AB234084

Anti-HDAC4 antibody [7E2E6]

Name: Anti-HDAC4 antibody [7E2E6] (ab234084) | Abcam
Brand: Abcam Limited
SKU: AB234084
Price: 520 USD
Availability: InStock

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(3 Publications)

Mouse Monoclonal HDAC4 antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HDAC4 aa 450-600.

View Alternative Names

KIAA0288, HDAC4, Histone deacetylase 4, HD4

4 Images

Flow Cytometry - Anti-HDAC4 antibody [7E2E6] (AB234084)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-HDAC4 antibody [7E2E6] (AB234084)

Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinom) cells labeling HDAC4 using ab234084 at 1/200 dilution (green) compared to a negative control (red).

Immunocytochemistry/ Immunofluorescence - Anti-HDAC4 antibody [7E2E6] (AB234084)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HDAC4 antibody [7E2E6] (AB234084)

Immunofluorescence analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling HDAC4 using ab234084 at 1/200 dilution (green).

Blue : DRAQ5 fluorescent DNA dye. Red : Actin filaments labeled with AlexaFluor^®-555-Phalloidin.

Supplier Data

Western blot - Anti-HDAC4 antibody [7E2E6] (AB234084)

All lanes:

Western blot - Anti-HDAC4 antibody [7E2E6] (ab234084) at 1/100 dilution

Lane 1:

HEK-293 (human epithelial cell line from embryonic kidney) cell lysate.

Lane 2:

HDAC4 (aa 456-592)-hIgGFc-transfected HEK-293 cell lysate.

Predicted band size: 119 kDa

false

Supplier Data

Western blot - Anti-HDAC4 antibody [7E2E6] (AB234084)

All lanes:

Western blot - Anti-HDAC4 antibody [7E2E6] (ab234084) at 1/100 dilution

All lanes:

Human HDAC4 (aa 456-592) recombinant protein.

Predicted band size: 119 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

7E2E6

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

ICC/IF, WB, Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Human HDAC4 aa 450-600. The exact immunogen used to generate this antibody is proprietary information.

P56524

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/500", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200 - 1/1000", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from tissue culture supernatant.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HDAC4 also known as Histone Deacetylase 4 functions in the regulation of gene expression through chromatin remodeling. This protein removes acetyl groups from histone proteins causing chromatin compaction and transcriptional repression. HDAC4 has a molecular weight of approximately 140 kDa. The protein is found in various tissues including brain heart and skeletal muscle and plays essential roles in various cellular processes. Alternate names for HDAC4 include KIAA0288 and HD4.

Biological function summary

The role of HDAC4 extends beyond chromatin remodeling as it serves as a critical regulator in several cellular functions. As part of a complex HDAC4 interacts with other proteins to control cell cycle differentiation and apoptosis. It cooperates with transcriptional repressors like MEF2 and RUNX2 influencing various signaling pathways. The protein's activity impacts neuronal survival muscle development and cardiac hypertrophy through its regulatory mechanisms.

Pathways

HDAC4 integrates into significant signaling networks notably the MAPK and calcium-calmodulin signaling pathways. Within the MAPK pathway HDAC4 associates with proteins like MEF2 impacting cellular growth and differentiation processes. In the calcium-calmodulin pathway HDAC4 affects gene transcription via its interaction with the phosphatase calcineurin linking intracellular calcium levels with transcriptional responses.

HDAC4 has associations with neurological disorders and cancer. Mutations and dysregulation in HDAC4 are linked to neuronal disorders such as Huntington's disease. In cancer HDAC4 interacts with other oncogenic proteins such as p53 influencing tumor progression and resistance to therapy. Consequently HDAC4 presents as a potential target for therapeutic intervention in these disease contexts.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. Deacetylates HSPA1A and HSPA1B at 'Lys-77' leading to their preferential binding to co-chaperone STUB1 (PubMed : 27708256).

See full target information HDAC4

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Bioengineering (Basel, Switzerland) 11: PubMed39329689

2024

Mesenchymal Stem Cell-Conditioned Media-Loaded Microparticles Enhance Acute Patency in Silk-Based Vascular Grafts.

Applications

Unspecified application

Species

Unspecified reactive species

Katherine L Lorentz,Ande X Marini,Liza A Bruk,Prerak Gupta,Biman B Mandal,Morgan V DiLeo,Justin S Weinbaum,Steven R Little,David A Vorp

Oncology reports 45:169-179 PubMed33416147

2021

circHIPK3 promotes proliferation and migration and invasion via regulation of miR‑637/HDAC4 signaling in osteosarcoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Wen,Bin Li,Ming He,Songling Teng,Yuxiu Sun,Guangbin Wang

OncoTargets and therapy 13:1691-1704 PubMed32158233

2020

Long Noncoding RNA Acts as a microRNA-574 Sponge Thereby Enhancing the Aggressiveness of Gastric Cancer via HDAC4 Upregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaodong Wang,Xin Chen,Yueli Tian,Dongqiang Jiang,Ying Song

View all publications

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Product promise

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