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AB131524

Anti-HDAC4 + 5 + 9 antibody

4

(2 Reviews)

|

(3 Publications)

Rabbit Polyclonal HDAC4 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human HDAC4 aa 200-250.

View Alternative Names

KIAA0288, HDAC4, Histone deacetylase 4, HD4, HDAC7, HDAC7B, HDRP, KIAA0744, MITR, HDAC9, Histone deacetylase 9, HD9, Histone deacetylase 7B, Histone deacetylase-related protein, MEF2-interacting transcription repressor MITR, HD7, HD7b, KIAA0600, HDAC5, Histone deacetylase 5, HD5, Antigen NY-CO-9

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC4 + 5 + 9 antibody (AB131524)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC4 + 5 + 9 antibody (AB131524)

Immunohistochemical analysis of paraffin embedded Human lung carcinoma tissue labelled with ab131524 at 1/50 dilution (left) or the same antibody preincubated with blocking peptide (right).

Immunocytochemistry/ Immunofluorescence - Anti-HDAC4 + 5 + 9 antibody (AB131524)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-HDAC4 + 5 + 9 antibody (AB131524)

Immunofluorescence analysis of methanol-fixed HeLa cells labelled with ab131524 at 1/100 dilution.

Western blot - Anti-HDAC4 + 5 + 9 antibody (AB131524)
  • WB

Unknown

Western blot - Anti-HDAC4 + 5 + 9 antibody (AB131524)

All lanes:

Western blot - Anti-HDAC4 + 5 + 9 antibody (ab131524) at 1/500 dilution

Lane 1:

293 cell lysate

Lane 2:

HeLa cell lysate

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Synthetic Peptide within Human HDAC4 aa 200-250. The exact immunogen used to generate this antibody is proprietary information.

P56524

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/200", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/100 - 1/200", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HDAC4 HDAC5 and HDAC9 are types of histone deacetylases abbreviated as HDACs. These enzymes remove acetyl groups from histone and non-histone proteins affecting gene expression by changing chromatin structure. HDAC4 has a molecular weight of about 140 kDa. HDAC4 and HDAC5 are highly expressed in the brain heart and skeletal muscle while HDAC9 is more abundant in the heart and skeletal muscle. They act as transcriptional repressors by interacting with various corepressor complexes.
Biological function summary

Histone deacetylases 4 5 and 9 influence gene expression and cellular differentiation. These proteins often form part of larger corepressor complexes such as the N-CoR and SMRT complexes allowing them to modulate transcriptional activity. They play significant roles in muscle differentiation and neural development interacting with transcription factors like MEF2 to regulate various genes involved in these processes.

Pathways

Histone deacetylases like HDAC4 5 and 9 are critical components of signaling pathways related to calcium/calmodulin-dependent calcineurin signal transduction. This pathway influences muscle development and function where they interact with MEF2 family transcription factors to control gene expression. Additionally they are involved in the regulation of the circadian rhythm through their role in the molecular clockwork partnering with proteins like CLOCK and BMAL1.

Alterations in HDAC4 5 and 9 levels or activity have connections to cardiovascular diseases and certain cancers. Dysfunctional HDAC activity can lead to hypertrophy in cardiac cells affecting heart function. Cancer progression often involves aberrant histone deacetylation with HDACs modulating oncogenic transcription factors. These HDACs closely interact with cancer-related proteins like MYC and p53 influencing tumor suppression or progression depending on cellular context.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. Deacetylates HSPA1A and HSPA1B at 'Lys-77' leading to their preferential binding to co-chaperone STUB1 (PubMed : 27708256).
See full target information HDAC4

Additional targets

HDAC9,HDAC5
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 43:114054 PubMed38578832

2024

14-3-3 binding motif phosphorylation disrupts Hdac4-organized condensates to stimulate cardiac reprogramming.

Applications

Unspecified application

Species

Unspecified reactive species

Liu Liu,Ienglam Lei,Shuo Tian,Wenbin Gao,Yijing Guo,Zhaokai Li,Ziad Sabry,Paul Tang,Y Eugene Chen,Zhong Wang

eLife 12: PubMed37902809

2023

The PMA phorbol ester tumor promoter increases canonical Wnt signaling via macropinocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Nydia Tejeda-Munoz,Yagmur Azbazdar,Julia Monka,Grace Binder,Alex Dayrit,Raul Ayala,Neil O'Brien,Edward M De Robertis

ChemMedChem 14:1096-1107 PubMed30921497

2019

Structurally Diverse Histone Deacetylase Photoreactive Probes: Design, Synthesis, and Photolabeling Studies in Live Cells and Tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Shaimaa M Aboukhatwa,Thomas W Hanigan,Taha Y Taha,Jayaprakash Neerasa,Rajeev Ranjan,Eman E El-Bastawissy,Mohamed A Elkersh,Tarek F El-Moselhy,Jonna Frasor,Nadim Mahmud,Alan McLachlan,Pavel A Petukhov
View all publications
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Product promise

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