AB12172

Anti-HDAC4 antibody

Name: Anti-HDAC4 antibody (ab12172) | Abcam
Brand: Abcam Limited
SKU: AB12172
Price: 450 USD
Availability: InStock
Rating: 4 (8 reviews)

(8 Reviews)

(43 Publications)

Anti-HDAC4 antibody (ab12172) is a rabbit polyclonal antibody detecting HDAC4 in Western Blot, IP, ICC/IF. Suitable for Human, Mouse, Rat.

- Over 30 publications
- Trusted since 2004

View Alternative Names

KIAA0288, HDAC4, Histone deacetylase 4, HD4

4 Images

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Immunoprecipitation - Anti-HDAC4 antibody (AB12172)

Anti-HDAC4 antibody, ab12172, was used to immunoprecipitate HDAC4 from HeLa whole cell extract.

Lane 1 : 10 μg of ab12172
Lane 2 : 5 μg of ab12172

Negative control : No cell extract

Positive control : HeLa whole cell extract

All lanes:

Immunoprecipitation - Anti-HDAC4 antibody (ab12172)

Predicted band size: 119 kDa

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Immunocytochemistry/ Immunofluorescence - Anti-HDAC4 antibody (AB12172)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-HDAC4 antibody (AB12172)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Hela tissue labelling HDAC4 with ab12172 at 1/250 dilution.

Supplier Data

Western blot - Anti-HDAC4 antibody (AB12172)

Lane 1:

Western blot - Anti-HDAC4 antibody (ab12172) at 1/1000 dilution

Lane 2:

Western blot - Anti-HDAC4 antibody (ab12172) at 1/2000 dilution

Lanes 1 - 2:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell extract

Lane 3:

Negative Control

Predicted band size: 119 kDa

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Supplier Data

Western blot - Anti-HDAC4 antibody (AB12172)

Lane 1:

Western blot - Anti-HDAC4 antibody (ab12172) at 1/500 dilution

Lane 2:

Western blot - Anti-HDAC4 antibody (ab12172) at 1/1000 dilution

Lanes 1 - 2:

Rat brain whole extract

Lane 3:

Negative Control

Predicted band size: 119 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Rat, Mouse

Applications

IP, WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human HDAC4 aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P56524

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "10-20 μg of antibody immunoprecipitates HDAC4 from a RIPA extract of HeLa nuclei from 1 x 107 cells.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "Additional weak bands may be detected when blotting using different extract preparations." }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "Additional weak bands may be detected when blotting using different extract preparations." }, "Chicken": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-HDAC4 antibody (ab12172) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of HDAC4?
Anti-HDAC4 (ab12172) specifically detects a band for HDAC4 (UniProt: P56524) at a molecular weight of 119kDa.

Trusted by the scientific community
Anti-HDAC4 (ab12172) was first used in a scientific publication in 2004 and has been cited over 30 times in peer-reviewed journals.

Reviewed by scientists
Anti-HDAC4 (ab12172) has over 5 independent reviews from customers.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

The antibody is affinity-purified using the immunizing peptide immobilized on agarose.

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HDAC4 also known as Histone Deacetylase 4 functions in the regulation of gene expression through chromatin remodeling. This protein removes acetyl groups from histone proteins causing chromatin compaction and transcriptional repression. HDAC4 has a molecular weight of approximately 140 kDa. The protein is found in various tissues including brain heart and skeletal muscle and plays essential roles in various cellular processes. Alternate names for HDAC4 include KIAA0288 and HD4.

Biological function summary

The role of HDAC4 extends beyond chromatin remodeling as it serves as a critical regulator in several cellular functions. As part of a complex HDAC4 interacts with other proteins to control cell cycle differentiation and apoptosis. It cooperates with transcriptional repressors like MEF2 and RUNX2 influencing various signaling pathways. The protein's activity impacts neuronal survival muscle development and cardiac hypertrophy through its regulatory mechanisms.

Pathways

HDAC4 integrates into significant signaling networks notably the MAPK and calcium-calmodulin signaling pathways. Within the MAPK pathway HDAC4 associates with proteins like MEF2 impacting cellular growth and differentiation processes. In the calcium-calmodulin pathway HDAC4 affects gene transcription via its interaction with the phosphatase calcineurin linking intracellular calcium levels with transcriptional responses.

HDAC4 has associations with neurological disorders and cancer. Mutations and dysregulation in HDAC4 are linked to neuronal disorders such as Huntington's disease. In cancer HDAC4 interacts with other oncogenic proteins such as p53 influencing tumor progression and resistance to therapy. Consequently HDAC4 presents as a potential target for therapeutic intervention in these disease contexts.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. Deacetylates HSPA1A and HSPA1B at 'Lys-77' leading to their preferential binding to co-chaperone STUB1 (PubMed : 27708256).

See full target information HDAC4

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Publications (43)

Recent publications for all applications. Explore the full list and refine your search

Cell biochemistry and function 43:e70064 PubMed40103178

2025

HDAC4-AS1/CTCF Transcriptionally Represses HDAC4 Under Stress, Whereas HDAC4 Inhibits Stress-Induced Syncytiotrophoblast Cellular Pyroptosis by Deacetylating NLRP3 and GSDMD.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Du,Qinghong Ji,Lihua Dong,Lanlan Wang,Gang Xin

Pharmaceuticals (Basel, Switzerland) 17: PubMed39598328

2024

Magnolol Inhibits High Fructose-Induced Podocyte Inflammation via Downregulation of TKFC/Sp1/HDAC4/Notch1 Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Ziang Zhou,Yumeng Wang,Yu Xing,Shuman Pan,Wanru Wang,Jie Yang,Wenyuan Wu,Jie Zhou,Luyi Huang,Qiongdan Liang,Dongmei Zhang,Lingdong Kong

Pain reports 9:e1209 PubMed39512583

2024

Upregulated spinal histone deacetylases induce nociceptive sensitization by inhibiting the GABA system in chronic constriction injury-induced neuropathy in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi-Hong Wen,Nan-Fu Chen,Hao-Jung Cheng,Hsiao-Mei Kuo,Pei-Yu Chen,Chien-Wei Feng,Zhi-Kang Yao,Wu-Fu Chen,Chun-Sung Sung

Cell communication and signaling : CCS 22:488 PubMed39394127

2024

Nesfatin-1 enhances vascular smooth muscle calcification through facilitating BMP-2 osteogenic signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Xue-Xue Zhu,Xin-Yu Meng,Guo Chen,Jia-Bao Su,Xiao Fu,An-Jing Xu,Yao Liu,Xiao-Hui Hou,Hong-Bo Qiu,Qing-Yi Sun,Jin-Yi Hu,Zhuo-Lin Lv,Hai-Jian Sun,Hai-Bin Jiang,Zhi-Jun Han,Jian Zhu,Qing-Bo Lu

Stem cell research & therapy 15:275 PubMed39227892

2024

Nucleoporin 153 deficiency in adult neural stem cells defines a pathological protein-network signature and defective neurogenesis in a mouse model of AD.

Applications

Unspecified application

Species

Unspecified reactive species

Claudia Colussi,Alessia Bertozzi,Lucia Leone,Marco Rinaudo,Raimondo Sollazzo,Federica Conte,Elena Paccosi,Luca Nardella,Giuseppe Aceto,Domenica Donatella Li Puma,Cristian Ripoli,Maria Gabriella Vita,Camillo Marra,Marcello D'Ascenzo,Claudio Grassi

Redox biology 76:103317 PubMed39180980

2024

AQP1 differentially orchestrates endothelial cell senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Khatereh Shabanian,Taraneh Shabanian,Gergely Karsai,Luca Pontiggia,Francesco Paneni,Frank Ruschitzka,Jürg H Beer,Seyed Soheil Saeedi Saravi

Archives of medical science : AMS 20:847-862 PubMed39050159

2024

LINC00847 drives pancreatic cancer progression by targeting the miR-455-3p/HDAC4 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Shunxin Hao,Zhi Yao,Yifeng Liu

Cartilage :19476035241264012 PubMed39057748

2024

Effects of Etanercept on Experimental Osteoarthritis in Rats: Role of Histone Deacetylases.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi-Hong Wen,Chi-Chieh Tang,Yen-You Lin,Zhi-Kang Yao,Shih-Peng Hsieh, Gar-Hwa-Lai,Wu-Fu Chen,Yen-Hsuan Jean

iScience 27:108689 PubMed38226157

2024

High glucose impairs cognitive function through inducing mitochondrial calcium overload in Treg cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ya Hui,Liuyu Kuang,Yuanmei Zhong,Yunyun Tang,Zhiqiang Xu,Tianpeng Zheng

Aging 16:685-700 PubMed38217540

2024

ZIP4 upregulation aggravates nucleus pulposus cell degradation by promoting inflammation and oxidative stress by mediating the HDAC4-FoxO3a axis.

Applications

Unspecified application

Species

Unspecified reactive species

Mingkui Shen,Kuankuan Li,Lulu Wang,Li Feng,Xinyu Zhang,Haoping Zhang,Honggang Zhou,Guoxian Pei

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HDAC4 antibody

Immunoprecipitation - Anti-HDAC4 antibody (AB12172)

Immunocytochemistry/ Immunofluorescence - Anti-HDAC4 antibody (AB12172)

Western blot - Anti-HDAC4 antibody (AB12172)

Western blot - Anti-HDAC4 antibody (AB12172)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (43)

Product promise