AB166911

Anti-HDAC7 antibody [EPR10922]

RabMAb
Recombinant
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(6 Publications)

Rabbit Recombinant Monoclonal HDAC7 antibody. Suitable for WB and reacts with Human samples. Cited in 6 publications.

View Alternative Names

HDAC7A, HDAC7, Histone deacetylase 7, HD7, Histone deacetylase 7A, Protein deacetylase HDAC7, HD7a

2 Images

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Western blot - Anti-HDAC7 antibody [EPR10922] (AB166911)

All lanes:

Western blot - Anti-HDAC7 antibody [EPR10922] (ab166911) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Human fetal brain tissue lysate at 10 µg

Lane 3:

A549 cell lysate at 10 µg

Lane 4:

K562 cell lysate at 10 µg

Predicted band size: 103 kDa

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CiteAb

Western blot - Anti-HDAC7 antibody [EPR10922] (AB166911)

HDAC7 western blot using anti-HDAC7 antibody [EPR10922] ab166911. Publication image and figure legend from Galán, M., Varona, S., et al., 2016, Dis Model Mech, PubMed 26989193.

ab166911 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab166911 please see the product overview.

Class I and II HDACs are upregulated in human abdominal aortic aneurysm (AAA). (A) Class I and IIa HDAC mRNA levels analysed by quantitative real-time PCR in abdominal aorta from AAA patients and donors (controls). Values shown are the mean±s.e.m. (AAA : n=22; donors : n=14). (B) Representative western blot analysis of HDAC1, 2, 4 and 7 (left panel) and their corresponding quantitative histogram (right panel; AAA : n=1; donors : n=8). (C) Representative immunostaining for HDAC1, 2, 4 and 7 in haematoxylin-counterstained aortic samples from AAA patients and controls. Scale bars : 50 μm. IC, inflammatory cells; VSMC, vascular smooth muscle cells. Arrows indicate positively stained cells. (D) Densitometric analysis of Ac-H3K18 protein levels analysed by western blot (shown in B) and immunohistochemistry in haematoxylin-counterstained aortic samples from AAA patients and controls. Results are expressed as mean±s.e.m. *P≤0.05 vs donors (t-test and one-way ANOVA).

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10922

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Histone deacetylase 7 (HDAC7) also known as HD7 or HD7A is a part of the class IIa histone deacetylases. It has a mass of approximately 103 kDa. HDAC7 plays a role in regulating gene expression by removing acetyl groups from lysine residues on histones leading to chromatin condensation and gene repression. This protein is expressed in various tissues including the heart skeletal muscle and T-cells where it influences diverse cellular processes.

Biological function summary

HDAC7 modulates transcription and affects cell differentiation and survival. It is a component of a larger protein complex working together with other proteins to influence transcription factor activity and chromatin architecture. It influences the expression of genes involved in immune responses as well as other critical cellular activities. By regulating these processes HDAC7 contributes to the balance between cell proliferation and apoptosis.

Pathways

HDAC7 plays key roles in the Wnt signaling and T-cell receptor (TCR) signaling pathways. It interacts with other proteins such as HDAC3 and the nuclear receptor co-repressor (N-CoR) affecting pathway components and influencing cellular outcomes. In the Wnt pathway HDAC7's activity modifies the transcription of target genes impacting cell fate decisions. During TCR signaling HDAC7 aids in the regulation of T-cell development and function working closely with transcriptional factors involved in these pathways.

HDAC7 has been associated with cancer and cardiovascular diseases. Its dysregulation can contribute to tumorigenesis through aberrant expression of genes controlling cell cycle and apoptosis. In cardiovascular disorders it impacts cardiac hypertrophy and heart failure due to its influence on heart-specific gene expression. HDAC7’s interaction with proteins like RUNX1 in T-cells can also contribute to the development of leukemia. These links highlight the importance of HDAC7 in both disease development and progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) (By similarity). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events (By similarity). Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Involved in muscle maturation by repressing transcription of myocyte enhancer factors such as MEF2A, MEF2B and MEF2C (By similarity). During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors (By similarity). May be involved in Epstein-Barr virus (EBV) latency, possibly by repressing the viral BZLF1 gene (PubMed : 12239305). Positively regulates the transcriptional repressor activity of FOXP3 (PubMed : 17360565). Serves as a corepressor of RARA, causing its deacetylation and inhibition of RARE DNA element binding (PubMed : 28167758). In association with RARA, plays a role in the repression of microRNA-10a and thereby in the inflammatory response (PubMed : 28167758). Also acetylates non-histone proteins, such as ALKBH5 (PubMed : 37369679).

See full target information HDAC7

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

CNS neuroscience & therapeutics 30:e14573 PubMed38421101

2024

HDAC9-mediated calmodulin deacetylation induces memory impairment in Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Hai-Long Zhang,Shufen Hu,Pin Yang,Han-Chun Long,Quan-Hong Ma,Dong-Min Yin,Guang-Yin Xu

Nature communications 14:3007 PubMed37230975

2023

HDAC9-mediated epithelial cell cycle arrest in G2/M contributes to kidney fibrosis in male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Zhang,Yujie Yang,Fan Yang,Xiaohan Liu,Ping Zhan,Jichao Wu,Xiaojie Wang,Ziying Wang,Wei Tang,Yu Sun,Yan Zhang,Qianqian Xu,Jin Shang,Junhui Zhen,Min Liu,Fan Yi

Investigative ophthalmology & visual science 61:17 PubMed32915982

2020

Histone Deacetylases Regulation by δ-Opioids in Human Optic Nerve Head Astrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Syed A H Zaidi,Nakul Thakore,Sudha Singh,Wendy Guzman,Shikhar Mehrotra,Vamsi Gangaraju,Shahid Husain

American journal of physiology. Renal physiology 316:F875-F888 PubMed30810062

2019

Dynamic changes in histone deacetylases following kidney ischemia-reperfusion injury are critical for promoting proximal tubule proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Kelly A Hyndman,Malgorzata Kasztan,Luciano D Mendoza,Sureena Monteiro-Pai

JCI insight 2:e90196 PubMed28352655

2017

Panobinostat acts synergistically with ibrutinib in diffuse large B cell lymphoma cells with MyD88 L265P mutations.

Applications

Species

Human

Patrizia Mondello,Elliott J Brea,Elisa De Stanchina,Eneda Toska,Aaron Y Chang,Myles Fennell,Venkatraman Seshan,Ralph Garippa,David A Scheinberg,José Baselga,Hans-Guido Wendel,Anas Younes

Disease models & mechanisms 9:541-52 PubMed26989193

2016

Induction of histone deacetylases (HDACs) in human abdominal aortic aneurysm: therapeutic potential of HDAC inhibitors.

Applications

Species

Unspecified reactive species

María Galán,Saray Varona,Mar Orriols,José Antonio Rodríguez,Silvia Aguiló,Jaume Dilmé,Mercedes Camacho,José Martínez-González,Cristina Rodriguez

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HDAC7 antibody [EPR10922]

Western blot - Anti-HDAC7 antibody [EPR10922] (AB166911)

Western blot - Anti-HDAC7 antibody [EPR10922] (AB166911)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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