Anti-HDAC3 antibody [EP462Y] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HDAC3 antibody [EP462Y] - BSA and Azide free (AB247459)

This data was developed using ab76295, the same antibody clone in a different buffer formulation.

Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling HDAC3 with Purified ab76295 at 1 : 50 dilution (2.1 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1 : 200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 μg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC3 antibody [EP462Y] - BSA and Azide free (AB247459)

This data was developed using ab76295, the same antibody clone in a different buffer formulation.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat kidney tissue sections labeling HDAC3 with Purified ab76295 at 1 : 100 dilution (1.03 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC3 antibody [EP462Y] - BSA and Azide free (AB247459)

This data was developed using ab76295, the same antibody clone in a different buffer formulation.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung carcinoma tissue sections labeling HDAC3 with Purified ab76295 at 1 : 100 dilution (1.03 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-HDAC3 antibody [EP462Y] - BSA and Azide free (AB247459)

This data was developed using ab76295, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling HDAC3 with Purified ab76295 at 1/20 dilution (10 μg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC3 antibody [EP462Y] - BSA and Azide free (AB247459)

This data was developed using ab76295, the same antibody clone in a different buffer formulation.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cerebrum tissue sections labeling HDAC3 with Purified ab76295 at 1 : 100 dilution (1.03 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC3 antibody [EP462Y] - BSA and Azide free (AB247459)

This data was developed using ab76295, the same antibody clone in a different buffer formulation.

ab76295, at a 1/100-1/250 dilution, staining human HDAC3 in breast carcinoma, by Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

• WB

Lab

Western blot - Anti-HDAC3 antibody [EP462Y] - BSA and Azide free (AB247459)

This data was developed using ab76295, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-HDAC3 antibody [EP462Y] (<a href='/en-us/products/primary-antibodies/hdac3-antibody-ep462y-ab76295'>ab76295</a>) at 1/2000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

Jurkat cell lysate at 20 µg

Lane 3:

HepG2 cell lysate at 20 µg

Lane 4:

NIH/3T3 cell lysate at 20 µg

Lane 5:

PC-12 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 48 kDa

Observed band size: 49 kDa

false

• WB

Unknown

Western blot - Anti-HDAC3 antibody [EP462Y] - BSA and Azide free (AB247459)

This data was developed using ab76295, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-HDAC3 antibody [EP462Y] (<a href='/en-us/products/primary-antibodies/hdac3-antibody-ep462y-ab76295'>ab76295</a>) at 1/2000 dilution

All lanes:

HeLa cell lysate at 10 µg

Secondary

All lanes:

goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 48 kDa

Observed band size: 49 kDa

false