Skip to main content

Rabbit Recombinant Monoclonal HDAC4 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 4 publications.

Be the first to review this product! Submit a review

Images

Western blot - Anti-HDAC4 antibody [EPR22937-157] (AB235583), expandable thumbnail
  • Western blot - Anti-HDAC4 antibody [EPR22937-157] (AB235583), expandable thumbnail
  • Western blot - Anti-HDAC4 antibody [EPR22937-157] (AB235583), expandable thumbnail
  • Western blot - Anti-HDAC4 antibody [EPR22937-157] (AB235583), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBIHC-PICC/IF
Human
Not recommended
Not recommended
Tested
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Tested
Not recommended
Not recommended

Not recommended
Not recommended

Species

Human, Mouse

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info
1/1000
Notes

-

Species

Human

Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species

Mouse, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse

Dilution info

-

Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. Deacetylates HSPA1A and HSPA1B at 'Lys-77' leading to their preferential binding to co-chaperone STUB1 (PubMed:27708256).

Alternative names

KIAA0288, HDAC4, Histone deacetylase 4, HD4

Recommended products

Rabbit Recombinant Monoclonal HDAC4 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 4 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR22937-157

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

HDAC4 also known as Histone Deacetylase 4 functions in the regulation of gene expression through chromatin remodeling. This protein removes acetyl groups from histone proteins causing chromatin compaction and transcriptional repression. HDAC4 has a molecular weight of approximately 140 kDa. The protein is found in various tissues including brain heart and skeletal muscle and plays essential roles in various cellular processes. Alternate names for HDAC4 include KIAA0288 and HD4.

Biological function summary

The role of HDAC4 extends beyond chromatin remodeling as it serves as a critical regulator in several cellular functions. As part of a complex HDAC4 interacts with other proteins to control cell cycle differentiation and apoptosis. It cooperates with transcriptional repressors like MEF2 and RUNX2 influencing various signaling pathways. The protein's activity impacts neuronal survival muscle development and cardiac hypertrophy through its regulatory mechanisms.

Pathways

HDAC4 integrates into significant signaling networks notably the MAPK and calcium-calmodulin signaling pathways. Within the MAPK pathway HDAC4 associates with proteins like MEF2 impacting cellular growth and differentiation processes. In the calcium-calmodulin pathway HDAC4 affects gene transcription via its interaction with the phosphatase calcineurin linking intracellular calcium levels with transcriptional responses.

Associated diseases and disorders

HDAC4 has associations with neurological disorders and cancer. Mutations and dysregulation in HDAC4 are linked to neuronal disorders such as Huntington's disease. In cancer HDAC4 interacts with other oncogenic proteins such as p53 influencing tumor progression and resistance to therapy. Consequently HDAC4 presents as a potential target for therapeutic intervention in these disease contexts.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-HDAC4 antibody [EPR22937-157] (ab235583), expandable thumbnail

    Western blot - Anti-HDAC4 antibody [EPR22937-157] (ab235583)

    ab235583 was shown to specifically react with HDAC4 in wild-type HAP1 cells as signal was lost in HDAC4 knockout cells. Wild-type and HDAC4 knockout samples were subjected to SDS-PAGE.

    ab235583 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.

    Exposure time: 3 minutes.

    Blocking and Diluting Buffer and concentration: 5% NFDM/TBST.

    All lanes: Western blot - Anti-HDAC4 antibody [EPR22937-157] (ab235583) at 1/1000 dilution

    Lane 1: Wild-type HAP1 whole cell lysate at 20 µg

    Lane 2: HDAC4 knockout HAP1 whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 119 kDa

    Observed band size: 140 kDa

  • Western blot - Anti-HDAC4 antibody [EPR22937-157] (ab235583), expandable thumbnail

    Western blot - Anti-HDAC4 antibody [EPR22937-157] (ab235583)

    Exposure time: 3 minutes.

    Blocking and Diluting Buffer and concentration: 5% NFDM/TBST.

    All lanes: Western blot - Anti-HDAC4 antibody [EPR22937-157] (ab235583) at 1/1000 dilution

    Lane 1: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 20 µg

    Lane 2: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 119 kDa

    Observed band size: 140 kDa

  • Western blot - Anti-HDAC4 antibody [EPR22937-157] (ab235583), expandable thumbnail

    Western blot - Anti-HDAC4 antibody [EPR22937-157] (ab235583)

    Lanes 3-4 in this blot were developed using a higher sensitivity ECL substrate.

    Exposure time: Lanes 1-2: 81 seconds Lane 3: 48 seconds Lane 4: 15 seconds.

    Blocking and Diluting Buffer and concentration: 5% NFDM/TBST.

    All lanes: Western blot - Anti-HDAC4 antibody [EPR22937-157] (ab235583) at 1/1000 dilution

    Lane 1: HEK-293T (human embryonic kidney epithelial cell), whole cell lysate at 20 µg

    Lane 2: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 3: A549 (human lung carcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 4: Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 119 kDa

    Observed band size: 140 kDa

  • Western blot - Anti-HDAC4 antibody [EPR22937-157] (ab235583), expandable thumbnail

    Western blot - Anti-HDAC4 antibody [EPR22937-157] (ab235583)

    Western blot: Anti-HDAC4 antibody [EPR22937-157] (ab235583) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab235583 was shown to bind specifically to HDAC4. A band was observed at 140 kDa in wild-type A549 cell lysates with no signal observed at this size in HDAC4 knockout cell line. To generate this image, wild-type and HDAC4 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    All lanes: Western blot - Anti-HDAC4 antibody [EPR22937-157] (ab235583) at 1/1000 dilution

    Lane 1: Wild-type A549 cell lysate at 20 µg

    Lane 2: HDAC4 knockout A549 cell lysate at 20 µg

    Secondary

    All lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size: 140 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com