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AB264561

Anti-HDAC5 (phospho S498) antibody [EPR22996-105] - BSA and Azide free

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Rabbit Recombinant Monoclonal HDAC5 phospho S498 antibody. Carrier free. Suitable for Dot, WB and reacts with Synthetic peptide, Mouse, Human samples.

View Alternative Names

KIAA0600, HDAC5, Histone deacetylase 5, HD5, Antigen NY-CO-9

1 Images
Dot Blot - Anti-HDAC5 (phospho S498) antibody [EPR22996-105] - BSA and Azide free (AB264561)
  • Dot

Supplier Data

Dot Blot - Anti-HDAC5 (phospho S498) antibody [EPR22996-105] - BSA and Azide free (AB264561)

Dot blot analysis of HDAC5 (phospho S498) labeled with ab240644 at 1/1000 dilution.

Lane 1 : HDAC5 (phospho S498) peptide (aa495-504).

Lane 2 : HDAC5 non-phospho peptide (aa493-504).

Lane 3 : HDAC4 (phospho S632) peptide (aa627-638).

Lane 4 : HDAC7 (phospho S486) peptide (aa481-492).

Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution was used as secondary antibody.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, Azide, Glycerol and BSA (ab240644).

  • Unconjugated

    Anti-HDAC5 (phospho S498) antibody [EPR22996-105]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22996-105

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

WB, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Synthetic peptide": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

ab264561 is the carrier-free version of ab240644.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HDAC5 also known as histone deacetylase 5 is an enzyme that removes acetyl groups from histone proteins leading to alteration of chromosome structure and regulation of gene expression. Its molecular weight is approximately 122 kDa. HDAC5 belongs to the class IIa HDAC family and shows high expression in heart brain and skeletal muscle. This expression pattern suggests its importance in these tissues. The role of HDAC5 in dynamically regulating chromatin structure positions it as an important modulator in the tight control of gene transcription.
Biological function summary

HDAC5 plays a pivotal role in gene silencing and is a member of multi-protein complexes. It often partners with other HDACs and co-repressor proteins to enforce transcriptional repression. Through its activity HDAC5 affects cellular processes such as cell growth differentiation and apoptosis. Its expression and activity levels fluctuate among different cell types reflecting its involvement in tissue-specific functions and responses to cellular cues.

Pathways

HDAC5 participates in critical signaling cascades like the MEF2 and NF-kB pathways. In the MEF2 pathway HDAC5 interacts closely with the MEF2 transcription factors repressing genes involved in muscle differentiation. Within the NF-kB pathway HDAC5 contributes to the regulation of inflammatory responses by modulating gene expression. The intricate relationship between HDAC5 and these pathways highlights its regulative influence on cellular functions and adaptive responses.

Recent research implicates HDAC5 in cancer and cardiac hypertrophy. Its overactivity or dysregulation has been observed in certain cancers where it may aid tumor progression by promoting oncogenic gene expression. Similarly in cardiac hypertrophy HDAC5 dysregulation can lead to maladaptive heart muscle growth a condition often exacerbated through interactions with protein kinases like CaMK. These associations have spurred interest in developing HDAC5 inhibitors as potential therapeutic targets to modulate its activity and address these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. Serves as a corepressor of RARA and causes its deacetylation (PubMed : 28167758). In association with RARA, plays a role in the repression of microRNA-10a and thereby in the inflammatory response (PubMed : 28167758).
See full target information HDAC5 pS498
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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