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Rabbit Recombinant Monoclonal HDAC6 antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Mouse, Rat samples. Cited in 1 publication.

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Images

Western blot - Anti-HDAC6 antibody [EPR22951-29] (AB239362), expandable thumbnail
  • Immunoprecipitation - Anti-HDAC6 antibody [EPR22951-29] (AB239362), expandable thumbnail
  • Western blot - Anti-HDAC6 antibody [EPR22951-29] (AB239362), expandable thumbnail
  • Immunoprecipitation - Anti-HDAC6 antibody [EPR22951-29] (AB239362), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-HDAC6 antibody [EPR22951-29] (AB239362), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-PICC/IFFlow Cyt (Intra)
Mouse
Tested
Tested
Not recommended
Not recommended
Tested
Rat
Expected
Tested
Not recommended
Not recommended
Expected

Tested
Tested

Species

Mouse

Dilution info

1/30

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/600

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) (PubMed:9891014). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events (PubMed:9891014). Histone deacetylases act via the formation of large multiprotein complexes (PubMed:9891014). In addition to histones, deacetylates other proteins: plays a central role in microtubule-dependent cell motility by mediating deacetylation of tubulin (PubMed:19893491). Promotes deacetylation of CTTN, leading to actin polymerization, promotion of autophagosome-lysosome fusion and completion of autophagy (By similarity). In addition to its protein deacetylase activity, plays a key role in the degradation of misfolded proteins: when misfolded proteins are too abundant to be degraded by the chaperone refolding system and the ubiquitin-proteasome, mediates the transport of misfolded proteins to a cytoplasmic juxtanuclear structure called aggresome (By similarity). Probably acts as an adapter that recognizes polyubiquitinated misfolded proteins and target them to the aggresome, facilitating their clearance by autophagy (PubMed:22819792).

Alternative names

Histone deacetylase 6, HD6, Histone deacetylase mHDA2, Tubulin-lysine deacetylase HDAC6, Hdac6

Recommended products

Rabbit Recombinant Monoclonal HDAC6 antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Mouse, Rat samples. Cited in 1 publication.

Alternative names

Histone deacetylase 6, HD6, Histone deacetylase mHDA2, Tubulin-lysine deacetylase HDAC6, Hdac6

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR22951-29

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

HDAC6 or histone deacetylase 6 is a protein that primarily functions as a cytoplasmic deacetylase. It is part of the class IIb HDAC family and is known for its distinctive molecular weight of approximately 121 kDa. HDAC6 is expressed in various tissues with higher levels observed in the brain kidney and liver. This protein is unique as it contains two catalytic domains unlike other HDACs which contributes to its specific deacetylation of non-histone substrates including tubulin and Hsp90 influencing cell motility and stress response.

Biological function summary

HDAC6 plays a significant role in processes like protein degradation and cell signaling. It is an important component of the protein quality control system involving itself in the aggresome pathway where it facilitates the removal of misfolded proteins through interaction with dynein motor proteins. In addition to its presence in the cytoplasm HDAC6 influences cell migration and immune response regulation by de-phosphorylating cortactin and affecting actin filament dynamics. Its integral role in the aggresome-autophagy pathway positions it as important for cellular homeostasis maintenance.

Pathways

HDAC6 participates prominently in both autophagy and stress response pathways. In the autophagic process HDAC6 operates alongside ubiquitinated proteins to manage protein quality control. Moreover HDAC6 engages in stress response pathways like the heat shock response interacting directly with Hsp90 to regulate client protein activation. These pathways highlight HDAC6’s relationships with key proteins such as Hsp70 and tau linking it to cellular stress and neurodegeneration responses.

Associated diseases and disorders

HDAC6 exhibits connections to neurodegenerative diseases and cancer. Dysregulated HDAC6 activity associates with Alzheimer's disease where it affects tau protein accumulation and degradation. The protein is also implicated in various cancers such as breast and ovarian cancer due to its influence on cell migration and invasion. It interacts with p53 impacting apoptosis and tumor progression making HDAC6 a potential target for therapeutic interventions with HDAC6 inhibitors which aim to restore normal cellular functions disrupted by abnormal HDAC6 activity.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-HDAC6 antibody [EPR22951-29] (ab239362), expandable thumbnail

    Western blot - Anti-HDAC6 antibody [EPR22951-29] (ab239362)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 18180281, 29201907, 28273454, 25108026).

    Exposure time: Lane 1-2: 32 seconds Lane 3: 3 minutes.

    All lanes: Western blot - Anti-HDAC6 antibody [EPR22951-29] (ab239362) at 1/1000 dilution

    Lane 1: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 10 µg

    Lane 2: Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate at 10 µg

    Lane 3: PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 131 kDa

    Observed band size: 130 kDa

  • Immunoprecipitation - Anti-HDAC6 antibody [EPR22951-29] (ab239362), expandable thumbnail

    Immunoprecipitation - Anti-HDAC6 antibody [EPR22951-29] (ab239362)

    HDAC6 was immunoprecipitated from 0.35 mg NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate 10μg with ab239362 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab239362 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: NIH/3T3 whole cell lysate 10μg

    Lane 2: ab239362 IP in NIH/3T3 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab239362 in NIH/3T3 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

    All lanes: Immunoprecipitation - Anti-HDAC6 antibody [EPR22951-29] (ab239362)

    Predicted band size: 131 kDa, 47 kDa

  • Western blot - Anti-HDAC6 antibody [EPR22951-29] (ab239362), expandable thumbnail

    Western blot - Anti-HDAC6 antibody [EPR22951-29] (ab239362)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Kidney is a low-expression tissue.

    A positive unidentified band between 15-20 kDa is detected in mouse kidney lysate.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 18180281, 29201907, 28273454, 25108026).

    Exposure time: 48 seconds.

    All lanes: Western blot - Anti-HDAC6 antibody [EPR22951-29] (ab239362) at 1/1000 dilution

    Lane 1: Mouse brain tissue lysate at 20 µg

    Lane 2: Mouse kidney tissue lysate at 20 µg

    Lane 3: Rat brain tissue lysate at 20 µg

    Lane 4: Rat kidney tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 131 kDa

    Observed band size: 130 kDa

  • Immunoprecipitation - Anti-HDAC6 antibody [EPR22951-29] (ab239362), expandable thumbnail

    Immunoprecipitation - Anti-HDAC6 antibody [EPR22951-29] (ab239362)

    HDAC6 was immunoprecipitated from 0.35 mg mouse brain lysate 10μg with ab239362 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab239362 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse brain lysate 10μg

    Lane 2: ab239362 IP in mouse brain lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab239362 in mouse brain lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 mins.

    All lanes: Immunoprecipitation - Anti-HDAC6 antibody [EPR22951-29] (ab239362)

    Predicted band size: 131 kDa

  • Flow Cytometry (Intracellular) - Anti-HDAC6 antibody [EPR22951-29] (ab239362), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-HDAC6 antibody [EPR22951-29] (ab239362)

    Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) cells labeling HDAC6 with ab239362 at 1\600 (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 was used as the secondary antibody.

  • Flow Cytometry (Intracellular) - Anti-HDAC6 antibody [EPR22951-29] (ab239362), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-HDAC6 antibody [EPR22951-29] (ab239362)

    Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Neuro-2a (Mouse neuroblastoma neuroblast) cells labeling HDAC6 with ab239362 at 1\600 (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 was used as the secondary antibody.

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