AB12174

Anti-HDAC7 antibody

Name: Anti-HDAC7 antibody (ab12174) | Abcam
Brand: Abcam Limited
SKU: AB12174
Price: 430 USD
Availability: InStock
Rating: 4 (4 reviews)

(4 Reviews)

(19 Publications)

Rabbit Polyclonal HDAC7 antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 19 publications. Immunogen corresponding to Synthetic Peptide within Human HDAC7 aa 400 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

HDAC7A, HDAC7, Histone deacetylase 7, HD7, Histone deacetylase 7A, Protein deacetylase HDAC7, HD7a

2 Images

Supplier Data

Immunoprecipitation - Anti-HDAC7 antibody (AB12174)

HDAC7 was immunoprecipitated from whole cell extracts of human HEK-293T cells expressing HDAC7-FLAG using ab12174. The IP samples were separated on SDS-PAGE, blotted with ANTI-FLAG^® and developed using Anti-Rabbit IgG, Peroxidase and chemiluminescent substrate.

Lane 1 : 1.8 μg antibody immunoprecipitates HDAC7 from a RIPA extract of 180 μg of HEK-293T cells expressing HDAC7-FLAG
Lane 2 : Negative control (extract without antibody).

All lanes:

Immunoprecipitation - Anti-HDAC7 antibody (ab12174)

Predicted band size: 103 kDa

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Supplier Data

Western blot - Anti-HDAC7 antibody (AB12174)

All lanes:

Western blot - Anti-HDAC7 antibody (ab12174) at 1/500 dilution

Lane 1:

HeLa nuclear lysate

Lane 2:

NIH3T3 cell lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG-Peroxidase

Predicted band size: 103 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human, Rat

Applications

WB, IP, ICC/IF

applications

Immunogen

Synthetic Peptide within Human HDAC7 aa 400 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "1.0-1.5 μg of the antibody immunoprecipitates HDAC7 from a RIPA extract (180 μg) of 293T cells expressing recombinant human HDAC7.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "This dilution is recommended using whole extracts of mouse NIH-3T3 cells. Additional bands may be detected in various extract preparations. Detection of HDAC7 is specifically inhibited with the immunizing peptide." }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "This dilution is recommended using whole extracts of mouse NIH-3T3 cells. Additional bands may be detected in various extract preparations. Detection of HDAC7 is specifically inhibited with the immunizing peptide." }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "This dilution is recommended using whole extracts of mouse NIH-3T3 cells. Additional bands may be detected in various extract preparations. Detection of HDAC7 is specifically inhibited with the immunizing peptide." } } }

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Product details

Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

The antibody is affinity-purified using the immunizing peptide immobilized on agarose.

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Histone deacetylase 7 (HDAC7) also known as HD7 or HD7A is a part of the class IIa histone deacetylases. It has a mass of approximately 103 kDa. HDAC7 plays a role in regulating gene expression by removing acetyl groups from lysine residues on histones leading to chromatin condensation and gene repression. This protein is expressed in various tissues including the heart skeletal muscle and T-cells where it influences diverse cellular processes.

Biological function summary

HDAC7 modulates transcription and affects cell differentiation and survival. It is a component of a larger protein complex working together with other proteins to influence transcription factor activity and chromatin architecture. It influences the expression of genes involved in immune responses as well as other critical cellular activities. By regulating these processes HDAC7 contributes to the balance between cell proliferation and apoptosis.

Pathways

HDAC7 plays key roles in the Wnt signaling and T-cell receptor (TCR) signaling pathways. It interacts with other proteins such as HDAC3 and the nuclear receptor co-repressor (N-CoR) affecting pathway components and influencing cellular outcomes. In the Wnt pathway HDAC7's activity modifies the transcription of target genes impacting cell fate decisions. During TCR signaling HDAC7 aids in the regulation of T-cell development and function working closely with transcriptional factors involved in these pathways.

HDAC7 has been associated with cancer and cardiovascular diseases. Its dysregulation can contribute to tumorigenesis through aberrant expression of genes controlling cell cycle and apoptosis. In cardiovascular disorders it impacts cardiac hypertrophy and heart failure due to its influence on heart-specific gene expression. HDAC7’s interaction with proteins like RUNX1 in T-cells can also contribute to the development of leukemia. These links highlight the importance of HDAC7 in both disease development and progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) (By similarity). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events (By similarity). Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Involved in muscle maturation by repressing transcription of myocyte enhancer factors such as MEF2A, MEF2B and MEF2C (By similarity). During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors (By similarity). May be involved in Epstein-Barr virus (EBV) latency, possibly by repressing the viral BZLF1 gene (PubMed : 12239305). Positively regulates the transcriptional repressor activity of FOXP3 (PubMed : 17360565). Serves as a corepressor of RARA, causing its deacetylation and inhibition of RARE DNA element binding (PubMed : 28167758). In association with RARA, plays a role in the repression of microRNA-10a and thereby in the inflammatory response (PubMed : 28167758). Also acetylates non-histone proteins, such as ALKBH5 (PubMed : 37369679).

See full target information HDAC7

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Publications (19)

Recent publications for all applications. Explore the full list and refine your search

Mediators of inflammation 2025:8156593 PubMed40151316

2025

The Histone Deacetylase Activator ITSA-1 Improves the Prognosis of Cardiac Arrest Rats by Alleviating Systemic Inflammatory Responses Following Cardiopulmonary Resuscitation.

Applications

Unspecified application

Species

Unspecified reactive species

Chenyu Zhang,Hongyan Wei,Qiang Zhang,Haohong Zhan,Yuanzheng Lu,Yujie Li,Bo Li,Wen Huang,Feng Nian,Rong Liu,Chunlin Hu,Jie Chen

Journal of cellular and molecular medicine 28:e70120 PubMed39431349

2024

HDAC7 promotes ovarian cancer malignancy via AKT/mTOR signalling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Feng,Sheng Hao,Xiongxiu Liu,Zhong Yan,Kai Sheng,Yanping Li,Peng Zhang,Xiugui Sheng

Cartilage :19476035241264012 PubMed39057748

2024

Effects of Etanercept on Experimental Osteoarthritis in Rats: Role of Histone Deacetylases.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi-Hong Wen,Chi-Chieh Tang,Yen-You Lin,Zhi-Kang Yao,Shih-Peng Hsieh, Gar-Hwa-Lai,Wu-Fu Chen,Yen-Hsuan Jean

Cell death discovery 9:245 PubMed37452056

2023

Restricting epigenetic activity promotes the reprogramming of transformed cells to pluripotency in a line-specific manner.

Applications

Unspecified application

Species

Unspecified reactive species

Xiuling Fu,Qiang Zhuang,Isaac A Babarinde,Liyang Shi,Gang Ma,Haoqing Hu,Yuhao Li,Jiao Chen,Zhen Xiao,Boping Deng,Li Sun,Ralf Jauch,Andrew P Hutchins

Journal of medicinal chemistry 65:12445-12459 PubMed36098485

2022

Developing HDAC4-Selective Protein Degraders To Investigate the Role of HDAC4 in Huntington's Disease Pathology.

Applications

Unspecified application

Species

Unspecified reactive species

Natsuko Macabuag,William Esmieu,Perla Breccia,Rebecca Jarvis,Wesley Blackaby,Ovadia Lazari,Liudvikas Urbonas,Maria Eznarriaga,Rachel Williams,Annelieke Strijbosch,Rhea Van de Bospoort,Kim Matthews,Cole Clissold,Tammy Ladduwahetty,Huw Vater,Patrick Heaphy,Douglas G Stafford,Hong-Jun Wang,John E Mangette,George McAllister,Vahri Beaumont,Thomas F Vogt,Hilary A Wilkinson,Elizabeth M Doherty,Celia Dominguez

Cell death discovery 8:322 PubMed35840562

2022

N-methyladenosine (mA) methyltransferase METTL3 regulates sepsis-induced myocardial injury through IGF2BP1/HDAC4 dependent manner.

Applications

Species

Rat

Hao Shen,Keliang Xie,Miaomiao Li,Qianyu Yang,Xiaoye Wang

Nature communications 11:3282 PubMed32612176

2020

A FAK/HDAC5 signaling axis controls osteocyte mechanotransduction.

Applications

Unspecified application

Species

Unspecified reactive species

Tadatoshi Sato,Shiv Verma,Christian D Castro Andrade,Maureen Omeara,Nia Campbell,Jialiang S Wang,Murat Cetinbas,Audrey Lang,Brandon J Ausk,Daniel J Brooks,Ruslan I Sadreyev,Henry M Kronenberg,David Lagares,Yuhei Uda,Paola Divieti Pajevic,Mary L Bouxsein,Ted S Gross,Marc N Wein

Nature communications 10:5052 PubMed31699992

2019

Quantitating the epigenetic transformation contributing to cholesterol homeostasis using Gaussian process.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Wang,Samantha M Scott,Kanagaraj Subramanian,Salvatore Loguercio,Pei Zhao,Darren M Hutt,Nicole Y Farhat,Forbes D Porter,William E Balch

Oncogene 38:6599-6614 PubMed31375747

2019

HDAC7 regulates histone 3 lysine 27 acetylation and transcriptional activity at super-enhancer-associated genes in breast cancer stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Corrado Caslini,Sunhwa Hong,Yuguang J Ban,Xi S Chen,Tan A Ince

Human molecular genetics 28:1982-2000 PubMed30753450

2019

HDAC inhibitors rescue multiple disease-causing CFTR variants.

Applications

Unspecified application

Species

Unspecified reactive species

Frédéric Anglès,Darren M Hutt,William E Balch

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-HDAC7 antibody

Immunoprecipitation - Anti-HDAC7 antibody (AB12174)

Western blot - Anti-HDAC7 antibody (AB12174)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (19)

Product promise