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Rabbit Recombinant Monoclonal HDAC5 phospho S259 antibody. Carrier free. Suitable for Dot, IP, WB and reacts with Synthetic peptide - Human, Synthetic peptide - Mouse, Synthetic peptide - Rat, Human, Mouse, Rat samples.

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Images

Immunoprecipitation - Anti-HDAC4 (phospho S246) + HDAC5 (phospho S259) + HDAC7 antibody [EPR22997-46] - BSA and Azide free (AB264560), expandable thumbnail
  • Dot Blot - Anti-HDAC4 (phospho S246) + HDAC5 (phospho S259) + HDAC7 antibody [EPR22997-46] - BSA and Azide free (AB264560), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
DotIPFlow CytWBIHC-PICC/IF
Human
Expected
Tested
Not recommended
Tested
Not recommended
Not recommended
Mouse
Expected
Expected
Not recommended
Tested
Not recommended
Not recommended
Rat
Expected
Expected
Not recommended
Tested
Not recommended
Not recommended
Synthetic peptide - Human
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Synthetic peptide - Mouse
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Synthetic peptide - Rat
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Synthetic peptide - Human, Synthetic peptide - Mouse, Synthetic peptide - Rat

Dilution info

-

Notes

-

Expected
Expected

Species

Human, Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide - Human, Synthetic peptide - Mouse, Synthetic peptide - Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Human, Rat, Synthetic peptide - Human, Synthetic peptide - Mouse, Synthetic peptide - Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide - Human, Synthetic peptide - Mouse, Synthetic peptide - Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human, Synthetic peptide - Human, Synthetic peptide - Mouse, Synthetic peptide - Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Human, Rat, Synthetic peptide - Human, Synthetic peptide - Mouse, Synthetic peptide - Rat

Dilution info

-

Notes

-

Target data

Function

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. Serves as a corepressor of RARA and causes its deacetylation (PubMed:28167758). In association with RARA, plays a role in the repression of microRNA-10a and thereby in the inflammatory response (PubMed:28167758).

Targets

HDAC4 phospho S246, HDAC7 phospho S155

Alternative names

Recommended products

Rabbit Recombinant Monoclonal HDAC5 phospho S259 antibody. Carrier free. Suitable for Dot, IP, WB and reacts with Synthetic peptide - Human, Synthetic peptide - Mouse, Synthetic peptide - Rat, Human, Mouse, Rat samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR22997-46

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab264560 is the carrier-free version of Anti-HDAC4 (phospho S246) + HDAC5 (phospho S259) + HDAC7 (phospho S155) antibody [EPR22997-46] ab240643.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

HDAC4 HDAC5 and HDAC7 are class IIa histone deacetylases. These proteins remove acetyl groups from lysine residues on histones leading to chromatin condensation and transcriptional repression. HDAC4 has a molecular mass of about 140 kDa HDAC5 around 122 kDa and HDAC7 approximately 107 kDa. They express predominantly in heart skeletal muscle and brain tissues. The proteins operate in both the nucleus and cytoplasm but they shuttle between these compartments regulated by phosphorylation.

Biological function summary

Histone deacetylases HDAC4 HDAC5 and HDAC7 play roles in gene expression modulation. They form complexes with transcription factors such as MEF2 (myocyte enhancer factor 2) integral in muscle differentiation processes. These HDACs influence cell cycle progression differentiation and survival by affecting the acetylation status of non-histone proteins. Their ability to silence specific gene sets makes them key regulators of cellular functions under different physiological and stress conditions.

Pathways

HDAC4 HDAC5 and HDAC7 participate in critical signaling events such as the MEF2 and NF-κB pathways. These HDACs interact with proteins in these pathways to modulate inflammation apoptosis and cardiac hypertrophy. They regulate gene expression by altering chromatin structure and functioning as transcription corepressors linking them to diverse cellular responses. Their activity connects closely with kinases like calcium/calmodulin-dependent protein kinase (CaMK) which influences their localization and activity.

Associated diseases and disorders

HDAC4 HDAC5 and HDAC7 are associated with cardiovascular disease and certain cancers. Aberrant activity or expression of these HDACs links to pathological cardiac remodeling as they regulate genes that affect heart muscle pathology. In cancer they govern processes linked to tumor progression like cell proliferation and survival. Abnormal interactions with proteins such as p53 and MEF2 in the context of these diseases highlight their importance in pathogenesis and potential as therapeutic targets.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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