AB137366

Anti-HDAC7 antibody - C-terminal

Name: Anti-HDAC7 antibody - C-terminal (ab137366) | Abcam
Brand: Abcam Limited
SKU: AB137366
Price: 545 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal HDAC7 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human HDAC7 aa 700 to C-terminus.

View Alternative Names

HDAC7A, HDAC7, Histone deacetylase 7, HD7, Histone deacetylase 7A, Protein deacetylase HDAC7, HD7a

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-HDAC7 antibody - C-terminal (AB137366)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-HDAC7 antibody - C-terminal (AB137366)

Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labelling HDAC7 (green) with ab137366 at 1/1000 dilution. The lower image was co-stained with Hoechst 33342 (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC7 antibody - C-terminal (AB137366)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC7 antibody - C-terminal (AB137366)

Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labelling HDAC7 with ab137366 at 1/250 dilution.

Supplier Data

Western blot - Anti-HDAC7 antibody - C-terminal (AB137366)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-HDAC7 antibody - C-terminal (ab137366) at 1/1000 dilution

All lanes:

HeLa whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human HDAC7 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8WUI4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "Antigen retrieval using heat mediated Tris-EDTA buffer (pH8.0) is also possible. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Histone deacetylase 7 (HDAC7) also known as HD7 or HD7A is a part of the class IIa histone deacetylases. It has a mass of approximately 103 kDa. HDAC7 plays a role in regulating gene expression by removing acetyl groups from lysine residues on histones leading to chromatin condensation and gene repression. This protein is expressed in various tissues including the heart skeletal muscle and T-cells where it influences diverse cellular processes.

Biological function summary

HDAC7 modulates transcription and affects cell differentiation and survival. It is a component of a larger protein complex working together with other proteins to influence transcription factor activity and chromatin architecture. It influences the expression of genes involved in immune responses as well as other critical cellular activities. By regulating these processes HDAC7 contributes to the balance between cell proliferation and apoptosis.

Pathways

HDAC7 plays key roles in the Wnt signaling and T-cell receptor (TCR) signaling pathways. It interacts with other proteins such as HDAC3 and the nuclear receptor co-repressor (N-CoR) affecting pathway components and influencing cellular outcomes. In the Wnt pathway HDAC7's activity modifies the transcription of target genes impacting cell fate decisions. During TCR signaling HDAC7 aids in the regulation of T-cell development and function working closely with transcriptional factors involved in these pathways.

HDAC7 has been associated with cancer and cardiovascular diseases. Its dysregulation can contribute to tumorigenesis through aberrant expression of genes controlling cell cycle and apoptosis. In cardiovascular disorders it impacts cardiac hypertrophy and heart failure due to its influence on heart-specific gene expression. HDAC7’s interaction with proteins like RUNX1 in T-cells can also contribute to the development of leukemia. These links highlight the importance of HDAC7 in both disease development and progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) (By similarity). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events (By similarity). Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Involved in muscle maturation by repressing transcription of myocyte enhancer factors such as MEF2A, MEF2B and MEF2C (By similarity). During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors (By similarity). May be involved in Epstein-Barr virus (EBV) latency, possibly by repressing the viral BZLF1 gene (PubMed : 12239305). Positively regulates the transcriptional repressor activity of FOXP3 (PubMed : 17360565). Serves as a corepressor of RARA, causing its deacetylation and inhibition of RARE DNA element binding (PubMed : 28167758). In association with RARA, plays a role in the repression of microRNA-10a and thereby in the inflammatory response (PubMed : 28167758). Also acetylates non-histone proteins, such as ALKBH5 (PubMed : 37369679).

See full target information HDAC7

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of neuroscience : the official journal 37:3848-3863 PubMed28283560

2017

HDAC7 Ubiquitination by the E3 Ligase CBX4 Is Involved in Contextual Fear Conditioning Memory Formation.

Applications

Unspecified application

Species

Unspecified reactive species

Xu Jing,Wen-Hai Sui,Shuai Wang,Xu-Feng Xu,Rong-Rong Yuan,Xiao-Rong Chen,Hui-Xian Ma,Ying-Xiao Zhu,Jin-Kai Sun,Fan Yi,Zhe-Yu Chen,Yue Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HDAC7 antibody - C-terminal

Immunocytochemistry/ Immunofluorescence - Anti-HDAC7 antibody - C-terminal (AB137366)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC7 antibody - C-terminal (AB137366)

Western blot - Anti-HDAC7 antibody - C-terminal (AB137366)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise