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AB325549

Anti-HDAC6 antibody [EPR29478-555] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal HDAC6 antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples.

View Alternative Names

Histone deacetylase 6, HD6, Histone deacetylase mHDA2, Protein deacetylase HDAC6, Tubulin-lysine deacetylase HDAC6, Hdac6

2 Images
Sandwich ELISA - Anti-HDAC6 antibody [EPR29478-555] - BSA and Azide free (Detector) (AB325549)
  • sELISA

Lab

Sandwich ELISA - Anti-HDAC6 antibody [EPR29478-555] - BSA and Azide free (Detector) (AB325549)

Interpolated concentration of native HDAC6 was measured at different sample concentrations. Undiluted samples are as follows : mouse kidney tissue extract (200 μg/mL) and NIH/3T3 cell extract (500 μg/mL). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in antibody diluent CP2 (ab319981). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

Sandwich ELISA - Anti-HDAC6 antibody [EPR29478-555] - BSA and Azide free (Detector) (AB325549)
  • sELISA

Lab

Sandwich ELISA - Anti-HDAC6 antibody [EPR29478-555] - BSA and Azide free (Detector) (AB325549)

Sandwich ELISA with the capture antibody ab325548 dilution at 2 μg/mL and detector antibody ab325549 dilution at 0.5 μg/mL.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR29478-555

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab325549 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse HDAC6. The recommended pair for sandwich ELISA is:

Capture: ab325548, Mouse HDAC6 Capture Antibody (unconjugated)

Detector: ab325549, Mouse HDAC6 Detector Antibody (unconjugated)

The reference range value is 313-20000 pg/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) (PubMed : 9891014). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events (PubMed : 9891014). Histone deacetylases act via the formation of large multiprotein complexes (PubMed : 9891014). In addition to histones, deacetylates other proteins, such as CTTN, tubulin and SQSTM1 (PubMed : 19893491, PubMed : 27737934). Plays a central role in microtubule-dependent cell motility by mediating deacetylation of tubulin (PubMed : 19893491, PubMed : 27737934). Required for cilia disassembly; via deacetylation of alpha-tubulin (By similarity). Promotes deacetylation of CTTN, leading to actin polymerization, promotion of autophagosome-lysosome fusion and completion of autophagy (By similarity). Promotes odontoblast differentiation following IPO7-mediated nuclear import and subsequent repression of RUNX2 expression (PubMed : 35922041). In addition to its protein deacetylase activity, plays a key role in the degradation of misfolded proteins : when misfolded proteins are too abundant to be degraded by the chaperone refolding system and the ubiquitin-proteasome, mediates the transport of misfolded proteins to a cytoplasmic juxtanuclear structure called aggresome (By similarity). Probably acts as an adapter that recognizes polyubiquitinated misfolded proteins and target them to the aggresome, facilitating their clearance by autophagy (PubMed : 22819792).
See full target information Hdac6
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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