Anti-HDAC6 antibody [EPR1698(2)] - BSA and Azide free (ab210472)

Rabbit Recombinant Monoclonal HDAC6 antibody. Carrier free. Suitable for ChIC/CUT&RUN-seq, IHC-P, WB, Flow Cyt (Intra) and reacts with Human, African green monkey samples.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-HDAC6 antibody [EPR1698(2)] - BSA and Azide free (AB210472), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	ChIC/CUT&RUN-seq	IHC-P	WB	Flow Cyt (Intra)
Human	Tested	Tested	Tested	Tested
African green monkey	Expected	Expected	Tested	Expected
Monkey	Predicted	Predicted	Predicted	Predicted

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species African green monkey	Dilution info Use at an assay dependent concentration.	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Monkey	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species African green monkey	Dilution info Use at an assay dependent concentration.	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Monkey	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human, African green monkey	Dilution info -	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Monkey	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species African green monkey	Dilution info Use at an assay dependent concentration.	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Monkey	Dilution info -	Notes -

Associated Products

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Target data

See full target information HDAC6

Function

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) (PubMed:10220385). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events (PubMed:10220385). Histone deacetylases act via the formation of large multiprotein complexes (PubMed:10220385). In addition to histones, deacetylates other proteins, such as CTTN, tubulin and SQSTM1 (PubMed:12024216, PubMed:20308065, PubMed:26246421, PubMed:30538141, PubMed:31857589). Plays a central role in microtubule-dependent cell motility by mediating deacetylation of tubulin (PubMed:12024216, PubMed:20308065, PubMed:26246421). Required for cilia disassembly; via deacetylation of alpha-tubulin (PubMed:17604723, PubMed:26246421). Promotes deacetylation of CTTN, leading to actin polymerization, promotion of autophagosome-lysosome fusion and completion of autophagy (PubMed:30538141). Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer (PubMed:24413532). Promotes odontoblast differentiation following IPO7-mediated nuclear import and subsequent repression of RUNX2 expression (By similarity). In addition to its protein deacetylase activity, plays a key role in the degradation of misfolded proteins: when misfolded proteins are too abundant to be degraded by the chaperone refolding system and the ubiquitin-proteasome, mediates the transport of misfolded proteins to a cytoplasmic juxtanuclear structure called aggresome (PubMed:17846173). Probably acts as an adapter that recognizes polyubiquitinated misfolded proteins and target them to the aggresome, facilitating their clearance by autophagy (PubMed:17846173).

Alternative names

KIAA0901, JM21, HDAC6, Histone deacetylase 6, HD6, Protein deacetylase HDAC6, Tubulin-lysine deacetylase HDAC6

Recommended products

Rabbit Recombinant Monoclonal HDAC6 antibody. Carrier free. Suitable for ChIC/CUT&RUN-seq, IHC-P, WB, Flow Cyt (Intra) and reacts with Human, African green monkey samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR1698(2)
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: Do Not Freeze

Notes

ab210472 is the carrier-free version of Anti-HDAC6 antibody [EPR1698(2)] ab133493.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

HDAC6 or histone deacetylase 6 is a protein that primarily functions as a cytoplasmic deacetylase. It is part of the class IIb HDAC family and is known for its distinctive molecular weight of approximately 121 kDa. HDAC6 is expressed in various tissues with higher levels observed in the brain kidney and liver. This protein is unique as it contains two catalytic domains unlike other HDACs which contributes to its specific deacetylation of non-histone substrates including tubulin and Hsp90 influencing cell motility and stress response.

Biological function summary

HDAC6 plays a significant role in processes like protein degradation and cell signaling. It is an important component of the protein quality control system involving itself in the aggresome pathway where it facilitates the removal of misfolded proteins through interaction with dynein motor proteins. In addition to its presence in the cytoplasm HDAC6 influences cell migration and immune response regulation by de-phosphorylating cortactin and affecting actin filament dynamics. Its integral role in the aggresome-autophagy pathway positions it as important for cellular homeostasis maintenance.

Pathways

HDAC6 participates prominently in both autophagy and stress response pathways. In the autophagic process HDAC6 operates alongside ubiquitinated proteins to manage protein quality control. Moreover HDAC6 engages in stress response pathways like the heat shock response interacting directly with Hsp90 to regulate client protein activation. These pathways highlight HDAC6’s relationships with key proteins such as Hsp70 and tau linking it to cellular stress and neurodegeneration responses.

Associated diseases and disorders

HDAC6 exhibits connections to neurodegenerative diseases and cancer. Dysregulated HDAC6 activity associates with Alzheimer's disease where it affects tau protein accumulation and degradation. The protein is also implicated in various cancers such as breast and ovarian cancer due to its influence on cell migration and invasion. It interacts with p53 impacting apoptosis and tumor progression making HDAC6 a potential target for therapeutic interventions with HDAC6 inhibitors which aim to restore normal cellular functions disrupted by abnormal HDAC6 activity.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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8 product images

Immunocytochemistry/ Immunofluorescence - Anti-HDAC6 antibody [EPR1698(2)] - BSA and Azide free (ab210472)
Clone EPR1698(2) (ab210472) has been successfully conjugated by Abcam. This image was generated using Anti-HDAC6 antibody [EPR1698(2)] (Alexa Fluor® 647). Please refer to Alexa Fluor® 647 Anti-HDAC6 antibody [EPR1698(2)] ab202833 for protocol details.
Alexa Fluor® 647 Anti-HDAC6 antibody [EPR1698(2)] ab202833 staining HDAC6 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with Alexa Fluor® 647 Anti-HDAC6 antibody [EPR1698(2)] ab202833 at a 1/100 dilution (shown in red) and Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 4% formaldehyde (10 min).
Immunocytochemistry/ Immunofluorescence - Anti-HDAC6 antibody [EPR1698(2)] - BSA and Azide free (ab210472)
Clone EPR1698(2) (ab210472) has been successfully conjugated by Abcam. This image was generated using Anti-HDAC6 antibody [EPR1698(2)] (Alexa Fluor® 488). Please refer to Alexa Fluor® 488 Anti-HDAC6 antibody [EPR1698(2)] ab202948 for protocol details.
Alexa Fluor® 488 Anti-HDAC6 antibody [EPR1698(2)] ab202948 staining HDAC6 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with Alexa Fluor® 488 Anti-HDAC6 antibody [EPR1698(2)] ab202948 at 1/200 dilution (shown in green) and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Western blot - Anti-HDAC6 antibody [EPR1698(2)] - BSA and Azide free (ab210472)
This data was developed using the same antibody clone in a different buffer formulation (Anti-HDAC6 antibody [EPR1698(2)] ab133493).
Lanes 1- 2: Merged signal (red and green). Green - Anti-HDAC6 antibody [EPR1698(2)] ab133493 observed at 160 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.
Anti-HDAC6 antibody [EPR1698(2)] ab133493 was shown to react with HDAC6 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab264804 (knockout cell lysate ab257145) was used. Wild-type HeLa and HDAC6 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. Anti-HDAC6 antibody [EPR1698(2)] ab133493 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-HDAC6 antibody [EPR1698(2)] (Anti-HDAC6 antibody [EPR1698(2)] ab133493) at 1/10000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: HDAC6 knockout HeLa cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 131 kDa
Observed band size: 160 kDa
Flow Cytometry (Intracellular) - Anti-HDAC6 antibody [EPR1698(2)] - BSA and Azide free (ab210472)
Anti-HDAC6 antibody [EPR1698(2)] ab133493 staining HDAC6 in K562 (human chronic myelogenous leukemia) by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde, permabilised with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/200. A goat anti rabbit IgG (Alexa Fluor^® 488) at a dilution of 1/2000 was used as the secondary antibody.
Isoytype control: Rabbit monoclonal IgG (Black)
Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-HDAC6 antibody [EPR1698(2)] ab133493).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC6 antibody [EPR1698(2)] - BSA and Azide free (ab210472)
Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling HDAC6 with Anti-HDAC6 antibody [EPR1698(2)] ab133493 antibody at a dilution of 1/50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-HDAC6 antibody [EPR1698(2)] ab133493).
Western blot - Anti-HDAC6 antibody [EPR1698(2)] - BSA and Azide free (ab210472)
This data was developed using the same antibody clone in a different buffer formulation (Anti-HDAC6 antibody [EPR1698(2)] ab133493).
Lanes 1 and 2: Green signal from target – Anti-HDAC6 antibody [EPR1698(2)] ab133493 observed at 160 kDa
Lanes 3 and 4: Red signal from loading control – Anti-beta Actin antibody [mAbcam 8226] - Loading Control ab8226 observed at 42 kDa
Lanes 5 and 6: Merged (red and green) signal
Anti-HDAC6 antibody [EPR1698(2)] ab133493 was shown to specifically react with HDAC6 when HDAC6 knockout samples were used. Wild-type and HDAC6 knockout samples were subjected to SDS-PAGE. Anti-HDAC6 antibody [EPR1698(2)] ab133493 and Anti-beta Actin antibody [mAbcam 8226] - Loading Control ab8226 (loading control to beta actin) were diluted 1/10 000 and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
Lanes 1 - 2: Western blot - Anti-HDAC6 antibody [EPR1698(2)] - BSA and Azide free (ab210472) at 1/10000 dilution
Lanes 3 - 4: Western blot - Anti-HDAC6 antibody [EPR1698(2)] - BSA and Azide free (ab210472) at 1/1000 dilution
Lanes 5 - 6: Western blot - Anti-HDAC6 antibody [EPR1698(2)] - BSA and Azide free (ab210472)
Lanes 1, 3 and 5: Wild-type HAP1 cell lysate at 20 µg
Lanes 2, 4 and 6: HDAC6 knockout HAP1 cell lysate at 20 µg
Predicted band size: 131 kDa
ChIC/CUT&RUN sequencing - Anti-HDAC6 antibody [EPR1698(2)] - BSA and Azide free (ab210472)
This data was developed using ab210472, the same antibody clone in a different buffer formulation.
ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 HeLa (human cervix adenocarcinoma epithelial cell) cells and 5 µg of Anti-HDAC6 antibody [EPR1698(2)] ab133493 [EPR1698(2)]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown.
The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.
ChIC/CUT&RUN sequencing - Anti-HDAC6 antibody [EPR1698(2)] - BSA and Azide free (ab210472)
This data was developed using Anti-HDAC6 antibody [EPR1698(2)] ab133493, the same antibody clone in a different buffer formulation.
ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 HeLa (human cervix adenocarcinoma epithelial cell) cells and 5 µg of Anti-HDAC6 antibody [EPR1698(2)] ab133493 [EPR1698(2)]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown.
The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

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Anti-HDAC6 antibody [EPR1698(2)] - BSA and Azide free

Images

Key facts

Immunogen

Reactivity data

ChIC/CUT&RUN-seq

TestedTested

ExpectedExpected

PredictedPredicted

IHC-P

TestedTested

ExpectedExpected

PredictedPredicted

WB

TestedTested

PredictedPredicted

Flow Cyt (Intra)

TestedTested

ExpectedExpected

PredictedPredicted

Associated Products

8 products for Alternative Product

3 products for Alternative Version

Target data

Function

Alternative names

Recommended products

Key facts

Storage

Notes

Supplementary info

Product promise

Tested

Expected

Predicted

Not recommended

8 product images

Immunocytochemistry/ Immunofluorescence - Anti-HDAC6 antibody [EPR1698(2)] - BSA and Azide free (ab210472)

Immunocytochemistry/ Immunofluorescence - Anti-HDAC6 antibody [EPR1698(2)] - BSA and Azide free (ab210472)

Western blot - Anti-HDAC6 antibody [EPR1698(2)] - BSA and Azide free (ab210472)

Flow Cytometry (Intracellular) - Anti-HDAC6 antibody [EPR1698(2)] - BSA and Azide free (ab210472)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC6 antibody [EPR1698(2)] - BSA and Azide free (ab210472)

Western blot - Anti-HDAC6 antibody [EPR1698(2)] - BSA and Azide free (ab210472)

ChIC/CUT&RUN sequencing - Anti-HDAC6 antibody [EPR1698(2)] - BSA and Azide free (ab210472)

ChIC/CUT&RUN sequencing - Anti-HDAC6 antibody [EPR1698(2)] - BSA and Azide free (ab210472)

Downloads

Product protocols

Tested
Tested

Expected
Expected

Predicted
Predicted

Tested
Tested

Expected
Expected

Predicted
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Tested
Tested

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