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Anti-HDAC3 antibody [Y415] (ab32369) is a rabbit monoclonal antibody that is used to detect HDAC3 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

-Over 60 publications

-Over 60 publications


Images

Flow Cytometry (Intracellular) - Anti-HDAC3 antibody [Y415] (AB32369), expandable thumbnail
  • Western blot - Anti-HDAC3 antibody [Y415] (AB32369), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-HDAC3 antibody [Y415] (AB32369), expandable thumbnail
  • Immunoprecipitation - Anti-HDAC3 antibody [Y415] (AB32369), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC3 antibody [Y415] (AB32369), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.12% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Expected
Expected
Tested
Expected
Expected
Rat
Expected
Expected
Tested
Expected
Expected

Tested
Tested

Species
Human
Dilution info
1/50
Notes

For unpurified use at 1/250 - 1/500.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/20 - 1/50
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/5000 - 1/10000
Notes

-

Species
Rat
Dilution info
1/5000 - 1/10000
Notes

-

Species
Human
Dilution info
1/5000 - 1/10000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/250 - 1/500
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

7 products for Alternative Product

Target data

Function

Histone deacetylase that catalyzes the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), and some other non-histone substrates (PubMed:21030595, PubMed:21444723, PubMed:23911289, PubMed:25301942, PubMed:28167758, PubMed:28497810, PubMed:32404892, PubMed:22230954). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events (PubMed:23911289). Histone deacetylases act via the formation of large multiprotein complexes, such as N-Cor repressor complex, which activate the histone deacetylase activity (PubMed:23911289, PubMed:22230954). Participates in the BCL6 transcriptional repressor activity by deacetylating the H3 'Lys-27' (H3K27) on enhancer elements, antagonizing EP300 acetyltransferase activity and repressing proximal gene expression (PubMed:23911289). Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation (By similarity). Contributes, together with XBP1 isoform 1, to the activation of NFE2L2-mediated HMOX1 transcription factor gene expression in a PI(3)K/mTORC2/Akt-dependent signaling pathway leading to endothelial cell (EC) survival under disturbed flow/oxidative stress (PubMed:25190803). Regulates both the transcriptional activation and repression phases of the circadian clock in a deacetylase activity-independent manner (By similarity). During the activation phase, promotes the accumulation of ubiquitinated BMAL1 at the E-boxes and during the repression phase, blocks FBXL3-mediated CRY1/2 ubiquitination and promotes the interaction of CRY1 and BMAL1 (By similarity). The NCOR1-HDAC3 complex regulates the circadian expression of the core clock gene BMAL1 and the genes involved in lipid metabolism in the liver (By similarity). Also functions as a deacetylase for non-histone targets, such as KAT5, MEF2D, MAPK14, RARA and STAT3 (PubMed:15653507, PubMed:21030595, PubMed:21444723, PubMed:25301942, PubMed:28167758). Serves as a corepressor of RARA, mediating its deacetylation and repression, leading to inhibition of RARE DNA element binding (PubMed:28167758). In association with RARA, plays a role in the repression of microRNA-10a and thereby in the inflammatory response (PubMed:28167758). In addition to protein deacetylase activity, also acts as a protein-lysine deacylase by recognizing other acyl groups: catalyzes removal of (2E)-butenoyl (crotonyl), lactoyl (lactyl) and 2-hydroxyisobutanoyl (2-hydroxyisobutyryl) acyl groups from lysine residues, leading to protein decrotonylation, delactylation and de-2-hydroxyisobutyrylation, respectively (PubMed:28497810, PubMed:29192674, PubMed:34608293, PubMed:35044827). Catalyzes decrotonylation of MAPRE1/EB1 (PubMed:34608293). Mediates delactylation NBN/NBS1, thereby inhibiting DNA double-strand breaks (DSBs) via homologous recombination (HR) (PubMed:38961290).

Alternative names

Histone deacetylase 3, HD3, Protein deacetylase HDAC3, Protein deacylase HDAC3, RPD3-2, SMAP45, HDAC3

Recommended products

Anti-HDAC3 antibody [Y415] (ab32369) is a rabbit monoclonal antibody that is used to detect HDAC3 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

-Over 60 publications

-Over 60 publications

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
Y415
Purification technique
Affinity purification Protein A
Specificity

This antibody may detect splice isoform 2 (RPD3-2A).

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What is this antibody validated in?


Anti-HDAC3 antibody [Y415] (ab32369) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), in Human, Mouse, Rat samples.

What is the molecular weight of HDAC3?


Anti-HDAC3 [Y415] (ab32369) specifically detects a band for HDAC3 (UniProt: O15379) at a molecular weight of 49kDa.

Recommended positive controls


K562 cell lysate; HeLa cells; human ovary carcinoma

Trusted by the scientific community


Anti-HDAC3 [Y415] (ab32369) was first used in a scientific publication in 2006 and has been cited over 60 times in peer-reviewed journals.

Reviewed by scientists


Anti-HDAC3 [Y415] (ab32369) has over 5 independent reviews from customers.

Trial sizes available!


Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.Other related products


We have a range of other formats of antibody clone [Y415] also available for your convenience:
ab32369, HRP - HRP Anti-HDAC3 antibody [Y415] ab208356, Carrier free - Anti-HDAC3 antibody [Y415] - BSA and Azide free ab219376, APC - APC Anti-HDAC3 antibody [Y415] ab310854, PE - PE Anti-HDAC3 antibody [Y415] ab310923, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-HDAC3 antibody [Y415] ab310969, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-HDAC3 antibody [Y415] ab311086, Alexa Fluor® 594 - Alexa Fluor® 594 Anti-HDAC3 antibody [Y415] ab311683, Alexa Fluor® 568 - Alexa Fluor® 568 Anti-HDAC3 antibody [Y415] ab312958, Alexa Fluor® 555 - Alexa Fluor® 555 Anti-HDAC3 antibody [Y415] ab313167, Alexa Fluor® 750 - Alexa Fluor® 750 Anti-HDAC3 antibody [Y415] ab321594



Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

HDAC3 or Histone Deacetylase 3 belongs to the class I histone deacetylases and plays an important mechanical role in epigenetic regulation by removing acetyl groups from histone proteins which causes chromatin condensation and gene expression modulation. Often referred to by its alternate name 3g6 HDAC3 has a molecular weight of approximately 49 kDa. The protein expresses broadly in various tissues including the brain liver and heart highlighting its widespread regulatory function across different body systems.

Biological function summary

HDAC3 acts as part of the nuclear receptor corepressor complex engaging with other subunits to modify chromatin structure and gene transcription. This protein influences various cellular processes such as cell cycle regulation and apoptosis. By altering chromatin architecture HDAC3 modulates the expression of numerous genes supporting cellular homeostasis and response to environmental signals.

Pathways

The HDAC3 protein integrates into key signaling networks including the Notch and NF-kB pathways. Within the Notch signaling pathway HDAC3 interacts with proteins like CSL modulating transcriptional responses critical for cell differentiation and developmental processes. In the NF-kB pathway it influences inflammation and immune responses by regulating the transcription of inflammatory genes showcasing its role in mediating complex signaling cascades.

Associated diseases and disorders

HDAC3 is pivotal in the development and progression of various diseases such as cancer and neurodegenerative disorders. The dysregulation of HDAC3 activity can lead to abnormal cell proliferation and impaired cell death contributing to tumor growth. In neurodegenerative conditions HDAC3 interacts with proteins like tau potentially influencing tau-related pathologies. HDAC3 inhibitors are under investigation for therapeutic approaches to these challenging medical conditions highlighting its relevance in disease management.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Flow Cytometry (Intracellular) - Anti-HDAC3 antibody [Y415] (ab32369), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-HDAC3 antibody [Y415] (ab32369)

    Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling HDAC3 with purified ab32369 at 1/30 dilution (10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr®488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • Western blot - Anti-HDAC3 antibody [Y415] (ab32369), expandable thumbnail

    Western blot - Anti-HDAC3 antibody [Y415] (ab32369)

    Blocking/Diluting buffer 5% NFDM/TBST

    All lanes: Western blot - Anti-HDAC3 antibody [Y415] (ab32369) at 1/5000 dilution

    Lane 1: K562 (human chronic myelogenous leukemia) whole cell lysate at 20 µg

    Lane 2: HeLa (human cervix adenocarcinoma) whole cell lysate at 20 µg

    Lane 3: NIH/3T3 (mouse embryo) whole cell lysate at 20 µg

    Lane 4: C6 (rat glioma) whole cell lysate at 20 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size: 48 kDa

    Observed band size: 49 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-HDAC3 antibody [Y415] (ab32369), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-HDAC3 antibody [Y415] (ab32369)

    Immunocytochemistry/Immunofluorescence analysis of K562 cells labelling HDAC3 with purified ab32369 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilized using 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, Alexa Fluor®488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were co-stained with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, a mouse anti-tubulin antibody (1/1000) using Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor®594-conjugated goat anti-mouse IgG (1/1000) as the secondary. Nuclei were counterstained with DAPI (blue).

    For negative control 1, rabbit primary antibody and anti-mouse secondary antibody (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) were used and for negative control 2, mouse primary antibody (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) and anti-rabbit secondary antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) were used.

  • Immunoprecipitation - Anti-HDAC3 antibody [Y415] (ab32369), expandable thumbnail

    Immunoprecipitation - Anti-HDAC3 antibody [Y415] (ab32369)

    Purified ab32369 at 1/20 immunoprecipitating HDAC3 in K562 whole cell lysate observed at 49 KDa (lanes 1 and 2).

    Lane 1 (input): K562 whole cell lysate 10ug

    Lane 2 (+): ab32369 + K562 whole cell lysate.

    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab32369 in K562 whole cell lysate

    The secondary antibody used was VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) for detection at dilution of 1/1000.

    Blocking/Diluting buffer 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-HDAC3 antibody [Y415] (ab32369)

    Predicted band size: 48 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC3 antibody [Y415] (ab32369), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC3 antibody [Y415] (ab32369)

    Immunohistochemical analysis of paraffin-embedded human endometrial carcinoma sections labelling HDAC3 with purified ab32369 at dilution of 1:50. The secondary antibody used was Goat Anti-Rabbit IgG H&L (HRP) ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

  • Western blot - Anti-HDAC3 antibody [Y415] (ab32369), expandable thumbnail

    Western blot - Anti-HDAC3 antibody [Y415] (ab32369)

    All lanes: Western blot - Anti-HDAC3 antibody [Y415] (ab32369) at 1/10000 dilution

    All lanes: K562 cell lysate

    Predicted band size: 48 kDa

    Observed band size: 49 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC3 antibody [Y415] (ab32369), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC3 antibody [Y415] (ab32369)

    Paraffin-embedded human ovary carcinoma
    ab32369 at 1/250 dilution

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

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Product protocols

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