Rabbit Recombinant Monoclonal HDAC4 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples.
Images
![Western blot - Anti-HDAC4 + 5 + 9 antibody [EPR19010-77] (AB195241), expandable thumbnail](https://content.abcam.com/products/images/hdac4-5-9-antibody-epr19010-77-ab195241--western-blot-img167589.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-HDAC4 + 5 + 9 antibody [EPR19010-77] (AB195241)")
![Western blot - Anti-HDAC4 + 5 + 9 antibody [EPR19010-77] (AB195241), expandable thumbnail](https://content.abcam.com/products/images/hdac4-5-9-antibody-epr19010-77-ab195241--western-blot-img167206.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-HDAC4 + 5 + 9 antibody [EPR19010-77] (AB195241)")
![Western blot - Anti-HDAC4 + 5 + 9 antibody [EPR19010-77] (AB195241), expandable thumbnail](https://content.abcam.com/products/images/hdac4-5-9-antibody-epr19010-77-ab195241--western-blot-img174153.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-HDAC4 + 5 + 9 antibody [EPR19010-77] (AB195241)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC4 + 5 + 9 antibody [EPR19010-77] (AB195241), expandable thumbnail](https://content.abcam.com/products/images/hdac4-5-9-antibody-epr19010-77-ab195241--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img182545.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC4 + 5 + 9 antibody [EPR19010-77] (AB195241)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC4 + 5 + 9 antibody [EPR19010-77] (AB195241), expandable thumbnail](https://content.abcam.com/products/images/hdac4-5-9-antibody-epr19010-77-ab195241--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img169163.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC4 + 5 + 9 antibody [EPR19010-77] (AB195241)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | ChIP | Flow Cyt | WB | ICC/IF | IHC-P | |
|---|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Tested | Not recommended | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes - |
Species Rat | Dilution info - | Notes - |
ChIP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes - |
Species Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes - |
Species Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target data
Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. Deacetylates HSPA1A and HSPA1B at 'Lys-77' leading to their preferential binding to co-chaperone STUB1 (PubMed:27708256).
Additional Targets
HDAC5, HDAC9
Alternative names
KIAA0288, HDAC4, Histone deacetylase 4, HD4
Recommended products
Rabbit Recombinant Monoclonal HDAC4 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR19010-77
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
HDAC4 HDAC5 and HDAC9 are types of histone deacetylases abbreviated as HDACs. These enzymes remove acetyl groups from histone and non-histone proteins affecting gene expression by changing chromatin structure. HDAC4 has a molecular weight of about 140 kDa. HDAC4 and HDAC5 are highly expressed in the brain heart and skeletal muscle while HDAC9 is more abundant in the heart and skeletal muscle. They act as transcriptional repressors by interacting with various corepressor complexes.
- Biological function summary
Histone deacetylases 4 5 and 9 influence gene expression and cellular differentiation. These proteins often form part of larger corepressor complexes such as the N-CoR and SMRT complexes allowing them to modulate transcriptional activity. They play significant roles in muscle differentiation and neural development interacting with transcription factors like MEF2 to regulate various genes involved in these processes.
- Pathways
Histone deacetylases like HDAC4 5 and 9 are critical components of signaling pathways related to calcium/calmodulin-dependent calcineurin signal transduction. This pathway influences muscle development and function where they interact with MEF2 family transcription factors to control gene expression. Additionally they are involved in the regulation of the circadian rhythm through their role in the molecular clockwork partnering with proteins like CLOCK and BMAL1.
- Associated diseases and disorders
Alterations in HDAC4 5 and 9 levels or activity have connections to cardiovascular diseases and certain cancers. Dysfunctional HDAC activity can lead to hypertrophy in cardiac cells affecting heart function. Cancer progression often involves aberrant histone deacetylation with HDACs modulating oncogenic transcription factors. These HDACs closely interact with cancer-related proteins like MYC and p53 influencing tumor suppression or progression depending on cellular context.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-HDAC4 + 5 + 9 antibody [EPR19010-77] (ab195241), expandable thumbnail](https://content.abcam.com/products/images/hdac4-5-9-antibody-epr19010-77-ab195241--western-blot-img167589.jpg "Western blot - Anti-HDAC4 + 5 + 9 antibody [EPR19010-77] (ab195241)")
Western blot - Anti-HDAC4 + 5 + 9 antibody [EPR19010-77] (ab195241)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Reactivity with undetermined proteins which is probably the degradation fragment.
Exposure time: Lanes 1-2: 70 seconds;
Lane 3: 48 seconds.
All lanes: Western blot - Anti-HDAC4 + 5 + 9 antibody [EPR19010-77] (ab195241) at 1/5000 dilution
Lane 1: HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg
Lane 2: HUVEC (human umbilical vein endothelial cell) whole cell lysate at 20 µg
Lane 3: RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Predicted band size: 121 kDa
Observed band size: 140 kDa
![Western blot - Anti-HDAC4 + 5 + 9 antibody [EPR19010-77] (ab195241), expandable thumbnail](https://content.abcam.com/products/images/hdac4-5-9-antibody-epr19010-77-ab195241--western-blot-img167206.jpg "Western blot - Anti-HDAC4 + 5 + 9 antibody [EPR19010-77] (ab195241)")
Western blot - Anti-HDAC4 + 5 + 9 antibody [EPR19010-77] (ab195241)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Reactivity with undetermined proteins which is probably the degradation fragment.
Exposure time: 15 seconds.
All lanes: Western blot - Anti-HDAC4 + 5 + 9 antibody [EPR19010-77] (ab195241) at 1/5000 dilution
Lane 1: THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg
Lane 2: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg
Lane 3: PC-12 (rat adrenal gland pheochromocytoma ) whole cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Predicted band size: 121 kDa
Observed band size: 140 kDa
![Western blot - Anti-HDAC4 + 5 + 9 antibody [EPR19010-77] (ab195241), expandable thumbnail](https://content.abcam.com/products/images/hdac4-5-9-antibody-epr19010-77-ab195241--western-blot-img174153.jpg "Western blot - Anti-HDAC4 + 5 + 9 antibody [EPR19010-77] (ab195241)")
Western blot - Anti-HDAC4 + 5 + 9 antibody [EPR19010-77] (ab195241)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
This antibody reacts with HDAC5, HDAC4 and HDAC9, but not HDAC7.
Exposure time: Lane 1: 15 seconds;
Lanes 2-4: 3 minutes.
All lanes: Western blot - Anti-HDAC4 + 5 + 9 antibody [EPR19010-77] (ab195241) at 1/1000 dilution
Lane 1: E.coli extracts containing his-tagged human HDAC5 recombinant protein at 5 µL
Lane 2: E.coli extracts containing his-tagged human HDAC4 recombinant protein at 5 µL
Lane 3: E.coli extracts containing his-tagged human HDAC7 recombinant protein at 5 µL
Lane 4: E.coli extracts containing his-tagged human HDAC9 recombinant protein at 5 µL
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 121 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC4 + 5 + 9 antibody [EPR19010-77] (ab195241), expandable thumbnail](https://content.abcam.com/products/images/hdac4-5-9-antibody-epr19010-77-ab195241--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img182545.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC4 + 5 + 9 antibody [EPR19010-77] (ab195241)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC4 + 5 + 9 antibody [EPR19010-77] (ab195241)
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labelling HDAC4 + HDAC5 + HDAC9 with ab195241 at 1/100 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Nuclear and weak cytoplasmic staining on human cerebrum. The section was incubated with ab195241 for 15 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC4 + 5 + 9 antibody [EPR19010-77] (ab195241), expandable thumbnail](https://content.abcam.com/products/images/hdac4-5-9-antibody-epr19010-77-ab195241--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img169163.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC4 + 5 + 9 antibody [EPR19010-77] (ab195241)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC4 + 5 + 9 antibody [EPR19010-77] (ab195241)
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling HDAC4 + HDAC5 + HDAC9 with ab195241 at 1/100 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Nuclear and weak cytoplasmic staining on human breast carcinoma. The section was incubated with ab195241 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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