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AB12171

Anti-HDAC4 antibody [HDAC-144]

3

(4 Reviews)

|

(13 Publications)

Mouse Monoclonal HDAC4 antibody. Suitable for ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 13 publications. Immunogen corresponding to Synthetic Peptide within Human HDAC4 aa 1-50.

View Alternative Names

KIAA0288, HDAC4, Histone deacetylase 4, HD4

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

HDAC-144

Isotype

IgG2a

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF

applications

Immunogen

Synthetic Peptide within Human HDAC4 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P56524

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Alternative Products

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Primary Antibodies

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Primary Antibodies

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Primary Antibodies

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Primary Antibodies

AB256827

Anti-HDAC4 antibody [EPR22937-157] - BSA and Azide free

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Primary Antibodies

AB235583

Anti-HDAC4 antibody [EPR22937-157]

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Western blot - Anti-HDAC4 antibody [EPR22937-157] (AB235583)

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Primary Antibodies

AB12172

Anti-HDAC4 antibody

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Complementary Products

Assay Kits

AB300311

Human HDAC4 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human HDAC4 ELISA Kit (AB300311)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

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Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

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Primary Antibodies

AB195241

Anti-HDAC4 + 5 + 9 antibody [EPR19010-77]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC4 + 5 + 9 antibody [EPR19010-77] (AB195241)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Chicken": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

This product was changed from ascites to tissue culture supernatant on 23/05/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.4 Preservative: 0.0975% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HDAC4 also known as Histone Deacetylase 4 functions in the regulation of gene expression through chromatin remodeling. This protein removes acetyl groups from histone proteins causing chromatin compaction and transcriptional repression. HDAC4 has a molecular weight of approximately 140 kDa. The protein is found in various tissues including brain heart and skeletal muscle and plays essential roles in various cellular processes. Alternate names for HDAC4 include KIAA0288 and HD4.
Biological function summary

The role of HDAC4 extends beyond chromatin remodeling as it serves as a critical regulator in several cellular functions. As part of a complex HDAC4 interacts with other proteins to control cell cycle differentiation and apoptosis. It cooperates with transcriptional repressors like MEF2 and RUNX2 influencing various signaling pathways. The protein's activity impacts neuronal survival muscle development and cardiac hypertrophy through its regulatory mechanisms.

Pathways

HDAC4 integrates into significant signaling networks notably the MAPK and calcium-calmodulin signaling pathways. Within the MAPK pathway HDAC4 associates with proteins like MEF2 impacting cellular growth and differentiation processes. In the calcium-calmodulin pathway HDAC4 affects gene transcription via its interaction with the phosphatase calcineurin linking intracellular calcium levels with transcriptional responses.

HDAC4 has associations with neurological disorders and cancer. Mutations and dysregulation in HDAC4 are linked to neuronal disorders such as Huntington's disease. In cancer HDAC4 interacts with other oncogenic proteins such as p53 influencing tumor progression and resistance to therapy. Consequently HDAC4 presents as a potential target for therapeutic intervention in these disease contexts.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. Deacetylates HSPA1A and HSPA1B at 'Lys-77' leading to their preferential binding to co-chaperone STUB1 (PubMed : 27708256).
See full target information HDAC4
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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:566 PubMed40186004

2025

Glucocorticoids regulate the expression of Srsf1 through Hdac4/Foxc1 axis to induce apoptosis of osteoblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Luo,Tao Wang,Zhihong Xie,Fanchao Li,Chengyou Yang,Wentao Dong,Jianhua Wu,Qiang Wang,Fengyang Xu,Jiong Liu,Fei Zhang,Wuxun Peng

Journal of immunology research 2023:8571649 PubMed36644540

2023

FTY720 Attenuates LPS-Induced Inflammatory Bone Loss by Inhibiting Osteoclastogenesis via the NF-B and HDAC4/ATF Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Chongwei Chen,Sujing Zong,Zhenyu Wang,Ruijia Yang,Yanjing Guo,Yunfei Wang,Xinping Chen,Yue Li,Shaowei Wang

Journal of medicinal chemistry 65:12445-12459 PubMed36098485

2022

Developing HDAC4-Selective Protein Degraders To Investigate the Role of HDAC4 in Huntington's Disease Pathology.

Applications

Unspecified application

Species

Unspecified reactive species

Natsuko Macabuag,William Esmieu,Perla Breccia,Rebecca Jarvis,Wesley Blackaby,Ovadia Lazari,Liudvikas Urbonas,Maria Eznarriaga,Rachel Williams,Annelieke Strijbosch,Rhea Van de Bospoort,Kim Matthews,Cole Clissold,Tammy Ladduwahetty,Huw Vater,Patrick Heaphy,Douglas G Stafford,Hong-Jun Wang,John E Mangette,George McAllister,Vahri Beaumont,Thomas F Vogt,Hilary A Wilkinson,Elizabeth M Doherty,Celia Dominguez

Cell death discovery 7:240 PubMed34526481

2021

Histone deacetylase HDAC4 participates in the pathological process of myocardial ischemia-reperfusion injury via MEKK1/JNK pathway by binding to miR-206.

Applications

Unspecified application

Species

Unspecified reactive species

Qingman Li,Lijie Zhu,Fangqing Niu,Qingmin Li,Che Wang,Honghui Yang,Chuanyu Gao

Molecular carcinogenesis 59:1292-1301 PubMed32924161

2020

Protective role of histone deacetylase 4 from ultraviolet radiation-induced DNA lesions.

Applications

Unspecified application

Species

Unspecified reactive species

Shanshan Li,Mi Zhou,Kan Ze,Xiaoying Sun,Chunming Zhao,Zhouru Li,Haiyang Lu,Ying Jiao,Tianyang Wang,Su Li,Liang Hua,Hongxing Cai,Xin Li

Investigative ophthalmology & visual science 61:17 PubMed32915982

2020

Histone Deacetylases Regulation by δ-Opioids in Human Optic Nerve Head Astrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Syed A H Zaidi,Nakul Thakore,Sudha Singh,Wendy Guzman,Shikhar Mehrotra,Vamsi Gangaraju,Shahid Husain

Nature genetics 51:1580-1587 PubMed31659325

2019

HDAC9 is implicated in atherosclerotic aortic calcification and affects vascular smooth muscle cell phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Rajeev Malhotra,Andreas C Mauer,Christian L Lino Cardenas,Xiuqing Guo,Jie Yao,Xiaoling Zhang,Florian Wunderer,Albert V Smith,Quenna Wong,Sonali Pechlivanis,Shih-Jen Hwang,Judy Wang,Lingyi Lu,Christopher J Nicholson,Georgia Shelton,Mary D Buswell,Hanna J Barnes,Haakon H Sigurslid,Charles Slocum,Caitlin O' Rourke,David K Rhee,Aranya Bagchi,Sagar U Nigwekar,Emmanuel S Buys,Catherine Y Campbell,Tamara Harris,Matthew Budoff,Michael H Criqui,Jerome I Rotter,Andrew D Johnson,Ci Song,Nora Franceschini,Stephanie Debette,Udo Hoffmann,Hagen Kälsch,Markus M Nöthen,Sigurdur Sigurdsson,Barry I Freedman,Donald W Bowden,Karl-Heinz Jöckel,Susanne Moebus,Raimund Erbel,Mary F Feitosa,Vilmundur Gudnason,George Thanassoulis,Warren M Zapol,Mark E Lindsay,Donald B Bloch,Wendy S Post,Christopher J O'Donnell

Cell stem cell 24:93-106.e6 PubMed30503143

2018

Single-Cell Sequencing of iPSC-Dopamine Neurons Reconstructs Disease Progression and Identifies HDAC4 as a Regulator of Parkinson Cell Phenotypes.

Applications

Unspecified application

Species

Unspecified reactive species

Charmaine Lang,Kieran R Campbell,Brent J Ryan,Phillippa Carling,Moustafa Attar,Jane Vowles,Olga V Perestenko,Rory Bowden,Fahd Baig,Meike Kasten,Michele T Hu,Sally A Cowley,Caleb Webber,Richard Wade-Martins

Endocrinology 158:2391-2405 PubMed28368536

2017

Transcription Factor CREM Mediates High Glucose Response in Cardiomyocytes and in a Male Mouse Model of Prolonged Hyperglycemia.

Applications

Unspecified application

Species

Unspecified reactive species

Saviana A Barbati,Claudia Colussi,Lorenza Bacci,Aurora Aiello,Agnese Re,Egidio Stigliano,Andrea M Isidori,Claudio Grassi,Alfredo Pontecorvi,Antonella Farsetti,Carlo Gaetano,Simona Nanni

Endocrinology 152:2760-7 PubMed21586557

2011

Histone deacetylation during brain development is essential for permanent masculinization of sexual behavior.

Applications

ChIP, WB

Species

Rat, Rat

Ken Ichi Matsuda,Hiroko Mori,Bridget M Nugent,Donald W Pfaff,Margaret M McCarthy,Mitsuhiro Kawata
View all publications
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Product promise

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