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Rabbit Recombinant Monoclonal HDAC9 antibody. Carrier free. Suitable for IHC-P, ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples.

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Images

Western blot - Anti-HDAC9 antibody [EPR5223] - BSA and Azide free (AB239979), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-HDAC9 antibody [EPR5223] - BSA and Azide free (AB239979), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-HDAC9 antibody [EPR5223] - BSA and Azide free (AB239979), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC9 antibody [EPR5223] - BSA and Azide free (AB239979), expandable thumbnail
  • Western blot - Anti-HDAC9 antibody [EPR5223] - BSA and Azide free (AB239979), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFIPWBFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

8 products for Alternative Version

Target data

Function

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Represses MEF2-dependent transcription. Isoform 3 lacks active site residues and therefore is catalytically inactive. Represses MEF2-dependent transcription by recruiting HDAC1 and/or HDAC3. Seems to inhibit skeletal myogenesis and to be involved in heart development. Protects neurons from apoptosis, both by inhibiting JUN phosphorylation by MAPK10 and by repressing JUN transcription via HDAC1 recruitment to JUN promoter.

Alternative names

HDAC7, HDAC7B, HDRP, KIAA0744, MITR, HDAC9, Histone deacetylase 9, HD9, Histone deacetylase 7B, Histone deacetylase-related protein, MEF2-interacting transcription repressor MITR, HD7, HD7b

Recommended products

Rabbit Recombinant Monoclonal HDAC9 antibody. Carrier free. Suitable for IHC-P, ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR5223
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab239979 is the carrier-free version of Anti-HDAC9 antibody [EPR5223] ab109446.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

HDAC9 also known as Histone Deacetylase 9 is a protein involved in removing acetyl groups from histone proteins leading to chromatin condensation and transcriptional repression. HDAC9 has a molecular weight of about 127 kDa. It expresses predominantly in the brain muscle tissue and immune cells. This protein belongs to the class IIa family of histone deacetylases and has close structural similarities to other class IIa HDACs like HDAC4 HDAC5 and HDAC7.

Biological function summary

HDAC9 plays a significant role in regulating gene expression by altering the acetylation status of histones. It participates in the formation of large multiprotein complexes that target specific gene promoters. HDAC9's activity impacts various cellular processes including cell cycle progression and differentiation. It is a part of the CoREST complex which interacts with REST corepressor 1 to modulate neuronal gene expression and synaptic plasticity.

Pathways

HDAC9 is actively involved in key cellular signaling cascades. It contributes to the Notch signaling pathway which has a profound impact on cell differentiation and fate decisions. It also plays a role in the NF-kB pathway which is critical for immune response and inflammation. Through these pathways HDAC9 interacts with proteins such as RBP-Jk in the Notch pathway and IKK complex members in the NF-kB pathway indicating its broad influence on cellular mechanisms.

Associated diseases and disorders

HDAC9 associates with several pathological conditions. It has been linked to cardiovascular diseases such as cardiac hypertrophy and heart failure due to its influence on cardiac muscle gene expression. HDAC9 also relates to various cancers including colon cancer where its dysregulation affects tumorigenesis and cell proliferation. In these contexts HDAC9 interacts with proteins like MEF2 and p300 which are pivotal in cardiac growth and cancer progression respectively.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-HDAC9 antibody [EPR5223] - BSA and Azide free (ab239979), expandable thumbnail

    Western blot - Anti-HDAC9 antibody [EPR5223] - BSA and Azide free (ab239979)

    All lanes: Western blot - Anti-HDAC9 antibody [EPR5223] (Anti-HDAC9 antibody [EPR5223] ab109446) at 1/1000 dilution

    Lane 1: Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg

    Lane 2: K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 111 kDa

  • Flow Cytometry (Intracellular) - Anti-HDAC9 antibody [EPR5223] - BSA and Azide free (ab239979), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-HDAC9 antibody [EPR5223] - BSA and Azide free (ab239979)

    This data was developed using ab239979, the same antibody clone in a different buffer formulation.
    Flow Cytometry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labelling HDAC9 with Purified ab239979 at 1:100 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-HDAC9 antibody [EPR5223] - BSA and Azide free (ab239979), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-HDAC9 antibody [EPR5223] - BSA and Azide free (ab239979)

    This data was developed using ab239979, the same antibody clone in a different buffer formulation.
    Immunocytochemistry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labeling HDAC9 with Purified ab239979 at 1:100 dilution (10 μg/ml). Cells were fixed in 100% Methanol and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC9 antibody [EPR5223] - BSA and Azide free (ab239979), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC9 antibody [EPR5223] - BSA and Azide free (ab239979)

    This data was developed using Anti-HDAC9 antibody [EPR5223] ab109446, the same antibody clone in a different buffer formulation.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling HDAC9 with Purified Anti-HDAC9 antibody [EPR5223] ab109446 at 1:1000 dilution (1.10 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Western blot - Anti-HDAC9 antibody [EPR5223] - BSA and Azide free (ab239979), expandable thumbnail

    Western blot - Anti-HDAC9 antibody [EPR5223] - BSA and Azide free (ab239979)

    This data was developed using Anti-HDAC9 antibody [EPR5223] ab109446, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 whole cell lysate (20 μg)

    Lane 2: HDAC9 (KO) knockout HAP1 whole cell lysate (20 μg)

    Lane 3: HepG2 whole cell lysate (20 μg)

    Lane 4: Raji whole cell lysate (20 μg)

    Lanes 1 - 4: Merged signal (red and green). Green - Anti-HDAC9 antibody [EPR5223] ab109446 observed at 140 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.

    Anti-HDAC9 antibody [EPR5223] ab109446 was shown to specifically recognize HDAC9 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when HDAC9 knockout samples were usedexamined. Wild-type and HDAC9 knockout samples were subjected to SDS-PAGE. Anti-HDAC9 antibody [EPR5223] ab109446 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/10,000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-HDAC9 antibody [EPR5223] (Anti-HDAC9 antibody [EPR5223] ab109446)

    Predicted band size: 111 kDa

    Observed band size: 140 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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