AB327125

Anti-HDAC5 Antibody [CL8121]

Name: Anti-HDAC5 Antibody [CL8121] (ab327125) | Abcam
Brand: Abcam Limited
SKU: AB327125
Price: 580 USD
Availability: InStock

Be the first to review this product! Submit a review

(0 Publication)

Mouse Monoclonal HDAC5 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human HDAC5.

View Alternative Names

KIAA0600, HDAC5, Histone deacetylase 5, HD5, Antigen NY-CO-9

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC5 Antibody [CL8121] (AB327125)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC5 Antibody [CL8121] (AB327125)

Immunohistochemical staining of human placenta using ab327125 at 1 : 20–1 : 50 dilution, showing moderate to strong nuclear positivity in trophoblastic cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC5 Antibody [CL8121] (AB327125)

Immunohistochemical staining of human testis using ab327125 at 1 : 20–1 : 50 dilution, showing moderate to strong nuclear positivity in cells in seminiferous ducts.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC5 Antibody [CL8121] (AB327125)

Immunohistochemistry analysis in human cerebral cortex and liver tissues using ab327125 at 1 : 20–1 : 50 dilution. Corresponding HDAC5 RNA-seq data are presented for the same tissues.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC5 Antibody [CL8121] (AB327125)

Immunohistochemical staining of human stomach cancer using ab327125 at 1 : 20–1 : 50 dilution, showing moderate to strong cytoplasmic and nuclear positivity.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC5 Antibody [CL8121] (AB327125)

Immunohistochemical staining of human cerebral cortex using ab327125 at 1 : 20–1 : 50 dilution, showing strong nuclear positivity in neurons.

Immunocytochemistry/ Immunofluorescence - Anti-HDAC5 Antibody [CL8121] (AB327125)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HDAC5 Antibody [CL8121] (AB327125)

Immunofluorescence staining of RH-30 cells using ab327125 at 2–10 µg/ml, showing specific staining in the nucleoplasm and cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC5 Antibody [CL8121] (AB327125)

Immunohistochemical staining of human liver using ab327125 at 1 : 20–1 : 50 dilution, showing no positivity in hepatocytes as expected.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL8121

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Synthetic Peptide within Human HDAC5.

Q9UQL6

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2-10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

style

left-column

style

left-column

Properties and storage information

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. Serves as a corepressor of RARA and causes its deacetylation (PubMed : 28167758). In association with RARA, plays a role in the repression of microRNA-10a and thereby in the inflammatory response (PubMed : 28167758).

See full target information HDAC5

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com