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Rabbit Monoclonal HDAC5 antibody. Suitable for Dot, WB and reacts with Tag - Mouse, Human, Mouse, Rat samples.

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Images

Dot Blot - Anti-HDAC5 antibody [EPR29216-705] (AB322710), expandable thumbnail
  • Western blot - Anti-HDAC5 antibody [EPR29216-705] (AB322710), expandable thumbnail
  • Western blot - Anti-HDAC5 antibody [EPR29216-705] (AB322710), expandable thumbnail
  • Western blot - Anti-HDAC5 antibody [EPR29216-705] (AB322710), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
DotWBICC/IFIHC-P
Human
Expected
Tested
Not recommended
Not recommended
Mouse
Expected
Tested
Not recommended
Not recommended
Rat
Expected
Tested
Not recommended
Not recommended
Tag - Mouse
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Tag - Mouse
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Human, Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Tag - Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat, Tag - Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Tag - Mouse
Dilution info
-
Notes

-

Target data

Function

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors (By similarity). Serves as a corepressor of RARA and causes its deacetylation (By similarity). In association with RARA, plays a role in the repression of microRNA-10a and thereby in the inflammatory response (By similarity).

Additional Targets

HDAC5, Hdac5

Alternative names

Histone deacetylase 5, HD5, Histone deacetylase mHDA1

Rabbit Monoclonal HDAC5 antibody. Suitable for Dot, WB and reacts with Tag - Mouse, Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR29216-705
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Dot Blot - Anti-HDAC5 antibody [EPR29216-705] (ab322710), expandable thumbnail

    Dot Blot - Anti-HDAC5 antibody [EPR29216-705] (ab322710)

    Dot blot analysis of HDAC5 using ab322710 at 1:1000 (0.499 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.

    Lane1: His-tagged mouse HDAC5 recombinant protein

    Lane2: His-tagged mouse HDAC4 recombinant protein

    Lane3: His-tagged mouse HDAC7 recombinant protein

    Lane4: His-tagged mouse HDAC9 recombinant protein

    Exposure time: 180 seconds.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This antibody does not cross-react with mouse HDAC4, HDAC7 and HDAC9.

    In Dot Blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

    All lanes: Dot Blot - Anti-HDAC5 antibody [EPR29216-705] (ab322710) at 1/1000 dilution

    Lane 1: His-tagged mouse HDAC5 recombinant protein

    Lane 2: His-tagged mouse HDAC4 recombinant protein

    Lane 3: His-tagged mouse HDAC7 recombinant protein

    Lane 4: His-tagged mouse HDAC9 recombinant protein

    Secondary

    All lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Exposure time: 180s

  • Western blot - Anti-HDAC5 antibody [EPR29216-705] (ab322710), expandable thumbnail

    Western blot - Anti-HDAC5 antibody [EPR29216-705] (ab322710)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    To minimize protein degradation, cells/tissues were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

    This blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

    Exposure time: Lanes 1-2: 103 seconds, Lane 3: 180 seconds

    All lanes: Western blot - Anti-HDAC5 antibody [EPR29216-705] (ab322710) at 1/1000 dilution

    Lane 1: Mouse embryo brain tissue lysate at 50 µg

    Lane 2: Rat embryo brain tissue lysate at 50 µg

    Lane 3: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 100 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Observed band size: 140 kDa

  • Western blot - Anti-HDAC5 antibody [EPR29216-705] (ab322710), expandable thumbnail

    Western blot - Anti-HDAC5 antibody [EPR29216-705] (ab322710)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    To minimize protein degradation, cells/tissues were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

    The identity of the lower MW band at approximately 30 kDa (in lanes 1-2) is unknown.

    Exposure time: Lanes 1-2: 136 seconds, Lanes 3-4: 180 seconds.

    All lanes: Western blot - Anti-HDAC5 antibody [EPR29216-705] (ab322710) at 1/1000 dilution

    Lane 1: U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg

    Lane 3: MEF (mouse embryo fibroblast) whole cell lysate at 60 µg

    Lane 4: RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 140 kDa

  • Western blot - Anti-HDAC5 antibody [EPR29216-705] (ab322710), expandable thumbnail

    Western blot - Anti-HDAC5 antibody [EPR29216-705] (ab322710)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    To minimize protein degradation, cells/tissues were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-HDAC5 antibody [EPR29216-705] (ab322710) at 1/1000 dilution

    Lane 1: NIH/3T3 (mouse embryonic fibroblast) transfected with scrambled siRNA control whole cell lysate at 20 µg

    Lane 2: NIH/3T3 transfected with siRNA specifically targeting HDAC5 whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 140 kDa, 26 kDa

    Exposure time: 48s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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