Anti-HDAC4 antibody (ab12172) is a rabbit polyclonal antibody that is used to detect HDAC4 in Western Blot, IP, ICC/IF. Suitable for Human, Mouse, Rat samples.
- Over 30 publications
- Trusted since 2004
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- JCI insight 8:2023Comparative study in estrogen-depleted mice identifies skeletal and osteocyte transcriptomic responses to abaloparatide and teriparatide.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhengtao Lv,Jiaming Zhang,Shuang Liang,Chenhe Zhou,Dorothy Hu,Daniel J Brooks,Mary L Bouxsein,Beate Lanske,Paul Kostenuik,Francesca Gori,Roland BaronPubMed 37870958
- Molecular reproduction and development 90:849-8602023HDAC4 regulates the proliferation, migration, and invasion of trophoblasts in pre-eclampsia through the miR-134-5p/FOXM1 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanli Xu,Xiaodi Kang,Hongli Jiang,Huafang Liu,Wenjing WangPubMed 37769062
- American journal of translational research 14:8572-85872022Circular RNA FOXO3 regulates endometrial carcinoma progression through the microRNA-29a-3p/HDAC4 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLing Yang,Jinyin Xiao,Chengbin MaPubMed 36628205
- Neuropathology and applied neurobiology 49:e128612022Cytoplasmic HDAC4 recovers synaptic function in the 3×Tg mouse model of Alzheimer's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesClaudia Colussi,Giuseppe Aceto,Cristian Ripoli,Alessia Bertozzi,Domenica Donatella Li Puma,Elena Paccosi,Marcello D'Ascenzo,Claudio GrassiPubMed 36331820
- Frontiers in cell and developmental biology 10:8832662022The Critical Role of MMP13 in Regulating Tooth Development and Reactionary Dentinogenesis Repair Through the Wnt Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHenry F Duncan,Yoshifumi Kobayashi,Yukako Yamauchi,Angela Quispe-Salcedo,Zhi Chao Feng,Jia Huang,Nicola C Partridge,Teruyo Nakatani,Jeanine D'Armiento,Emi ShimizuPubMed 35531096
- Gerontology 68:1291-13102022KLF7 Alleviates Atherosclerotic Lesions and Inhibits Glucose Metabolic Reprogramming in Macrophages by Regulating HDAC4/miR-148b-3p/NCOR1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFangyuan Chen,Juanli Li,Tao Zheng,Tao Chen,Zuyi YuanPubMed 35439761
- Nature communications 13:18982022Virally programmed extracellular vesicles sensitize cancer cells to oncolytic virus and small molecule therapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarie-Eve Wedge,Victoria A Jennings,Mathieu J F Crupi,Joanna Poutou,Taylor Jamieson,Adrian Pelin,Giuseppe Pugliese,Christiano Tanese de Souza,Julia Petryk,Brian J Laight,Meaghan Boileau,Zaid Taha,Nouf Alluqmani,Hayley E McKay,Larissa Pikor,Sarwat Tahsin Khan,Taha Azad,Reza Rezaei,Bradley Austin,Xiaohong He,David Mansfield,Elaine Rose,Emily E F Brown,Natalie Crawford,Almohanad Alkayyal,Abera Surendran,Ragunath Singaravelu,Dominic G Roy,Gemma Migneco,Benjamin McSweeney,Mary Lynn Cottee,Egon J Jacobus,Brian A Keller,Takafumi N Yamaguchi,Paul C Boutros,Michele Geoffrion,Katey J Rayner,Avijit Chatterjee,Rebecca C Auer,Jean-Simon Diallo,Derrick Gibbings,Benjamin R tenOever,Alan Melcher,John C Bell,Carolina S IlkowPubMed 35393414
- Frontiers in physiology 13:8196192022Regulates the Proliferation of Neural Crest-Derived Osteoblasts During Murine Craniofacial Development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNayoung Ha,Jian Sun,Qian Bian,Dandan Wu,Xudong WangPubMed 35242053
- Cell death discovery 7:2402021Histone deacetylase HDAC4 participates in the pathological process of myocardial ischemia-reperfusion injury via MEKK1/JNK pathway by binding to miR-206.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQingman Li,Lijie Zhu,Fangqing Niu,Qingmin Li,Che Wang,Honghui Yang,Chuanyu GaoPubMed 34526481
- International journal of molecular sciences 22:2021Chondroprotective Effects of a Histone Deacetylase Inhibitor, Panobinostat, on Pain Behavior and Cartilage Degradation in Anterior Cruciate Ligament Transection-Induced Experimental Osteoarthritic Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhi-Hong Wen,Jhy-Shrian Huang,Yen-You Lin,Zhi-Kang Yao,Yu-Cheng Lai,Wu-Fu Chen,Hsin-Tzu Liu,Sung-Chun Lin,Yu-Chi Tsai,Tsung-Chang Tsai,Yen-Hsuan JeanPubMed 34298911
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 1% BSA, 0.0268% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human HDAC4 aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P56524
Reactivity data
Select an application| ICC/IF | IP | WB | |
|---|---|---|---|
| Human | Tested | Tested | Expected |
| Mouse | Expected | Expected | Tested |
| Rat | Expected | Expected | Tested |
| Chicken | Predicted | Predicted | Predicted |
| Rabbit | Predicted | Predicted | Predicted |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/250.00000 - 1/500.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Chicken | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes 10-20 μg of antibody immunoprecipitates HDAC4 from a RIPA extract of HeLa nuclei from 1 x 107 cells. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Chicken | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000.00000 - 1/2000.00000 | Notes Additional weak bands may be detected when blotting using different extract preparations. |
Species Rat | Dilution info 1/500.00000 - 1/1000.00000 | Notes Additional weak bands may be detected when blotting using different extract preparations. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Chicken | Dilution info - | Notes - |
Associated Products
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Target data
Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. Deacetylates HSPA1A and HSPA1B at 'Lys-77' leading to their preferential binding to co-chaperone STUB1 (PubMed:27708256).
Alternative names
KIAA0288, HDAC4, Histone deacetylase 4, HD4
Recommended products
Anti-HDAC4 antibody (ab12172) is a rabbit polyclonal antibody that is used to detect HDAC4 in Western Blot, IP, ICC/IF. Suitable for Human, Mouse, Rat samples.
- Over 30 publications
- Trusted since 2004
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 1% BSA, 0.0268% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human HDAC4 aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P56524
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
The antibody is affinity-purified using the immunizing peptide immobilized on agarose.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-HDAC4 antibody (ab12172) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.
What is the molecular weight of HDAC4?
Anti-HDAC4 (ab12172) specifically detects a band for HDAC4 (UniProt: P56524) at a molecular weight of 119kDa.
Trusted by the scientific community
Anti-HDAC4 (ab12172) was first used in a scientific publication in 2004 and has been cited over 30 times in peer-reviewed journals.
Reviewed by scientists
Anti-HDAC4 (ab12172) has over 5 independent reviews from customers.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
HDAC4 also known as Histone Deacetylase 4 functions in the regulation of gene expression through chromatin remodeling. This protein removes acetyl groups from histone proteins causing chromatin compaction and transcriptional repression. HDAC4 has a molecular weight of approximately 140 kDa. The protein is found in various tissues including brain heart and skeletal muscle and plays essential roles in various cellular processes. Alternate names for HDAC4 include KIAA0288 and HD4.
- Biological function summary
The role of HDAC4 extends beyond chromatin remodeling as it serves as a critical regulator in several cellular functions. As part of a complex HDAC4 interacts with other proteins to control cell cycle differentiation and apoptosis. It cooperates with transcriptional repressors like MEF2 and RUNX2 influencing various signaling pathways. The protein's activity impacts neuronal survival muscle development and cardiac hypertrophy through its regulatory mechanisms.
- Pathways
HDAC4 integrates into significant signaling networks notably the MAPK and calcium-calmodulin signaling pathways. Within the MAPK pathway HDAC4 associates with proteins like MEF2 impacting cellular growth and differentiation processes. In the calcium-calmodulin pathway HDAC4 affects gene transcription via its interaction with the phosphatase calcineurin linking intracellular calcium levels with transcriptional responses.
- Associated diseases and disorders
HDAC4 has associations with neurological disorders and cancer. Mutations and dysregulation in HDAC4 are linked to neuronal disorders such as Huntington's disease. In cancer HDAC4 interacts with other oncogenic proteins such as p53 influencing tumor progression and resistance to therapy. Consequently HDAC4 presents as a potential target for therapeutic intervention in these disease contexts.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Immunoprecipitation - Anti-HDAC4 antibody (ab12172)
Anti-HDAC4 antibody, ab12172, was used to immunoprecipitate HDAC4 from HeLa whole cell extract.Lane 1: 10 μg of ab12172Lane 2: 5 μg of ab12172Negative control: No cell extractPositive control: HeLa whole cell extract
All lanes: Immunoprecipitation - Anti-HDAC4 antibody (ab12172)
Predicted band size: 119 kDa
Immunocytochemistry/ Immunofluorescence - Anti-HDAC4 antibody (ab12172)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Hela tissue labelling HDAC4 with ab12172 at 1/250 dilution.
Western blot - Anti-HDAC4 antibody (ab12172)
Lane 1: Western blot - Anti-HDAC4 antibody (ab12172) at 1/500 dilution
Lane 2: Western blot - Anti-HDAC4 antibody (ab12172) at 1/1000 dilution
Lanes 1 - 2: Rat brain whole extract
Lane 3: Negative Control
Predicted band size: 119 kDa
Western blot - Anti-HDAC4 antibody (ab12172)
Lane 1: Western blot - Anti-HDAC4 antibody (ab12172) at 1/1000 dilution
Lane 2: Western blot - Anti-HDAC4 antibody (ab12172) at 1/2000 dilution
Lanes 1 - 2: NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell extract
Lane 3: Negative Control
Predicted band size: 119 kDa
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