Rabbit Polyclonal HDAC5 antibody. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human HDAC5 aa 500-550.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human HDAC5 aa 500-550. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UQL6
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Chimpanzee | Predicted | Predicted |
| Chinese hamster | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Gorilla | Predicted | Predicted |
| Horse | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
| Pig | Predicted | Predicted |
| Rabbit | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-10.00000 µg/mg of lysate | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan | Dilution info - | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. Serves as a corepressor of RARA and causes its deacetylation (PubMed:28167758). In association with RARA, plays a role in the repression of microRNA-10a and thereby in the inflammatory response (PubMed:28167758).
Alternative names
KIAA0600, HDAC5, Histone deacetylase 5, HD5, Antigen NY-CO-9
Recommended products
Rabbit Polyclonal HDAC5 antibody. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human HDAC5 aa 500-550.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human HDAC5 aa 500-550. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UQL6
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab225906 was affinity purified using an epitope specific to HDAC5 immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
HDAC5 also known as histone deacetylase 5 is an enzyme that removes acetyl groups from histone proteins leading to alteration of chromosome structure and regulation of gene expression. Its molecular weight is approximately 122 kDa. HDAC5 belongs to the class IIa HDAC family and shows high expression in heart brain and skeletal muscle. This expression pattern suggests its importance in these tissues. The role of HDAC5 in dynamically regulating chromatin structure positions it as an important modulator in the tight control of gene transcription.
- Biological function summary
HDAC5 plays a pivotal role in gene silencing and is a member of multi-protein complexes. It often partners with other HDACs and co-repressor proteins to enforce transcriptional repression. Through its activity HDAC5 affects cellular processes such as cell growth differentiation and apoptosis. Its expression and activity levels fluctuate among different cell types reflecting its involvement in tissue-specific functions and responses to cellular cues.
- Pathways
HDAC5 participates in critical signaling cascades like the MEF2 and NF-kB pathways. In the MEF2 pathway HDAC5 interacts closely with the MEF2 transcription factors repressing genes involved in muscle differentiation. Within the NF-kB pathway HDAC5 contributes to the regulation of inflammatory responses by modulating gene expression. The intricate relationship between HDAC5 and these pathways highlights its regulative influence on cellular functions and adaptive responses.
- Associated diseases and disorders
Recent research implicates HDAC5 in cancer and cardiac hypertrophy. Its overactivity or dysregulation has been observed in certain cancers where it may aid tumor progression by promoting oncogenic gene expression. Similarly in cardiac hypertrophy HDAC5 dysregulation can lead to maladaptive heart muscle growth a condition often exacerbated through interactions with protein kinases like CaMK. These associations have spurred interest in developing HDAC5 inhibitors as potential therapeutic targets to modulate its activity and address these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
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2 product images
Western blot - Anti-HDAC5 antibody (ab225906)
All lanes: Western blot - Anti-HDAC5 antibody (ab225906) at 0.04 µg/mL
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 4: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 121 kDa
Exposure time: 3min
Immunoprecipitation - Anti-HDAC5 antibody (ab225906)
HDAC5 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225906 at 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab225906 at 0.4 μg/ml.
Lane 1: ab225906 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
All lanes: Immunoprecipitation - Anti-HDAC5 antibody (ab225906)
Predicted band size: 121 kDa
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