMed : 22230954). Participates in the BCL6 transcriptional repressor activity by deacetylating the H3 'Lys-27' (H3K27) on enhancer elements, antagonizing EP300 acetyltransferase activity and repressing proximal gene expression (PubMed : 23911289). Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation (By similarity). Contributes, together with XBP1 isoform 1, to the activation of NFE2L2-mediated HMOX1 transcription factor gene expression in a PI(3)K/mTORC2/Akt-dependent signaling pathway leading to endothelial cell (EC) survival under disturbed flow/oxidative stress (PubMed : 25190803). Regulates both the transcriptional activation and repression phases of the circadian clock in a deacetylase activity-independent manner (By similarity). During the activation phase, promotes the accumulation of ubiquitinated BMAL1 at the E-boxes and during the repression phase, blocks FBXL3-mediated CRY1/2 ubiquitination and promotes the interaction of CRY1 and BMAL1 (By similarity). The NCOR1-HDAC3 complex regulates the circadian expression of the core clock gene BMAL1 and the genes involved in lipid metabolism in the liver (By similarity). Also functions as a deacetylase for non-histone targets, such as KAT5, MEF2D, MAPK14, RARA and STAT3 (PubMed : 15653507, PubMed : 21030595, PubMed : 21444723, PubMed : 25301942, PubMed : 28167758). Serves as a corepressor of RARA, mediating its deacetylation and repression, leading to inhibition of RARE DNA element binding (PubMed : 28167758). In association with RARA, plays a role in the repression of microRNA-10a and thereby in the inflammatory response (PubMed : 28167758). In addition to protein deacetylase activity, also acts as a protein-lysine deacylase by recognizing other acyl groups : catalyzes removal of (2E)-butenoyl (crotonyl), lactoyl (lactyl), 2-hydroxyisobutanoyl (2-hydroxyisobutyryl) and isonicotinyl acyl groups from lysine residues, leading to protein decrotonylation, delactylation, de-2-hydroxyisobutyrylation and deisonicotinylation, respectively (PubMed : 28497810, PubMed : 29192674, PubMed : 34608293, PubMed : 34545082, PubMed : 35044827). Catalyzes decrotonylation of MAPRE1/EB1 (PubMed : 34608293). Mediates delactylation NBN/NBS1, thereby inhibiting DNA double-strand breaks (DSBs) via homologous recombination (HR) (PubMed : 38961290).

See full target information HDAC3

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

eLife 13: PubMed39373630

2024

Super-enhancer-driven ZFP36L1 promotes PD-L1 expression in infiltrative gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xujin Wei,Jie Liu,Jia Cheng,Wangyu Cai,Wen Xie,Kang Wang,Lingyun Lin,Jingjing Hou,Jianchun Cai,Huiqin Zhuo

Cell metabolism 36:1764-1778.e9 PubMed38889724

2024

Short-term cold exposure induces persistent epigenomic memory in brown fat.

Applications

Unspecified application

Species

Unspecified reactive species

Shin-Ichi Inoue,Matthew J Emmett,Hee-Woong Lim,Mohit Midha,Hannah J Richter,Isaac J Celwyn,Rashid Mehmood,Maria Chondronikola,Samuel Klein,Amy K Hauck,Mitchell A Lazar

mBio 14:e0069623 PubMed37382436

2023

Mus musculus papillomavirus 1 E8^E2 represses expression of late protein E4 in basal-like keratinocytes via NCoR/SMRT-HDAC3 co-repressor complexes to enable wart formation .

Applications

Unspecified application

Species

Unspecified reactive species

Franziska Kuehner,Margaret Wong,Elke Straub,John Doorbar,Thomas Iftner,Richard B S Roden,Frank Stubenrauch

Annals of translational medicine 10:927 PubMed36172099

2022

Protective role of muscones on astrocytes under a mechanical-chemical damage model.

Applications

Unspecified application

Species

Unspecified reactive species

Rui-Fu Li,Fei Gui,Chao Yu,Yuan-Meng Luo,Liang Guo

The Journal of experimental medicine 219: PubMed35977109

2022

A dysregulated sebum-microbial metabolite-IL-33 axis initiates skin inflammation in atopic dermatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuoqiong Qiu,Zhenlai Zhu,Xiaochun Liu,Baichao Chen,Huibin Yin,Chaoying Gu,Xiaokai Fang,Ronghui Zhu,Tianze Yu,Wenli Mi,Hong Zhou,Yufeng Zhou,Xu Yao,Wei Li

Scientific reports 7:6082 PubMed28729730

2017

A selective inhibitor of histone deacetylase 3 prevents cognitive deficits and suppresses striatal CAG repeat expansions in Huntington's disease mice.

Applications

Species

Mouse

Nuria Suelves,Lucy Kirkham-McCarthy,Robert S Lahue,Silvia Ginés

Nature 546:544-548 PubMed28614293

2017

Histone deacetylase 3 prepares brown adipose tissue for acute thermogenic challenge.

Applications

Species

Mouse

Matthew J Emmett,Hee-Woong Lim,Jennifer Jager,Hannah J Richter,Marine Adlanmerini,Lindsey C Peed,Erika R Briggs,David J Steger,Tao Ma,Carrie A Sims,Joseph A Baur,Liming Pei,Kyoung-Jae Won,Patrick Seale,Zachary Gerhart-Hines,Mitchell A Lazar

Human molecular genetics 24:1127-41 PubMed25305081

2014

PINK1 positively regulates HDAC3 to suppress dopaminergic neuronal cell death.

Applications

Unspecified application

Species

Unspecified reactive species

Hyo-Kyoung Choi,Youngsok Choi,HeeBum Kang,Eun-Jin Lim,Soo-Yeon Park,Hyun-Seob Lee,Ji-Min Park,Jisook Moon,Yoon-Jung Kim,Insup Choi,Eun-Hye Joe,Kyung-Chul Choi,Ho-Geun Yoon

Pharmaceuticals (Basel, Switzerland) 4:1183-1195 PubMed22582024

2012

Histone Deacetylase Inhibitors and Mithramycin A Impact a Similar Neuroprotective Pathway at a Crossroad between Cancer and Neurodegeneration.

Applications

Species

Rat

Sama F Sleiman,Jill Berlin,Manuela Basso,Saravanan S Karuppagounder,Jürgen Rohr,Rajiv R Ratan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HDAC3 antibody [EP462Y]

Immunocytochemistry/ Immunofluorescence - Anti-HDAC3 antibody [EP462Y] (AB76295)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC3 antibody [EP462Y] (AB76295)

Flow Cytometry (Intracellular) - Anti-HDAC3 antibody [EP462Y] (AB76295)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC3 antibody [EP462Y] (AB76295)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC3 antibody [EP462Y] (AB76295)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC3 antibody [EP462Y] (AB76295)

Western blot - Anti-HDAC3 antibody [EP462Y] (AB76295)

Western blot - Anti-HDAC3 antibody [EP462Y] (AB76295)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

Product promise