AB50001

Anti-HDAC5 antibody [HDAC5-35]

Name: Anti-HDAC5 antibody [HDAC5-35] (ab50001) | Abcam
Brand: Abcam Limited
SKU: AB50001

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(3 Publications)

Mouse Monoclonal HDAC5 antibody. Suitable for WB and reacts with Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human HDAC5 aa 1-50 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

KIAA0600, HDAC5, Histone deacetylase 5, HD5, Antigen NY-CO-9

1 Images

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Western blot - Anti-HDAC5 antibody [HDAC5-35] (AB50001)

This image was generated using the ascites version of the product.

Lane 1:

Western blot - Anti-HDAC5 antibody [HDAC5-35] (ab50001) at 2 µg/mL

Lane 2:

no primary antibody

All lanes:

3T3 cell extract (mouse fibroblasts)

Secondary

All lanes:

Goat Anti-Mouse, Peroxidase conjugate

Predicted band size: 121 kDa

Observed band size: ~122 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

HDAC5-35

Isotype

IgG1

Carrier free

Reacts with

Mouse

Applications

applications

Immunogen

Synthetic Peptide within Human HDAC5 aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q9UQL6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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Product details

This product was changed from ascites to tissue culture supernatant on 05/06/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HDAC5 also known as histone deacetylase 5 is an enzyme that removes acetyl groups from histone proteins leading to alteration of chromosome structure and regulation of gene expression. Its molecular weight is approximately 122 kDa. HDAC5 belongs to the class IIa HDAC family and shows high expression in heart brain and skeletal muscle. This expression pattern suggests its importance in these tissues. The role of HDAC5 in dynamically regulating chromatin structure positions it as an important modulator in the tight control of gene transcription.

Biological function summary

HDAC5 plays a pivotal role in gene silencing and is a member of multi-protein complexes. It often partners with other HDACs and co-repressor proteins to enforce transcriptional repression. Through its activity HDAC5 affects cellular processes such as cell growth differentiation and apoptosis. Its expression and activity levels fluctuate among different cell types reflecting its involvement in tissue-specific functions and responses to cellular cues.

Pathways

HDAC5 participates in critical signaling cascades like the MEF2 and NF-kB pathways. In the MEF2 pathway HDAC5 interacts closely with the MEF2 transcription factors repressing genes involved in muscle differentiation. Within the NF-kB pathway HDAC5 contributes to the regulation of inflammatory responses by modulating gene expression. The intricate relationship between HDAC5 and these pathways highlights its regulative influence on cellular functions and adaptive responses.

Recent research implicates HDAC5 in cancer and cardiac hypertrophy. Its overactivity or dysregulation has been observed in certain cancers where it may aid tumor progression by promoting oncogenic gene expression. Similarly in cardiac hypertrophy HDAC5 dysregulation can lead to maladaptive heart muscle growth a condition often exacerbated through interactions with protein kinases like CaMK. These associations have spurred interest in developing HDAC5 inhibitors as potential therapeutic targets to modulate its activity and address these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. Serves as a corepressor of RARA and causes its deacetylation (PubMed : 28167758). In association with RARA, plays a role in the repression of microRNA-10a and thereby in the inflammatory response (PubMed : 28167758).

See full target information HDAC5

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Proceedings of the National Academy of Sciences of the United States of America 120:e2210953120 PubMed36745812

2023

Epigenetic function during heroin self-administration controls future relapse-associated behavior in a cell type-specific manner.

Applications

Unspecified application

Species

Unspecified reactive species

Ethan M Anderson,Evgeny Tsvetkov,Allison Galante,Derek DeVries,Lauren M McCue,Daniel Wood,Sarah Barry,Stefano Berto,Antonieta Lavin,Makoto Taniguchi,Christopher W Cowan

Cell death & disease 11:1043 PubMed33293505

2020

LncRNA kcnq1ot1 promotes lipid accumulation and accelerates atherosclerosis via functioning as a ceRNA through the miR-452-3p/HDAC3/ABCA1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Hua Yu,Wen-Yi Deng,Jiao-Jiao Chen,Xiao-Dan Xu,Xian-Xia Liu,Lei Chen,Meng-Wen Shi,Qi-Xian Liu,Min Tao,Kun Ren

Proceedings of the National Academy of Sciences of 112:E5088-97 PubMed26305935

2015

RGS9-2--controlled adaptations in the striatum determine the onset of action and efficacy of antidepressants in neuropathic pain states.

Applications

Species

Mouse

Vasiliki Mitsi,Dimitra Terzi,Immanuel Purushothaman,Lefteris Manouras,Sevasti Gaspari,Rachael L Neve,Maria Stratinaki,Jian Feng,Li Shen,Venetia Zachariou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HDAC5 antibody [HDAC5-35]

Western blot - Anti-HDAC5 antibody [HDAC5-35] (AB50001)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise