Anti-HDAC3 antibody [Y415] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC3 antibody [Y415] - BSA and Azide free (AB219376)

Immunohistochemical analysis of paraffin-embedded human endometrial carcinoma sections labelling HDAC3 with purified ab32369 at dilution of 1 : 50. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32369).

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-HDAC3 antibody [Y415] - BSA and Azide free (AB219376)

Immunocytochemistry/Immunofluorescence analysis of K562 cells labelling HDAC3 with purified ab32369 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilized using 0.1% Triton X-100. ab150077, Alexa Fluor^®488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were co-stained with ab7291, a mouse anti-tubulin antibody (1/1000) using ab150120, an Alexa Fluor^®594-conjugated goat anti-mouse IgG (1/1000) as the secondary. Nuclei were counterstained with DAPI (blue).

For negative control 1, rabbit primary antibody and anti-mouse secondary antibody (ab150120) were used and for negative control 2, mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077) were used.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32369).

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-HDAC3 antibody [Y415] - BSA and Azide free (AB219376)

Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling HDAC3 with purified ab32369 at 1/30 dilution (10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^®488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32369).

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-HDAC3 antibody [Y415] - BSA and Azide free (AB219376)

Immunofluorescent staining of HeLa cells
using ab32369 at 1/250 dilutionThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32369).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC3 antibody [Y415] - BSA and Azide free (AB219376)

Paraffin-embedded human ovary carcinoma
ab32369 at 1/250 dilution

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32369).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IP

Lab

Immunoprecipitation - Anti-HDAC3 antibody [Y415] - BSA and Azide free (AB219376)

Purified ab32369 at 1/20 immunoprecipitating HDAC3 in K562 whole cell lysate observed at 49 KDa (lanes 1 and 2).

Lane 1 (input) : K562 whole cell lysate 10ug

Lane 2 (+) : ab32369 + K562 whole cell lysate.

Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab32369 in K562 whole cell lysate

The Detection Reagent used was VeriBlot for IP (HRP) (ab131366) at dilution of 1/1000.

Blocking/Diluting buffer 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32369).

All lanes:

Immunoprecipitation - Anti-HDAC3 antibody [Y415] (<a href='/en-us/products/primary-antibodies/hdac3-antibody-y415-ab32369'>ab32369</a>)

Predicted band size: 48 kDa

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