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Rabbit Recombinant Monoclonal HDAC8 antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 33 publications.


Images

Immunoprecipitation - Anti-HDAC8 antibody [EPR10338(2)] (AB187139), expandable thumbnail
  • Western blot - Anti-HDAC8 antibody [EPR10338(2)] (AB187139), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-HDAC8 antibody [EPR10338(2)] (AB187139), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Abcam Recommends

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBFlow Cyt (Intra)
Human
Tested
Tested
Tested
Mouse
Expected
Expected
Expected
Rat
Predicted
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1/40 - 1/60
Notes

-

Expected
Expected

Species
Mouse
Dilution info
1/40 - 1/60
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10000 - 1/50000
Notes

-

Expected
Expected

Species
Mouse
Dilution info
1/10000 - 1/50000
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/150
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Mouse
Dilution info
1/150
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Histone deacetylase that catalyzes the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) (PubMed:10748112, PubMed:10922473, PubMed:10926844, PubMed:14701748, PubMed:28497810). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events (PubMed:10748112, PubMed:10922473, PubMed:10926844, PubMed:14701748). Histone deacetylases act via the formation of large multiprotein complexes (PubMed:10748112, PubMed:10922473, PubMed:10926844, PubMed:14701748). Also involved in the deacetylation of cohesin complex protein SMC3 regulating release of cohesin complexes from chromatin (PubMed:22885700). May play a role in smooth muscle cell contractility (PubMed:15772115). In addition to protein deacetylase activity, also has protein-lysine deacylase activity: acts as a protein decrotonylase by mediating decrotonylation ((2E)-butenoyl) of histones (PubMed:28497810).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal HDAC8 antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 33 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR10338(2)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The protein HDAC8 also known as Histone Deacetylase 8 functions mechanically by removing acetyl groups from histone tails influencing chromatin structure and gene expression. HDAC8 has a molecular mass of roughly 41 kDa. It expresses in various tissues but shows higher expression in smooth muscle cells and certain cancer cell lines. Researchers also note its expression in the brain and liver corresponding to sections that require its deacetylase activity for various regulatory functions.

Biological function summary

HDAC8 plays a role in regulating transcription by being involved in altering chromatin structure. It does not form part of a large multi-protein complex unlike some other histone deacetylases. Its activity affects cell cycle progression and differentiation which is central to cellular physiology. HDAC8 also participates in non-histone protein deacetylation impacting various substrates allowing it to govern diverse cellular processes.

Pathways

Scientists associate HDAC8 with cell cycle regulation and muscle contraction. HDAC8 plays a role in the molecular pathways that control how myosin light chains undergo deacetylation interacting with proteins such as smooth muscle actin. During cell cycle regulation HDAC8 affects p53 a protein critical in controlling cell cycle progression and apoptosis integrating into pathways that decide cellular outcomes during division and response to stress.

Associated diseases and disorders

Researchers identify HDAC8 as having connections to certain cancers and Cornelia de Lange Syndrome (CdLS). Its deregulation links to tumorigenesis particularly in colorectal and neuroblastoma cancers where it may interact with oncogenes and tumor suppressors. In CdLS mutations that affect HDAC8 lead to developmental issues with the disorder being connected to the cohesin complex proteins which highlight HDAC8's importance in chromosomal cohesion and gene expression regulation.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunoprecipitation - Anti-HDAC8 antibody [EPR10338(2)] (ab187139), expandable thumbnail

    Immunoprecipitation - Anti-HDAC8 antibody [EPR10338(2)] (ab187139)

    Immunoprecipitation analysis of human fetal kidney tissue lysate labeling HDAC8 using ab187139 at 1/50 dilution (Lane 1). Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody. Lane 2: PBS instead of human fetal kidney tissue lysate.

    All lanes: Immunoprecipitation - Anti-HDAC8 antibody [EPR10338(2)] (ab187139)

    Predicted band size: 42 kDa

  • Western blot - Anti-HDAC8 antibody [EPR10338(2)] (ab187139), expandable thumbnail

    Western blot - Anti-HDAC8 antibody [EPR10338(2)] (ab187139)

    All lanes: Western blot - Anti-HDAC8 antibody [EPR10338(2)] (ab187139) at 1/50000 dilution

    Lane 1: K562 cell lysate at 20 µg

    Lane 2: Molt4 cell lysate at 20 µg

    Lane 3: HeLa cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 42 kDa

  • Flow Cytometry (Intracellular) - Anti-HDAC8 antibody [EPR10338(2)] (ab187139), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-HDAC8 antibody [EPR10338(2)] (ab187139)

    Intracellular Flow Cytometry analysis of K562 cells labeling HDAC8 using ab187139 at 1/150 dilution. A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Isotype control: Rabbit monoclonal IgG.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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