Anti-HDAC8 antibody [EPR10338(2)]

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-HDAC8 antibody [EPR10338(2)] (AB187139)

Intracellular Flow Cytometry analysis of K562 cells labeling HDAC8 using ab187139 at 1/150 dilution. A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Isotype control : Rabbit monoclonal IgG.

• IP

Supplier Data

Immunoprecipitation - Anti-HDAC8 antibody [EPR10338(2)] (AB187139)

Immunoprecipitation analysis of human fetal kidney tissue lysate labeling HDAC8 using ab187139 at 1/50 dilution (Lane 1). Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody. Lane 2 : PBS instead of human fetal kidney tissue lysate.

All lanes:

Immunoprecipitation - Anti-HDAC8 antibody [EPR10338(2)] (ab187139)

Predicted band size: 42 kDa

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• WB

Supplier Data

Western blot - Anti-HDAC8 antibody [EPR10338(2)] (AB187139)

All lanes:

Western blot - Anti-HDAC8 antibody [EPR10338(2)] (ab187139) at 1/50000 dilution

Lane 1:

K562 cell lysate at 20 µg

Lane 2:

Molt4 cell lysate at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 42 kDa

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• WB

Lab

Western blot - Anti-HDAC8 antibody [EPR10338(2)] (AB187139)

Western blot : Rabbit Monoclonal [EPR10338(2)] to HDAC8 ab187139 staining at 1/10000 dilution, shown in green; Mouse anti-CANX (ab238078) loading control staining at 1/20,000 dilution, shown in magenta.

A band was observed at 42 kDa in Wild-type U-87 MG cell lysates with no signal observed at this size in HDAC8 knockout U-87 MG cell line.

To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.

Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-HDAC8 antibody [EPR10338(2)] (ab187139) at 1/10000 dilution

Lane 1:

Wild-type U-87 MG at 20 µg

Lane 2:

HDAC8 knockout U-87 MG at 20 µg

Lane 3:

HeLa at 20 µg

Lane 4:

HCT 116 at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 42 kDa

Observed band size: 42 kDa

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