Rabbit Recombinant Monoclonal HDAC8 antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 33 publications.
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
IP | WB | Flow Cyt (Intra) | |
---|---|---|---|
Human | Tested | Tested | Tested |
Mouse | Expected | Expected | Expected |
Rat | Predicted | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/40 - 1/60 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/40 - 1/60 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/10000 - 1/50000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/10000 - 1/50000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/150 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/150 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info - | Notes - |
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Histone deacetylase that catalyzes the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) (PubMed:10748112, PubMed:10922473, PubMed:10926844, PubMed:14701748, PubMed:28497810). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events (PubMed:10748112, PubMed:10922473, PubMed:10926844, PubMed:14701748). Histone deacetylases act via the formation of large multiprotein complexes (PubMed:10748112, PubMed:10922473, PubMed:10926844, PubMed:14701748). Also involved in the deacetylation of cohesin complex protein SMC3 regulating release of cohesin complexes from chromatin (PubMed:22885700). May play a role in smooth muscle cell contractility (PubMed:15772115). In addition to protein deacetylase activity, also has protein-lysine deacylase activity: acts as a protein decrotonylase by mediating decrotonylation ((2E)-butenoyl) of histones (PubMed:28497810).
HDACL1, CDA07, HDAC8, Histone deacetylase 8, HD8, Protein deacetylase HDAC8, Protein decrotonylase HDAC8
Rabbit Recombinant Monoclonal HDAC8 antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 33 publications.
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
The protein HDAC8 also known as Histone Deacetylase 8 functions mechanically by removing acetyl groups from histone tails influencing chromatin structure and gene expression. HDAC8 has a molecular mass of roughly 41 kDa. It expresses in various tissues but shows higher expression in smooth muscle cells and certain cancer cell lines. Researchers also note its expression in the brain and liver corresponding to sections that require its deacetylase activity for various regulatory functions.
HDAC8 plays a role in regulating transcription by being involved in altering chromatin structure. It does not form part of a large multi-protein complex unlike some other histone deacetylases. Its activity affects cell cycle progression and differentiation which is central to cellular physiology. HDAC8 also participates in non-histone protein deacetylation impacting various substrates allowing it to govern diverse cellular processes.
Scientists associate HDAC8 with cell cycle regulation and muscle contraction. HDAC8 plays a role in the molecular pathways that control how myosin light chains undergo deacetylation interacting with proteins such as smooth muscle actin. During cell cycle regulation HDAC8 affects p53 a protein critical in controlling cell cycle progression and apoptosis integrating into pathways that decide cellular outcomes during division and response to stress.
Researchers identify HDAC8 as having connections to certain cancers and Cornelia de Lange Syndrome (CdLS). Its deregulation links to tumorigenesis particularly in colorectal and neuroblastoma cancers where it may interact with oncogenes and tumor suppressors. In CdLS mutations that affect HDAC8 lead to developmental issues with the disorder being connected to the cohesin complex proteins which highlight HDAC8's importance in chromosomal cohesion and gene expression regulation.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Immunoprecipitation analysis of human fetal kidney tissue lysate labeling HDAC8 using ab187139 at 1/50 dilution (Lane 1). Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody. Lane 2: PBS instead of human fetal kidney tissue lysate.
All lanes: Immunoprecipitation - Anti-HDAC8 antibody [EPR10338(2)] (ab187139)
Predicted band size: 42 kDa
All lanes: Western blot - Anti-HDAC8 antibody [EPR10338(2)] (ab187139) at 1/50000 dilution
Lane 1: K562 cell lysate at 20 µg
Lane 2: Molt4 cell lysate at 20 µg
Lane 3: HeLa cell lysate at 20 µg
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 42 kDa
Intracellular Flow Cytometry analysis of K562 cells labeling HDAC8 using ab187139 at 1/150 dilution. A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Isotype control: Rabbit monoclonal IgG.
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