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AB249392

Anti-HDAC7 antibody [EPR10922] - BSA and Azide free

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Rabbit Recombinant Monoclonal HDAC7 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

HDAC7A, HDAC7, Histone deacetylase 7, HD7, Histone deacetylase 7A, Protein deacetylase HDAC7, HD7a

1 Images
Western blot - Anti-HDAC7 antibody [EPR10922] - BSA and Azide free (AB249392)
  • WB

Unknown

Western blot - Anti-HDAC7 antibody [EPR10922] - BSA and Azide free (AB249392)

This data was developed using ab166911, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-HDAC7 antibody [EPR10922] (<a href='/en-us/products/primary-antibodies/hdac7-antibody-epr10922-ab166911'>ab166911</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Human fetal brain tissue lysate at 10 µg

Lane 3:

A549 cell lysate at 10 µg

Lane 4:

K562 cell lysate at 10 µg

Predicted band size: 103 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10922

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab249392 is the carrier-free version of ab166911.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Histone deacetylase 7 (HDAC7) also known as HD7 or HD7A is a part of the class IIa histone deacetylases. It has a mass of approximately 103 kDa. HDAC7 plays a role in regulating gene expression by removing acetyl groups from lysine residues on histones leading to chromatin condensation and gene repression. This protein is expressed in various tissues including the heart skeletal muscle and T-cells where it influences diverse cellular processes.
Biological function summary

HDAC7 modulates transcription and affects cell differentiation and survival. It is a component of a larger protein complex working together with other proteins to influence transcription factor activity and chromatin architecture. It influences the expression of genes involved in immune responses as well as other critical cellular activities. By regulating these processes HDAC7 contributes to the balance between cell proliferation and apoptosis.

Pathways

HDAC7 plays key roles in the Wnt signaling and T-cell receptor (TCR) signaling pathways. It interacts with other proteins such as HDAC3 and the nuclear receptor co-repressor (N-CoR) affecting pathway components and influencing cellular outcomes. In the Wnt pathway HDAC7's activity modifies the transcription of target genes impacting cell fate decisions. During TCR signaling HDAC7 aids in the regulation of T-cell development and function working closely with transcriptional factors involved in these pathways.

HDAC7 has been associated with cancer and cardiovascular diseases. Its dysregulation can contribute to tumorigenesis through aberrant expression of genes controlling cell cycle and apoptosis. In cardiovascular disorders it impacts cardiac hypertrophy and heart failure due to its influence on heart-specific gene expression. HDAC7’s interaction with proteins like RUNX1 in T-cells can also contribute to the development of leukemia. These links highlight the importance of HDAC7 in both disease development and progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) (By similarity). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events (By similarity). Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Involved in muscle maturation by repressing transcription of myocyte enhancer factors such as MEF2A, MEF2B and MEF2C (By similarity). During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors (By similarity). May be involved in Epstein-Barr virus (EBV) latency, possibly by repressing the viral BZLF1 gene (PubMed : 12239305). Positively regulates the transcriptional repressor activity of FOXP3 (PubMed : 17360565). Serves as a corepressor of RARA, causing its deacetylation and inhibition of RARE DNA element binding (PubMed : 28167758). In association with RARA, plays a role in the repression of microRNA-10a and thereby in the inflammatory response (PubMed : 28167758). Also acetylates non-histone proteins, such as ALKBH5 (PubMed : 37369679).
See full target information HDAC7
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Product promise

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