AB79521

Anti-HDAC4 antibody

Name: Anti-HDAC4 antibody (ab79521) | Abcam
Brand: Abcam Limited
SKU: AB79521
Price: 620 USD
Availability: InStock

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(10 Publications)

Rabbit Polyclonal HDAC4 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human HDAC4 aa 150-250.

View Alternative Names

KIAA0288, HDAC4, Histone deacetylase 4, HD4

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC4 antibody (AB79521)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC4 antibody (AB79521)

ab79521 at 5μg/ml staining human HDAC4 by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded lung, respiratory epithelium sections).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human HDAC4 aa 150-250. The exact immunogen used to generate this antibody is proprietary information.

P56524

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HDAC4 also known as Histone Deacetylase 4 functions in the regulation of gene expression through chromatin remodeling. This protein removes acetyl groups from histone proteins causing chromatin compaction and transcriptional repression. HDAC4 has a molecular weight of approximately 140 kDa. The protein is found in various tissues including brain heart and skeletal muscle and plays essential roles in various cellular processes. Alternate names for HDAC4 include KIAA0288 and HD4.

Biological function summary

The role of HDAC4 extends beyond chromatin remodeling as it serves as a critical regulator in several cellular functions. As part of a complex HDAC4 interacts with other proteins to control cell cycle differentiation and apoptosis. It cooperates with transcriptional repressors like MEF2 and RUNX2 influencing various signaling pathways. The protein's activity impacts neuronal survival muscle development and cardiac hypertrophy through its regulatory mechanisms.

Pathways

HDAC4 integrates into significant signaling networks notably the MAPK and calcium-calmodulin signaling pathways. Within the MAPK pathway HDAC4 associates with proteins like MEF2 impacting cellular growth and differentiation processes. In the calcium-calmodulin pathway HDAC4 affects gene transcription via its interaction with the phosphatase calcineurin linking intracellular calcium levels with transcriptional responses.

HDAC4 has associations with neurological disorders and cancer. Mutations and dysregulation in HDAC4 are linked to neuronal disorders such as Huntington's disease. In cancer HDAC4 interacts with other oncogenic proteins such as p53 influencing tumor progression and resistance to therapy. Consequently HDAC4 presents as a potential target for therapeutic intervention in these disease contexts.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. Deacetylates HSPA1A and HSPA1B at 'Lys-77' leading to their preferential binding to co-chaperone STUB1 (PubMed : 27708256).

See full target information HDAC4

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Journal of innate immunity 14:366-379 PubMed35780770

2022

SP1 Promotes HDAC4 Expression and Inhibits HMGB1 Expression to Reduce Intestinal Barrier Dysfunction, Oxidative Stress, and Inflammatory Response after Sepsis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen-Mi Liu,Xi Wang,Chen-Xi Li,Xue-Yan Liu,Xiao-Jing Guo,Yang Li,You-Lian Chen,Hong-Xing Ye,Huai-Sheng Chen

International journal of molecular sciences 22: PubMed34445342

2021

Evaluation of Class IIa Histone Deacetylases Expression and In Vivo Epigenetic Imaging in a Transgenic Mouse Model of Alzheimer's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-An Chen,Cheng-Hsiu Lu,Chien-Chih Ke,Sain-Jhih Chiu,Chi-Wei Chang,Bang-Hung Yang,Juri G Gelovani,Ren-Shyan Liu

Journal of cellular physiology 236:7001-7013 PubMed33724469

2021

PTHrP promotes development of mouse preimplantation embryos through the AKT/cyclin D1 pathway and nuclear translocation of HDAC4.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan-Yuan Li,Lei Guo,Hui Li,Wen-Long Lei,Li-Hua Fan,Ying-Chun Ouyang,Yi Hou,Zhen-Bo Wang,Qing-Yuan Sun,Sheng-Sheng Lu,Zhiming Han

Clinical epigenetics 13:53 PubMed33691773

2021

Inhibition of HDAC4 by GSK3β leads to downregulation of KLF5 and ASK1 and prevents the progression of intravertebral disc degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Xiao,Dongping Gong,Loufeng Liang,Anwei Liang,Huaxin Liang,Xiayi Xu,Hongli Teng

Nature communications 10:4131 PubMed31511510

2019

TGFβ blocks IFNα/β release and tumor rejection in spontaneous mammary tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Marion V Guerin,Fabienne Regnier,Vincent Feuillet,Lene Vimeux,Julia M Weiss,Georges Bismuth,Gregoire Altan-Bonnet,Thomas Guilbert,Maxime Thoreau,Veronica Finisguerra,Emmanuel Donnadieu,Alain Trautmann,Nadège Bercovici

Brain, behavior, and immunity 61:340-352 PubMed27993690

2016

A seasonal switch in histone deacetylase gene expression in the hypothalamus and their capacity to modulate nuclear signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Patrick N Stoney,Diana Rodrigues,Gisela Helfer,Thabat Khatib,Anna Ashton,Elizabeth A Hay,Robert Starr,Dagmara Kociszewska,Peter Morgan,Peter McCaffery

Scientific reports 4:5282 PubMed24923522

2014

Epigenetic modification of the leptin promoter in diet-induced obese mice and the effects of N-3 polyunsaturated fatty acids.

Applications

ChIP

Species

Mouse

Wenwen Shen,Cui Wang,Lulu Xia,Chaonan Fan,Hua Dong,Richard J Deckelbaum,Kemin Qi

The Journal of clinical investigation 123:4706-13 PubMed24216484

2013

Eating disorder predisposition is associated with ESRRA and HDAC4 mutations.

Applications

Unspecified application

Species

Unspecified reactive species

Huxing Cui,Jarrette Moore,Sunbola S Ashimi,Brittany L Mason,Jordan N Drawbridge,Shizhong Han,Benjamin Hing,Abigail Matthews,Carrie J McAdams,Benjamin W Darbro,Andrew A Pieper,David A Waller,Chao Xing,Michael Lutter

PloS one 8:e58473 PubMed23469282

2013

Monoaminergic and neuropeptidergic neurons have distinct expression profiles of histone deacetylases.

Applications

ICC/IF

Species

Mouse

Kenkichi Takase,Satoko Oda,Masaru Kuroda,Hiromasa Funato

PloS one 6:e18950 PubMed21526203

2011

Fasting and high-fat diet alter histone deacetylase expression in the medial hypothalamus.

Applications

IHC-FoFr

Species

Mouse

Hiromasa Funato,Satoko Oda,Junko Yokofujita,Hiroaki Igarashi,Masaru Kuroda

View all publications

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