AB55403

Anti-HDAC5 antibody

Name: Anti-HDAC5 antibody (ab55403) | Abcam
Brand: Abcam Limited
SKU: AB55403
Price: 435 USD
Availability: InStock
Rating: 3 (4 reviews)

(4 Reviews)

(24 Publications)

Rabbit Polyclonal HDAC5 antibody. Suitable for ELISA, WB, IHC-FoFr, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 24 publications. Immunogen corresponding to Synthetic Peptide within Human HDAC5.

View Alternative Names

KIAA0600, HDAC5, Histone deacetylase 5, HD5, Antigen NY-CO-9

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC5 antibody (AB55403)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC5 antibody (AB55403)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab55403 (1/50 dilution) in the presence and absence of the immunizing peptide.

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Western blot - Anti-HDAC5 antibody (AB55403)

All lanes:

Western blot - Anti-HDAC5 antibody (ab55403) at 1/500 dilution

Lane 1:

HepG2 cell extract, without immunizing peptide

Lane 2:

HepG2 cell extract, with immunizing peptide

Predicted band size: 121 kDa

Observed band size: 122 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

IHC-FoFr, WB, IHC-P, ICC/IF, ELISA

applications

Immunogen

Synthetic Peptide within Human HDAC5. The exact immunogen used to generate this antibody is proprietary information.

Q9UQL6

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab55403 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HDAC5 also known as histone deacetylase 5 is an enzyme that removes acetyl groups from histone proteins leading to alteration of chromosome structure and regulation of gene expression. Its molecular weight is approximately 122 kDa. HDAC5 belongs to the class IIa HDAC family and shows high expression in heart brain and skeletal muscle. This expression pattern suggests its importance in these tissues. The role of HDAC5 in dynamically regulating chromatin structure positions it as an important modulator in the tight control of gene transcription.

Biological function summary

HDAC5 plays a pivotal role in gene silencing and is a member of multi-protein complexes. It often partners with other HDACs and co-repressor proteins to enforce transcriptional repression. Through its activity HDAC5 affects cellular processes such as cell growth differentiation and apoptosis. Its expression and activity levels fluctuate among different cell types reflecting its involvement in tissue-specific functions and responses to cellular cues.

Pathways

HDAC5 participates in critical signaling cascades like the MEF2 and NF-kB pathways. In the MEF2 pathway HDAC5 interacts closely with the MEF2 transcription factors repressing genes involved in muscle differentiation. Within the NF-kB pathway HDAC5 contributes to the regulation of inflammatory responses by modulating gene expression. The intricate relationship between HDAC5 and these pathways highlights its regulative influence on cellular functions and adaptive responses.

Recent research implicates HDAC5 in cancer and cardiac hypertrophy. Its overactivity or dysregulation has been observed in certain cancers where it may aid tumor progression by promoting oncogenic gene expression. Similarly in cardiac hypertrophy HDAC5 dysregulation can lead to maladaptive heart muscle growth a condition often exacerbated through interactions with protein kinases like CaMK. These associations have spurred interest in developing HDAC5 inhibitors as potential therapeutic targets to modulate its activity and address these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. Serves as a corepressor of RARA and causes its deacetylation (PubMed : 28167758). In association with RARA, plays a role in the repression of microRNA-10a and thereby in the inflammatory response (PubMed : 28167758).

See full target information HDAC5

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Publications (24)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:1209 PubMed40804340

2025

rTMS ameliorates cerebral ischemia-reperfusion injury by inhibiting Golgi apparatus stress through epigenetic modulation of Gli2.

Applications

Unspecified application

Species

Unspecified reactive species

Chunjiao Zhu,Yongmei Fan,Wenna Peng

Respiratory research 25:227 PubMed38812021

2024

Expression of glucocorticoid receptor and HDACs in airway smooth muscle cells is associated with response to steroids in COPD.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Zhou,Michael Roth,Eleni Papakonstantinou,Michael Tamm,Daiana Stolz

Biomedicines 12: PubMed38790909

2024

Clinical Significance of the Immunohistochemical Expression of Histone Deacetylases (HDACs)-2, -4, and -5 in Ovarian Adenocarcinomas.

Applications

Unspecified application

Species

Unspecified reactive species

Georgia Levidou,Dimitrios Arsenakis,Dimitrios I Bolovis,Roxanne Meyer,Cosima V M Brucker,Thomas Papadopoulos,Stamatios Theocharis

JCI insight 8: PubMed37870958

2023

Comparative study in estrogen-depleted mice identifies skeletal and osteocyte transcriptomic responses to abaloparatide and teriparatide.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengtao Lv,Jiaming Zhang,Shuang Liang,Chenhe Zhou,Dorothy Hu,Daniel J Brooks,Mary L Bouxsein,Beate Lanske,Paul Kostenuik,Francesca Gori,Roland Baron

The journal of pain 25:428-450 PubMed37777035

2023

Epigenetic HDAC5 Inhibitor Reverses Craniofacial Neuropathic Pain in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Karin N Westlund,Marena Montera,Aleyah E Goins,Mark W Shilling,Mitra Afaghpour-Becklund,Sascha R A Alles,S Elise Hui

Science advances 9:eade4110 PubMed37018396

2023

An IL-6/STAT3/MR/FGF21 axis mediates heart-liver cross-talk after myocardial infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Jian-Yong Sun,Lin-Juan Du,Xue-Rui Shi,Yu-Yao Zhang,Yuan Liu,Yong-Li Wang,Bo-Yan Chen,Ting Liu,Hong Zhu,Yan Liu,Cheng-Chao Ruan,Zhenji Gan,Hao Ying,Zhinan Yin,Ping-Jin Gao,Xiaoxiang Yan,Ruo-Gu Li,Sheng-Zhong Duan

Frontiers in microbiology 14:1121863 PubMed36819033

2023

promotes lung adenocarcinoma progression by upregulating histone deacetylase 5.

Applications

Unspecified application

Species

Unspecified reactive species

Jiyu Shen,Yalan Ni,Qijie Guan,Rui Li,Hong Cao,Yan Geng,Qingjun You

International journal of biological sciences 18:5724-5739 PubMed36263180

2022

HDAC5-mediated Smad7 silencing through MEF2A is critical for fibroblast activation and hypertrophic scar formation.

Applications

Unspecified application

Species

Unspecified reactive species

Ya Gao,Yangdan Liu,Danning Zheng,Chiakang Ho,Dongsheng Wen,Jiaming Sun,Lu Huang,Yuxin Liu,Qingfeng Li,Yifan Zhang

Molecular neurobiology 59:6141-6157 PubMed35871708

2022

HDAC7 Activates IKK/NF-κB Signaling to Regulate Astrocyte-Mediated Inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Jinwang Ye,Suyue Zhong,Yunsong Deng,Xuanbao Yao,Qiong Liu,Jian-Zhi Wang,Shifeng Xiao

Cell biochemistry and function 40:589-599 PubMed35789099

2022

HDACs regulate the differentiation of endothelial cells from human iPSCs.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Li,Haopeng Wu,Pingping Wang,Amy M Kim,Junjing Jia,Jan A Nolta,Ping Zhou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HDAC5 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC5 antibody (AB55403)

Western blot - Anti-HDAC5 antibody (AB55403)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (24)

Product promise