Anti-HDAC4 + 5 + 9 antibody [EPR19010-77] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC4 + 5 + 9 antibody [EPR19010-77] - BSA and Azide free (AB271355)

This data was developed using ab195241, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labelling HDAC4 + HDAC5 + HDAC9 with ab195241 at 1/100 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear and weak cytoplasmic staining on human cerebrum. The section was incubated with ab195241 for 15 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC4 + 5 + 9 antibody [EPR19010-77] - BSA and Azide free (AB271355)

This data was developed using ab195241, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling HDAC4 + HDAC5 + HDAC9 with ab195241 at 1/100 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear and weak cytoplasmic staining on human breast carcinoma. The section was incubated with ab195241 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• WB

Lab

Western blot - Anti-HDAC4 + 5 + 9 antibody [EPR19010-77] - BSA and Azide free (AB271355)

This data was developed using ab195241, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Reactivity with undetermined proteins which is probably the degradation fragment. Exposure time : Lanes 1-2 : 70 seconds; Lane 3 : 48 seconds.

All lanes:

Western blot - Anti-HDAC4 + 5 + 9 antibody [EPR19010-77] (<a href='/en-us/products/primary-antibodies/hdac4-5-9-antibody-epr19010-77-ab195241'>ab195241</a>) at 1/5000 dilution

Lane 1:

HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 2:

HUVEC (human umbilical vein endothelial cell) whole cell lysate at 20 µg

Lane 3:

RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 121 kDa

Observed band size: 140 kDa

false

• WB

Lab

Western blot - Anti-HDAC4 + 5 + 9 antibody [EPR19010-77] - BSA and Azide free (AB271355)

This data was developed using ab195241, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Reactivity with undetermined proteins which is probably the degradation fragment.

Exposure time : 15 seconds

All lanes:

Western blot - Anti-HDAC4 + 5 + 9 antibody [EPR19010-77] (<a href='/en-us/products/primary-antibodies/hdac4-5-9-antibody-epr19010-77-ab195241'>ab195241</a>) at 1/5000 dilution

Lane 1:

THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

Lane 2:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 3:

PC-12 (rat adrenal gland pheochromocytoma ) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 121 kDa

Observed band size: 140 kDa

false

• WB

Lab

Western blot - Anti-HDAC4 + 5 + 9 antibody [EPR19010-77] - BSA and Azide free (AB271355)

This data was developed using ab195241, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

This antibody reacts with HDAC5, HDAC4 and HDAC9, but not HDAC7.

Exposure time : Lane 1 : 15 seconds;

Lanes 2-4 : 3 minutes.

All lanes:

Western blot - Anti-HDAC4 + 5 + 9 antibody [EPR19010-77] (<a href='/en-us/products/primary-antibodies/hdac4-5-9-antibody-epr19010-77-ab195241'>ab195241</a>) at 1/1000 dilution

Lane 1:

E.coli extracts containing his-tagged human HDAC5 recombinant protein at 5 µL

Lane 2:

E.coli extracts containing his-tagged human HDAC4 recombinant protein at 5 µL

Lane 3:

E.coli extracts containing his-tagged human HDAC7 recombinant protein at 5 µL

Lane 4:

E.coli extracts containing his-tagged human HDAC9 recombinant protein at 5 µL

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 121 kDa

false