AB217702

Anti-HDAC8 antibody

Name: Anti-HDAC8 antibody (ab217702) | Abcam
Brand: Abcam Limited
SKU: AB217702
Price: 525 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal HDAC8 antibody. Suitable for IHC-P and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human HDAC8 aa 150-250 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

HDACL1, CDA07, HDAC8, Histone deacetylase 8, HD8, Protein deacetylase HDAC8, Protein decrotonylase HDAC8

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC8 antibody (AB217702)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC8 antibody (AB217702)

Immunohistochemical analysis of formalin-fixed paraffin-embedded human brain tissue labeling HDAC8 with ab217702 at a 1/200 dilution, followed by a secondary antibody and DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC8 antibody (AB217702)

Immunohistochemical analysis of formalin-fixed paraffin-embedded mouse pancreas tissue labeling HDAC8 with ab217702 at 1/200 dilution, followed by a secondary antibody and DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human HDAC8 aa 150-250 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q9BY41

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein HDAC8 also known as Histone Deacetylase 8 functions mechanically by removing acetyl groups from histone tails influencing chromatin structure and gene expression. HDAC8 has a molecular mass of roughly 41 kDa. It expresses in various tissues but shows higher expression in smooth muscle cells and certain cancer cell lines. Researchers also note its expression in the brain and liver corresponding to sections that require its deacetylase activity for various regulatory functions.

Biological function summary

HDAC8 plays a role in regulating transcription by being involved in altering chromatin structure. It does not form part of a large multi-protein complex unlike some other histone deacetylases. Its activity affects cell cycle progression and differentiation which is central to cellular physiology. HDAC8 also participates in non-histone protein deacetylation impacting various substrates allowing it to govern diverse cellular processes.

Pathways

Scientists associate HDAC8 with cell cycle regulation and muscle contraction. HDAC8 plays a role in the molecular pathways that control how myosin light chains undergo deacetylation interacting with proteins such as smooth muscle actin. During cell cycle regulation HDAC8 affects p53 a protein critical in controlling cell cycle progression and apoptosis integrating into pathways that decide cellular outcomes during division and response to stress.

Researchers identify HDAC8 as having connections to certain cancers and Cornelia de Lange Syndrome (CdLS). Its deregulation links to tumorigenesis particularly in colorectal and neuroblastoma cancers where it may interact with oncogenes and tumor suppressors. In CdLS mutations that affect HDAC8 lead to developmental issues with the disorder being connected to the cohesin complex proteins which highlight HDAC8's importance in chromosomal cohesion and gene expression regulation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Histone deacetylase that catalyzes the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) (PubMed : 10748112, PubMed : 10922473, PubMed : 10926844, PubMed : 14701748, PubMed : 28497810). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events (PubMed : 10748112, PubMed : 10922473, PubMed : 10926844, PubMed : 14701748). Histone deacetylases act via the formation of large multiprotein complexes (PubMed : 10748112, PubMed : 10922473, PubMed : 10926844, PubMed : 14701748). Also involved in the deacetylation of cohesin complex protein SMC3 regulating release of cohesin complexes from chromatin (PubMed : 22885700). May play a role in smooth muscle cell contractility (PubMed : 15772115). In addition to protein deacetylase activity, also has protein-lysine deacylase activity : acts as a protein decrotonylase by mediating decrotonylation ((2E)-butenoyl) of histones (PubMed : 28497810).

See full target information HDAC8

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cells 12: PubMed37887281

2023

Enhanced Transcriptional Signature and Expression of Histone-Modifying Enzymes in Salivary Gland Tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Manou,Theodoros Loupis,Dimitrios M Vrachnos,Nikolaos Katsoulas,Stamatios Theocharis,Dimitrios S Kanakoglou,Efthimia K Basdra,Christina Piperi,Athanasios G Papavassiliou

Translational cancer research 9:1406-1417 PubMed35117488

2020

Over-expression of HDAC8 down-regulate CDKN2A is associated with worse prognosis of esophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Effat Un Nesa,Xuan Chen,Cong Wang,Xue Chen,Yuan Wang,Yan Qu,Si Mi,Shanghai Guan,Fengxia Xiao,Yufeng Cheng

Oncology letters 16:6552-6560 PubMed30405794

2018

HDAC inhibitor apicidin suppresses murine oral squamous cell carcinoma cell growth and via inhibiting HDAC8 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Mee-Young Ahn

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HDAC8 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC8 antibody (AB217702)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC8 antibody (AB217702)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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